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Zinc finger protein X-linked is overexpressed in colorectal cancer and is associated with poor prognosis

  • Authors:
    • Jin Jiang
    • Lu‑Ying Liu
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, The First Hospital of Jiaxing, Jiaxing, Zhejiang 310012, P.R. China, Department of Radiation Oncology, Zhejiang Key Laboratory of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 810-814
    |
    Published online on: June 10, 2015
       https://doi.org/10.3892/ol.2015.3353
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Abstract

Zinc finger protein X-linked (ZFX) is a zinc finger transcription factor and plays a significant role in the self‑renewal ability of embryonic stem cells and various cancers. However, its expression and function in colorectal cancer (CRC) remain unclear. In the present study, we evaluated the expression of ZFX in CRC using quantitative polymerase chain reaction (qPCR), western blot analysis and immunohistochemistry (IHC), and further explored its potential functions in CRC cell lines using cell counting kit‑8 and Transwell invasion assays. qPCR and western blot analysis revealed that ZFX was significantly upregulated in CRC tissues; IHC further confirmed this finding, revealing that higher expression of ZFX was significantly associated with larger tumor size (P=0.01), higher pathological stage (P=0.02), depth of invasion (P=0.047), lymph node invasion (P=0.02) and higher American Joint Committee on Cancer (AJCC) stage (P=0.04). CRC patients with higher ZFX expression also exhibited significantly shorter survival times (P=0.019). Moreover, knockdown of ZFX significantly suppressed proliferation and invasion in CRC cell lines HCT116 and LoVo. These results suggest that ZFX plays a notable role in CRC tumorigenicity and may serve as a novel marker and therapeutic target for CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang J and Liu LY: Zinc finger protein X-linked is overexpressed in colorectal cancer and is associated with poor prognosis. Oncol Lett 10: 810-814, 2015.
APA
Jiang, J., & Liu, L. (2015). Zinc finger protein X-linked is overexpressed in colorectal cancer and is associated with poor prognosis. Oncology Letters, 10, 810-814. https://doi.org/10.3892/ol.2015.3353
MLA
Jiang, J., Liu, L."Zinc finger protein X-linked is overexpressed in colorectal cancer and is associated with poor prognosis". Oncology Letters 10.2 (2015): 810-814.
Chicago
Jiang, J., Liu, L."Zinc finger protein X-linked is overexpressed in colorectal cancer and is associated with poor prognosis". Oncology Letters 10, no. 2 (2015): 810-814. https://doi.org/10.3892/ol.2015.3353
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang J and Liu LY: Zinc finger protein X-linked is overexpressed in colorectal cancer and is associated with poor prognosis. Oncol Lett 10: 810-814, 2015.
APA
Jiang, J., & Liu, L. (2015). Zinc finger protein X-linked is overexpressed in colorectal cancer and is associated with poor prognosis. Oncology Letters, 10, 810-814. https://doi.org/10.3892/ol.2015.3353
MLA
Jiang, J., Liu, L."Zinc finger protein X-linked is overexpressed in colorectal cancer and is associated with poor prognosis". Oncology Letters 10.2 (2015): 810-814.
Chicago
Jiang, J., Liu, L."Zinc finger protein X-linked is overexpressed in colorectal cancer and is associated with poor prognosis". Oncology Letters 10, no. 2 (2015): 810-814. https://doi.org/10.3892/ol.2015.3353
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