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Article Open Access

Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues

  • Authors:
    • Zhenxin Wang
    • Jianfeng Yang
    • Yanbo Zhu
    • Yun Zhu
    • Bin Zhang
    • Yinghui Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Cyrus Tang Hematology Center, Soochow University, Suzhou, Jiangsu 215123, P.R. China, Department of Cerebral Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Department of Biochemistry and Molecular Biochemistry, School of Medicine, Soochow University, Suzhou, Jiangsu 215123, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2204-2208
    |
    Published online on: August 14, 2015
       https://doi.org/10.3892/ol.2015.3611
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Abstract

B7‑H3 protein is an important tumor antigen, but the expression of its isoforms, 4IgB7‑H3 and 2IgB7‑H3, in tumor tissues remains unknown due to the lack of specific monoclonal antibodies (mAbs). In the present study, a mAb (9C3) specifically recognizing 2IgB7‑H3, but not 4IgB7‑H3, was prepared. Using 9C3 and a previously prepared mAb (4H7) that recognizes 4IgB7‑H3 and 2IgB7‑H3, the differential expression of 2IgB7‑H3 and 4IgB7‑H3 was analyzed in a variety of tumor cell lines by flow cytometry. It was found that 4IgB7‑H3 had a more broad spectrum of expression among the cell lines compared with 2IgB7‑H3. The expression of the two isoforms was further examined in glioma tissues using reverse transcription‑polymerase chain reaction and immunohistochemistry techniques. The data revealed that 2IgB7‑H3, but not 4IgB7‑H3, was specifically expressed in glioma. Taken together, these results demonstrated for the first time that 2IgB7‑H3 is a valuable biomarker for the diagnosis of glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Yang J, Zhu Y, Zhu Y, Zhang B and Zhou Y: Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues. Oncol Lett 10: 2204-2208, 2015.
APA
Wang, Z., Yang, J., Zhu, Y., Zhu, Y., Zhang, B., & Zhou, Y. (2015). Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues. Oncology Letters, 10, 2204-2208. https://doi.org/10.3892/ol.2015.3611
MLA
Wang, Z., Yang, J., Zhu, Y., Zhu, Y., Zhang, B., Zhou, Y."Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues". Oncology Letters 10.4 (2015): 2204-2208.
Chicago
Wang, Z., Yang, J., Zhu, Y., Zhu, Y., Zhang, B., Zhou, Y."Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues". Oncology Letters 10, no. 4 (2015): 2204-2208. https://doi.org/10.3892/ol.2015.3611
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Yang J, Zhu Y, Zhu Y, Zhang B and Zhou Y: Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues. Oncol Lett 10: 2204-2208, 2015.
APA
Wang, Z., Yang, J., Zhu, Y., Zhu, Y., Zhang, B., & Zhou, Y. (2015). Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues. Oncology Letters, 10, 2204-2208. https://doi.org/10.3892/ol.2015.3611
MLA
Wang, Z., Yang, J., Zhu, Y., Zhu, Y., Zhang, B., Zhou, Y."Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues". Oncology Letters 10.4 (2015): 2204-2208.
Chicago
Wang, Z., Yang, J., Zhu, Y., Zhu, Y., Zhang, B., Zhou, Y."Differential expression of 2IgB7‑H3 and 4IgB7‑H3 in cancer cell lines and glioma tissues". Oncology Letters 10, no. 4 (2015): 2204-2208. https://doi.org/10.3892/ol.2015.3611
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