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Article

Intravital imaging of the effects of 5-fluorouracil on the murine liver microenvironment using 2-photon laser scanning microscopy

  • Authors:
    • Masato Okigami
    • Koji Tanaka
    • Yasuhiro Inoue
    • Susumu Saigusa
    • Yoshinaga Okugawa
    • Yuji Toiyama
    • Yasuhiko Mohri
    • Masato Kusunoki
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal and Pediatric Surgery, Division of Reparative Medicine, Institute of Life Sciences, Mie University Graduate School of Medicine, Tsu, Mie 514‑8507, Japan
  • Pages: 2433-2439
    |
    Published online on: February 23, 2016
       https://doi.org/10.3892/ol.2016.4258
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Abstract

5-fluorouracil (5FU) is often used in the treatment of colorectal cancer. 5FU improves the median overall and disease‑free survival rates and reduces recurrence rates in patients who have undergone curative surgical resection. However, in the adjuvant setting, whether 5FU eradicates clinically undetectable micrometastases in target organs such as the liver, or whether 5‑FU inhibits the adhesion of circulating tumor cells has not yet been established. In the present study, 5FU was administered following the inoculation of red fluorescent protein-expressing HT29 cells into green fluorescent protein (GFP)-transgenic nude mice to examine its inhibitory effect. 2-photon laser scanning microscopy was performed at selected time points for time‑series imaging of liver metastasis of GFP‑transgenic mice. The cell number in vessels was quantified to evaluate the response of the tumor microenvironment to chemotherapy. HT29 cells were visualized in hepatic sinusoids at the single‑cell level. A total of 2 hours after the injection (early stage), time‑series imaging revealed that the number of caught tumor cells gradually reduced over time. In the 5FU treatment group, no significant difference was observed in the cell number in the early stage. One week after the injection (late stage), a difference in morphology was observed. The results of the present study indicated that 5FU eradicated clinically undetectable micrometastases in liver tissues by acting as a cytotoxic agent opposed to preventing adhesion. The present study indicated that time‑series intravital 2‑photon laser scanning microscopic imaging of metastatic tumor xenografts may facilitate the screening and evaluation of novel chemotherapeutic agents with less interindividual variability.
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Copy and paste a formatted citation
Spandidos Publications style
Okigami M, Tanaka K, Inoue Y, Saigusa S, Okugawa Y, Toiyama Y, Mohri Y and Kusunoki M: Intravital imaging of the effects of 5-fluorouracil on the murine liver microenvironment using 2-photon laser scanning microscopy. Oncol Lett 11: 2433-2439, 2016.
APA
Okigami, M., Tanaka, K., Inoue, Y., Saigusa, S., Okugawa, Y., Toiyama, Y. ... Kusunoki, M. (2016). Intravital imaging of the effects of 5-fluorouracil on the murine liver microenvironment using 2-photon laser scanning microscopy. Oncology Letters, 11, 2433-2439. https://doi.org/10.3892/ol.2016.4258
MLA
Okigami, M., Tanaka, K., Inoue, Y., Saigusa, S., Okugawa, Y., Toiyama, Y., Mohri, Y., Kusunoki, M."Intravital imaging of the effects of 5-fluorouracil on the murine liver microenvironment using 2-photon laser scanning microscopy". Oncology Letters 11.4 (2016): 2433-2439.
Chicago
Okigami, M., Tanaka, K., Inoue, Y., Saigusa, S., Okugawa, Y., Toiyama, Y., Mohri, Y., Kusunoki, M."Intravital imaging of the effects of 5-fluorouracil on the murine liver microenvironment using 2-photon laser scanning microscopy". Oncology Letters 11, no. 4 (2016): 2433-2439. https://doi.org/10.3892/ol.2016.4258
Copy and paste a formatted citation
x
Spandidos Publications style
Okigami M, Tanaka K, Inoue Y, Saigusa S, Okugawa Y, Toiyama Y, Mohri Y and Kusunoki M: Intravital imaging of the effects of 5-fluorouracil on the murine liver microenvironment using 2-photon laser scanning microscopy. Oncol Lett 11: 2433-2439, 2016.
APA
Okigami, M., Tanaka, K., Inoue, Y., Saigusa, S., Okugawa, Y., Toiyama, Y. ... Kusunoki, M. (2016). Intravital imaging of the effects of 5-fluorouracil on the murine liver microenvironment using 2-photon laser scanning microscopy. Oncology Letters, 11, 2433-2439. https://doi.org/10.3892/ol.2016.4258
MLA
Okigami, M., Tanaka, K., Inoue, Y., Saigusa, S., Okugawa, Y., Toiyama, Y., Mohri, Y., Kusunoki, M."Intravital imaging of the effects of 5-fluorouracil on the murine liver microenvironment using 2-photon laser scanning microscopy". Oncology Letters 11.4 (2016): 2433-2439.
Chicago
Okigami, M., Tanaka, K., Inoue, Y., Saigusa, S., Okugawa, Y., Toiyama, Y., Mohri, Y., Kusunoki, M."Intravital imaging of the effects of 5-fluorouracil on the murine liver microenvironment using 2-photon laser scanning microscopy". Oncology Letters 11, no. 4 (2016): 2433-2439. https://doi.org/10.3892/ol.2016.4258
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