miR-128 downregulation promotes growth and metastasis of bladder cancer cells and involves VEGF-C upregulation

Zhou,Xu; Qi,Lin; Tong,Shiyu; Cui,Yu; Chen,Jinbo; Huang,Tianxiang; Chen,Zhi; Zu,Xiong-Bing

doi:10.3892/ol.2015.3689

miR-128 downregulation promotes growth and metastasis of bladder cancer cells and involves VEGF-C upregulation

Authors:
- Xu Zhou
- Lin Qi
- Shiyu Tong
- Yu Cui
- Jinbo Chen
- Tianxiang Huang
- Zhi Chen
- Xiong-Bing Zu
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Affiliations: Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan 430121, P.R. China, Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 430121, P.R. China
Published online on: September 9, 2015 https://doi.org/10.3892/ol.2015.3689
Pages: 3183-3190

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Abstract

MicroRNA-128 (miR-128) serves an important role in regulating growth, invasiveness, stem cell‑like traits, differentiation and apoptosis of different types of tumor cells. Vascular endothelial growth factor-C (VEGF-C) has been associated with angiogenesis, lymphangiogenesis and regional lymph node metastasis and has previously been reported to have an anti‑apoptotic and proliferative role in bladder cancer (BC). To investigate the regulation of miR‑128 on VEGF‑C expression and their effects on proliferation and metastasis of bladder cancer, T24 and 5637 BC cells were transfected with pre‑miR‑128, anti‑miR‑128 and their respective negative control. miR‑128 was downregulated in BC tissues and cell lines, while the expression levels of VEGF‑C were upregulated. The present results indicated that miR‑128 negatively regulated VEGF‑C expression in BC T24 and 5637 BC cells. VEGF‑C is a direct target of miR‑128 in BC cells. Overexpression of miR‑128 inhibited cell proliferation, migration and invasion. Knockdown of miR‑128 promoted proliferation, migration and invasion in BC cells. Therefore, downregulation mediated malignant progression of BC may be partly attributed to increased VEGF‑C expression. Consequently, the findings of the present study provide a molecular basis for the role of miR-128/VEGF-C in the progression of human BC and indicate a novel target for treatment of BC.

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