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Article

MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1

Retraction in: /10.3892/ol.2021.12906
  • Authors:
    • Xiumin Zhao
    • Danyang Zhu
    • Cailing Lu
    • Dewen Yan
    • Lifeng Li
    • Zhoufang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The First People's Hospital of Taizhou City, Taizhou, Zhejiang 318020, P.R. China
  • Pages: 1207-1212
    |
    Published online on: December 3, 2015
       https://doi.org/10.3892/ol.2015.4001
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Abstract

MicroRNAs (miRs) have been demonstrated to serve important roles in the development and progression of human cancer, primarily through the direct targeting of oncogenes or tumor suppressors. It has been previously suggested that miR‑126 may be associated with endometrial cancer (EC). However, the exact role of miR‑126 in the migration and invasion of EC cells has not yet been studied. The present study demonstrated that the expression of miR‑126 was significantly decreased in EC tissues when compared with matched normal adjacent tissues. The current study reverse transcription-quantitative polymerase chain reaction was performed in order to examine the expression level of miR-126. Wound healing and transwell assays were used to examine cell migration and invasion. A luciferase reporter assay was used to determine the targeting relationship and western blotting assay was performed to detect the protein expression. Furthermore, the overexpression of miR‑126 significantly inhibited EC SKOV3 cell migration and invasion. Molecular mechanism investigation established that insulin receptor substrate 1 (IRS1) functioned as a direct miR‑126 target, and its expression was negatively regulated by miR‑126 at a post‑transcriptional level in the SKOV3 cells. Additionally, the overexpression of IRS1 reversed the inhibitory effect of miR‑126 overexpression on SKOV3 cell migration and invasion. In conclusion, the current study demonstrated that miR‑126 inhibited EC cell migration and invasion, at least partially through the direct targeting of IRS1, suggesting that miR‑126 may aid the treatment of EC metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao X, Zhu D, Lu C, Yan D, Li L and Chen Z: MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1 Retraction in /10.3892/ol.2021.12906. Oncol Lett 11: 1207-1212, 2016.
APA
Zhao, X., Zhu, D., Lu, C., Yan, D., Li, L., & Chen, Z. (2016). MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1 Retraction in /10.3892/ol.2021.12906. Oncology Letters, 11, 1207-1212. https://doi.org/10.3892/ol.2015.4001
MLA
Zhao, X., Zhu, D., Lu, C., Yan, D., Li, L., Chen, Z."MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1 Retraction in /10.3892/ol.2021.12906". Oncology Letters 11.2 (2016): 1207-1212.
Chicago
Zhao, X., Zhu, D., Lu, C., Yan, D., Li, L., Chen, Z."MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1 Retraction in /10.3892/ol.2021.12906". Oncology Letters 11, no. 2 (2016): 1207-1212. https://doi.org/10.3892/ol.2015.4001
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao X, Zhu D, Lu C, Yan D, Li L and Chen Z: MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1 Retraction in /10.3892/ol.2021.12906. Oncol Lett 11: 1207-1212, 2016.
APA
Zhao, X., Zhu, D., Lu, C., Yan, D., Li, L., & Chen, Z. (2016). MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1 Retraction in /10.3892/ol.2021.12906. Oncology Letters, 11, 1207-1212. https://doi.org/10.3892/ol.2015.4001
MLA
Zhao, X., Zhu, D., Lu, C., Yan, D., Li, L., Chen, Z."MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1 Retraction in /10.3892/ol.2021.12906". Oncology Letters 11.2 (2016): 1207-1212.
Chicago
Zhao, X., Zhu, D., Lu, C., Yan, D., Li, L., Chen, Z."MicroRNA-126 inhibits the migration and invasion of endometrial cancer cells by targeting insulin receptor substrate 1 Retraction in /10.3892/ol.2021.12906". Oncology Letters 11, no. 2 (2016): 1207-1212. https://doi.org/10.3892/ol.2015.4001
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