Novel possibilities in the study of the salivary proteomic profile using seldi-TOF/MS technology (Review)

  • Authors:
    • Fatima Ardito
    • Donatella Perrone
    • Roberto Cocchi
    • Lucio Lo Russo
    • Alfredo De Lillo
    • Giovanni Giannatempo
    • Lorenzo Lo Muzio
  • View Affiliations

  • Published online on: February 9, 2016     https://doi.org/10.3892/ol.2016.4219
  • Pages: 1967-1972
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Abstract

There is currently an increasing interest in exploring human saliva to identify salivary diagnostic and prognostic biomarkers, since the collection of saliva is rapid, non‑invasive and stress-free. Diagnostic tests on saliva are common and cost-effective, particularly for patients who need to monitor their hormone levels or the effectiveness of undergoing therapies. Furthermore, salivary diagnostics is ideal for surveillance studies and in situations where fast results and inexpensive technologies are required. The most important constituents of saliva are proteins, the expression levels of which may be modified due to variations of the cellular conditions. Therefore, the different profile of proteins detected in saliva, including their absence, presence or altered levels, is a potential biomarker of certain physiological and/or pathological conditions. A promising novel approach to study saliva is the global analysis of salivary proteins using proteomic techniques. In the present study, surface-enhanced laser desorption/ionization-time-of-flight/mass spectrometry (SELDI-TOF/MS), one of the most recent proteomic tools for the identification of novel biomarkers, is reviewed. In addition, the possible use of this technique in salivary proteomic studies is discussed, since SELDI technology combines the precision of matrix-assisted laser desorption/ionization-TOF/MS proteomic analysis and the high-throughput nature of protein array analysis.
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March-2016
Volume 11 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ardito F, Perrone D, Cocchi R, Lo Russo L, De Lillo A, Giannatempo G and Lo Muzio L: Novel possibilities in the study of the salivary proteomic profile using seldi-TOF/MS technology (Review). Oncol Lett 11: 1967-1972, 2016
APA
Ardito, F., Perrone, D., Cocchi, R., Lo Russo, L., De Lillo, A., Giannatempo, G., & Lo Muzio, L. (2016). Novel possibilities in the study of the salivary proteomic profile using seldi-TOF/MS technology (Review). Oncology Letters, 11, 1967-1972. https://doi.org/10.3892/ol.2016.4219
MLA
Ardito, F., Perrone, D., Cocchi, R., Lo Russo, L., De Lillo, A., Giannatempo, G., Lo Muzio, L."Novel possibilities in the study of the salivary proteomic profile using seldi-TOF/MS technology (Review)". Oncology Letters 11.3 (2016): 1967-1972.
Chicago
Ardito, F., Perrone, D., Cocchi, R., Lo Russo, L., De Lillo, A., Giannatempo, G., Lo Muzio, L."Novel possibilities in the study of the salivary proteomic profile using seldi-TOF/MS technology (Review)". Oncology Letters 11, no. 3 (2016): 1967-1972. https://doi.org/10.3892/ol.2016.4219