The effects of Livin shRNA on the response to cisplatin in HepG2 cells

  • Authors:
    • Fangfeng Liu
    • Hong Chang
    • Wei Xu
    • Yunpeng Zhai
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  • Published online on: August 25, 2015     https://doi.org/10.3892/ol.2015.3629
  • Pages: 2957-2961
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Abstract

Hepatocellular carcinoma is a lethal malignancy with poor prognosis, partially due to tumor metastasis, recurrence and resistance to chemo‑ or radio‑therapy. Cisplatin can inhibit cancer cell DNA replication, and is widely used in the clinical treatment of tumors. The present study aimed to generate eukaryotic expression vectors for Livin shRNA and to examine the effects of Livin shRNA on the chemosensitivity of HepG2 hepatocellular carcinoma cells. Eukaryotic expression vectors for Livin shRNA (pSD11‑U6/Neo/GFP/Livin) were designed and constructed. The HepG2 hepatocellular carcinoma cell line was transfected with this vector using the liposome method. The expression levels of Livin mRNA and protein were measured by quantitative polymerase chain reaction and western blot analysis. The rate of cell growth inhibition was measured using MTT assay following treatment of the cells with cisplatin (2.0 mg/l). DNA sequencing confirmed that the construction of the eukaryotic expression vector for Livin shRNA had been successful. Transfection of these vectors into HepG2 cells led to a significant reduction in the expression levels of Livin mRNA and protein (P<0.05). Cisplatin treatment was associated with significantly higher rates of cell growth inhibition in HepG2 cells transfected with Livin shRNA compared with those that were not transfected (P<0.05). The vectors constructed in the present study produced effective inhibition of the Livin gene in HepG2 cells and increased the chemosensitivity of hepatocellular carcinoma cells.
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November-2015
Volume 10 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu F, Chang H, Xu W and Zhai Y: The effects of Livin shRNA on the response to cisplatin in HepG2 cells. Oncol Lett 10: 2957-2961, 2015
APA
Liu, F., Chang, H., Xu, W., & Zhai, Y. (2015). The effects of Livin shRNA on the response to cisplatin in HepG2 cells. Oncology Letters, 10, 2957-2961. https://doi.org/10.3892/ol.2015.3629
MLA
Liu, F., Chang, H., Xu, W., Zhai, Y."The effects of Livin shRNA on the response to cisplatin in HepG2 cells". Oncology Letters 10.5 (2015): 2957-2961.
Chicago
Liu, F., Chang, H., Xu, W., Zhai, Y."The effects of Livin shRNA on the response to cisplatin in HepG2 cells". Oncology Letters 10, no. 5 (2015): 2957-2961. https://doi.org/10.3892/ol.2015.3629