DNA methylation is involved in the aberrant expression of miR-133b in colorectal cancer cells

  • Authors:
    • Lv Lv
    • Jianyu Zhou
    • Changwei Lin
    • Gui Hu
    • Lu Yi
    • Juan Du
    • Kai Gao
    • Xiaorong Li
  • View Affiliations

  • Published online on: June 8, 2015     https://doi.org/10.3892/ol.2015.3336
  • Pages: 907-912
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Abstract

The dysregulation of microRNA (miRNA) expression is highly involved in cancer. Recently, a number of studies have demonstrated that the silencing of specific miRNAs is associated with DNA methylation. The muscle‑specific miRNA‑113b (miR‑133b) is markedly downregulated in human colorectal cancer (CRC) compared with healthy colon cells, and is critical in the regulation of CRC cell proliferation and apoptosis. However, the mechanism of miR‑133b downregulation in CRC has yet to be elucidated. Therefore, the aim of the present study was to determine the existence of an association between DNA methylation and miR‑133b expression in CRC cells. It was identified that miR‑133b promoter hypermethylation is upregulated in CRC tissues. To investigate the role of miR‑133b methylation in CRC cells, the survival, cell cycle and invasion were analyzed in HT‑29 and SW620 CRC cells treated with 5‑aza‑2'‑deoxycytidine (5‑Aza‑CdR), 4‑phenylbutyric acid (PBA) and 5‑Aza‑CdR/PBA. Functional analysis demonstrated that demethylation increased the expression of miR‑133b, which restored migration and apoptosis in CRC cells. Thus, these results indicate that the regulation of miR‑133b methylation may provide a novel therapeutic strategy for CRC treatment.
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August-2015
Volume 10 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lv L, Zhou J, Lin C, Hu G, Yi L, Du J, Gao K and Li X: DNA methylation is involved in the aberrant expression of miR-133b in colorectal cancer cells. Oncol Lett 10: 907-912, 2015
APA
Lv, L., Zhou, J., Lin, C., Hu, G., Yi, L., Du, J. ... Li, X. (2015). DNA methylation is involved in the aberrant expression of miR-133b in colorectal cancer cells. Oncology Letters, 10, 907-912. https://doi.org/10.3892/ol.2015.3336
MLA
Lv, L., Zhou, J., Lin, C., Hu, G., Yi, L., Du, J., Gao, K., Li, X."DNA methylation is involved in the aberrant expression of miR-133b in colorectal cancer cells". Oncology Letters 10.2 (2015): 907-912.
Chicago
Lv, L., Zhou, J., Lin, C., Hu, G., Yi, L., Du, J., Gao, K., Li, X."DNA methylation is involved in the aberrant expression of miR-133b in colorectal cancer cells". Oncology Letters 10, no. 2 (2015): 907-912. https://doi.org/10.3892/ol.2015.3336