Expression and clinical significance of Sirt1 in colorectal cancer

  • Authors:
    • Deng‑Feng Yu
    • Su‑Juan Jiang
    • Zhi‑Peng Pan
    • Wei‑Dong Cheng
    • Wen‑Jun Zhang
    • Xiao‑Kun Yao
    • Yu‑Cheng Li
    • Yong‑Zhi Lun
  • View Affiliations

  • Published online on: December 1, 2015     https://doi.org/10.3892/ol.2015.3982
  • Pages: 1167-1172
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Abstract

The objective of the present study was to examine the expression of Silent information regulator 1 (Sirt1) in colorectal cancer and peritumoral normal mucosa tissue, and therefore analyze the role and molecular mechanism of Sirt1 in the pathogenesis of colorectal cancer. Colorectal cancer tissue specimens were employed as the experimental group, and adjacent normal mucosa tissues >5 cm from tumor lesions were used as the control group. The expression of Sirt1 was detected by the immunohistochemical streptavidin peroxidase detection method in paraffin‑embedded sections, whilst Sirt1 protein expression was examined by western blot analysis in the fresh tissues. Sirt1 protein was primarily expressed in the nuclei of the tumor cells, and positive staining was brownish‑yellow in color. The relative expression quantities of Sirt1 in the peritumoral normal rectal mucosa and rectal carcinoma were 1.15 and 2.62, and the differences between the two groups were statistically significant (P<0.05). The expression level of Sirt1 in colorectal carcinoma was significantly associated with the depth of tumor invasion, differentiation and tumor size (P<0.05). Sirt1 expression was also found to be associated with tumor tissue type, lymph node metastasis, Duke's stage and patient age. These characteristics combined may therefore be used as markers for the early diagnosis of colorectal cancer pathogenesis.
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February-2016
Volume 11 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yu DF, Jiang SJ, Pan ZP, Cheng WD, Zhang WJ, Yao XK, Li YC and Lun YZ: Expression and clinical significance of Sirt1 in colorectal cancer. Oncol Lett 11: 1167-1172, 2016
APA
Yu, D., Jiang, S., Pan, Z., Cheng, W., Zhang, W., Yao, X. ... Lun, Y. (2016). Expression and clinical significance of Sirt1 in colorectal cancer. Oncology Letters, 11, 1167-1172. https://doi.org/10.3892/ol.2015.3982
MLA
Yu, D., Jiang, S., Pan, Z., Cheng, W., Zhang, W., Yao, X., Li, Y., Lun, Y."Expression and clinical significance of Sirt1 in colorectal cancer". Oncology Letters 11.2 (2016): 1167-1172.
Chicago
Yu, D., Jiang, S., Pan, Z., Cheng, W., Zhang, W., Yao, X., Li, Y., Lun, Y."Expression and clinical significance of Sirt1 in colorectal cancer". Oncology Letters 11, no. 2 (2016): 1167-1172. https://doi.org/10.3892/ol.2015.3982