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Article

Effect of CD38 on the multidrug resistance of human chronic myelogenous leukemia K562 cells to doxorubicin

  • Authors:
    • Leman Yalçintepe
    • Emre Halis
    • Sibel Ulku
  • View Affiliations / Copyright

    Affiliations: Department of Biophysics, Istanbul Faculty of Medicine, Istanbul University, Çapa‑Istanbul, Istanbul 34093, Turkey
  • Pages: 2290-2296
    |
    Published online on: January 29, 2016
       https://doi.org/10.3892/ol.2016.4165
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Abstract

Drug resistance is a serious challenge in cancer chemotherapy. Alterations in the intracellular concentration and homeostasis of calcium (Ca2+) may contribute to the development of drug resistance. To investigate the mechanism of drug resistance in leukemia, the present study rendered human chronic myelogenous leukemia K562 cells resistant to the cytotoxic effect of doxorubicin by progressively adapting the sensitive parental K562 cells to doxorubicin. The resulting cells were termed K562/DOX. Subsequently, the expression of two multidrug resistance proteins, P‑glycoprotein (P‑gp) and multidrug resistance protein 1 (MRP1), was analyzed in K562/DOX cells. In addition to P‑gp and MRP1, these cells also expressed cluster of differentiation (CD)38 and its active enzyme adenosine diphosphate (ADP)‑ribosyl cyclase. The present study also demonstrated that K562/DOX cells responded to cyclic ADP‑ribose‑mediated increases in intracellular Ca2+. These data indicate that CD38 may participate in the development of drug resistance to doxorubicin in K562 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Yalçintepe L, Halis E and Ulku S: Effect of CD38 on the multidrug resistance of human chronic myelogenous leukemia K562 cells to doxorubicin. Oncol Lett 11: 2290-2296, 2016.
APA
Yalçintepe, L., Halis, E., & Ulku, S. (2016). Effect of CD38 on the multidrug resistance of human chronic myelogenous leukemia K562 cells to doxorubicin. Oncology Letters, 11, 2290-2296. https://doi.org/10.3892/ol.2016.4165
MLA
Yalçintepe, L., Halis, E., Ulku, S."Effect of CD38 on the multidrug resistance of human chronic myelogenous leukemia K562 cells to doxorubicin". Oncology Letters 11.3 (2016): 2290-2296.
Chicago
Yalçintepe, L., Halis, E., Ulku, S."Effect of CD38 on the multidrug resistance of human chronic myelogenous leukemia K562 cells to doxorubicin". Oncology Letters 11, no. 3 (2016): 2290-2296. https://doi.org/10.3892/ol.2016.4165
Copy and paste a formatted citation
x
Spandidos Publications style
Yalçintepe L, Halis E and Ulku S: Effect of CD38 on the multidrug resistance of human chronic myelogenous leukemia K562 cells to doxorubicin. Oncol Lett 11: 2290-2296, 2016.
APA
Yalçintepe, L., Halis, E., & Ulku, S. (2016). Effect of CD38 on the multidrug resistance of human chronic myelogenous leukemia K562 cells to doxorubicin. Oncology Letters, 11, 2290-2296. https://doi.org/10.3892/ol.2016.4165
MLA
Yalçintepe, L., Halis, E., Ulku, S."Effect of CD38 on the multidrug resistance of human chronic myelogenous leukemia K562 cells to doxorubicin". Oncology Letters 11.3 (2016): 2290-2296.
Chicago
Yalçintepe, L., Halis, E., Ulku, S."Effect of CD38 on the multidrug resistance of human chronic myelogenous leukemia K562 cells to doxorubicin". Oncology Letters 11, no. 3 (2016): 2290-2296. https://doi.org/10.3892/ol.2016.4165
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