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Article

Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence

  • Authors:
    • Jian‑Zhi Gao
    • Jia Li
    • Jing‑Li Du
    • Xiao‑Lei Li
  • View Affiliations / Copyright

    Affiliations: Department of Basic Medical Sciences, Xinxiang Medical College, Xinxiang, Henan 453003, P.R. China, Department of Pathology, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China, Department of Molecular Biology, Institute of Basic Medicine, Chinese People's Liberation Army General Hospital, Beijing 100853, P.R. China
  • Pages: 1791-1798
    |
    Published online on: January 19, 2016
       https://doi.org/10.3892/ol.2016.4130
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Abstract

The present study aimed to investigate the expression level of HOX transcript antisense RNA (HOTAIR) in hepatocellular carcinoma (HCC) and its association with various clinicopathological characteristics, and to further explore the molecular mechanisms of HOTAIR function in HCC. Quantitative reverse transcription‑polymerase chain reaction (RT‑PCR) was used to detect the expression level of HOTAIR in 60 paired fresh HCC samples and adjacent normal liver tissue samples. The association between HOTAIR expression and clinicopathological parameters was analyzed. Lentivirus‑mediated HOTAIR‑specific small hairpin RNA vectors were transfected into HepG2 cells. Cell proliferation and invasion in vitro were examined by MTT and Transwell assays, respectively. A xenograft model was used to analyze the tumorigenesis of liver cancer cells in vivo. In addition, semi‑quantitative RT‑PCR was used to detect the expression level of Wnt/β-catenin signaling molecules under the condition of HOTAIR inhibition. The results revealed that the expression level of HOTAIR in HCC tissues was higher than that in adjacent non‑cancerous tissues. HOTAIR expression was significantly associated with poor tumor differentiation (P=0.002), metastasis (P=0.002) and early recurrence (P=0.001). In vitro, the inhibition of HOTAIR in liver cancer cells resulted in the suppression of cell proliferation and invasion. HOTAIR depletion significantly inhibited the rate of growth of liver cancer cells in vivo. Furthermore, the expression levels of Wnt and β‑catenin were downregulated when HOTAIR expression was suppressed. In conclusion, HOTAIR is important in the progression and recurrence of HCC, partly through the regulation of the Wnt/β‑catenin signaling pathway. Targeting HOTAIR may be a novel therapeutic strategy for HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Gao JZ, Li J, Du JL and Li XL: Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence. Oncol Lett 11: 1791-1798, 2016.
APA
Gao, J., Li, J., Du, J., & Li, X. (2016). Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence. Oncology Letters, 11, 1791-1798. https://doi.org/10.3892/ol.2016.4130
MLA
Gao, J., Li, J., Du, J., Li, X."Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence". Oncology Letters 11.3 (2016): 1791-1798.
Chicago
Gao, J., Li, J., Du, J., Li, X."Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence". Oncology Letters 11, no. 3 (2016): 1791-1798. https://doi.org/10.3892/ol.2016.4130
Copy and paste a formatted citation
x
Spandidos Publications style
Gao JZ, Li J, Du JL and Li XL: Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence. Oncol Lett 11: 1791-1798, 2016.
APA
Gao, J., Li, J., Du, J., & Li, X. (2016). Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence. Oncology Letters, 11, 1791-1798. https://doi.org/10.3892/ol.2016.4130
MLA
Gao, J., Li, J., Du, J., Li, X."Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence". Oncology Letters 11.3 (2016): 1791-1798.
Chicago
Gao, J., Li, J., Du, J., Li, X."Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence". Oncology Letters 11, no. 3 (2016): 1791-1798. https://doi.org/10.3892/ol.2016.4130
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