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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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September-2015 Volume 10 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

Piwil2 modulates the invasion and metastasis of prostate cancer by regulating the expression of matrix metalloproteinase-9 and epithelial-mesenchymal transitions

  • Authors:
    • Yanfeng Yang
    • Xuepei Zhang
    • Dongkui Song
    • Jinxing Wei
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China
  • Pages: 1735-1740
    |
    Published online on: June 19, 2015
       https://doi.org/10.3892/ol.2015.3392
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Abstract

Piwi-like RNA-mediated gene silencing 2 (Piwil2) is an oncogene that is highly expressed in breast, gastric, colorectal and papillary thyroid cancer. As a candidate oncogene, its role in prostrate cancer has yet to be elucidated. In the present study, 30 tumor specimens and four prostate cancer cell lines were analyzed. The total RNA and protein from the specimens and the cells were analyzed using quantitative polymerase chain reaction and western blotting, respectively. The expression of Piwil2 in PC‑3 cells was knocked down using specific small hairpin RNA. Transwell assays and wound‑healing models were used to assess cell invasion and migration. In addition, the expression of several factors associated with epithelial‑mesenchymal transitions (EMT) were evaluated by western blotting. The results revealed that the Piwil2 gene was associated with the Gleason score and the tumor‑node‑metastasis stage of the tumor tissues. Cell invasion and migration decreased significantly in PC‑3 cells with knocked-down Piwil2. In addition, silencing Piwil2 downregulated the expression of N‑cadherin, Twist and vimentin and upregulated the expression of E‑cadherin, factors associated with EMT, and also reduced the expression of matrix metalloproteinase‑9. Piwil2 was demonstrated to possess an important role in the invasive ability of prostate cancer, and therefore, may be a potential therapeutic target for the treatment of this cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Zhang X, Song D and Wei J: Piwil2 modulates the invasion and metastasis of prostate cancer by regulating the expression of matrix metalloproteinase-9 and epithelial-mesenchymal transitions. Oncol Lett 10: 1735-1740, 2015.
APA
Yang, Y., Zhang, X., Song, D., & Wei, J. (2015). Piwil2 modulates the invasion and metastasis of prostate cancer by regulating the expression of matrix metalloproteinase-9 and epithelial-mesenchymal transitions. Oncology Letters, 10, 1735-1740. https://doi.org/10.3892/ol.2015.3392
MLA
Yang, Y., Zhang, X., Song, D., Wei, J."Piwil2 modulates the invasion and metastasis of prostate cancer by regulating the expression of matrix metalloproteinase-9 and epithelial-mesenchymal transitions". Oncology Letters 10.3 (2015): 1735-1740.
Chicago
Yang, Y., Zhang, X., Song, D., Wei, J."Piwil2 modulates the invasion and metastasis of prostate cancer by regulating the expression of matrix metalloproteinase-9 and epithelial-mesenchymal transitions". Oncology Letters 10, no. 3 (2015): 1735-1740. https://doi.org/10.3892/ol.2015.3392
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Zhang X, Song D and Wei J: Piwil2 modulates the invasion and metastasis of prostate cancer by regulating the expression of matrix metalloproteinase-9 and epithelial-mesenchymal transitions. Oncol Lett 10: 1735-1740, 2015.
APA
Yang, Y., Zhang, X., Song, D., & Wei, J. (2015). Piwil2 modulates the invasion and metastasis of prostate cancer by regulating the expression of matrix metalloproteinase-9 and epithelial-mesenchymal transitions. Oncology Letters, 10, 1735-1740. https://doi.org/10.3892/ol.2015.3392
MLA
Yang, Y., Zhang, X., Song, D., Wei, J."Piwil2 modulates the invasion and metastasis of prostate cancer by regulating the expression of matrix metalloproteinase-9 and epithelial-mesenchymal transitions". Oncology Letters 10.3 (2015): 1735-1740.
Chicago
Yang, Y., Zhang, X., Song, D., Wei, J."Piwil2 modulates the invasion and metastasis of prostate cancer by regulating the expression of matrix metalloproteinase-9 and epithelial-mesenchymal transitions". Oncology Letters 10, no. 3 (2015): 1735-1740. https://doi.org/10.3892/ol.2015.3392
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