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Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes

  • Authors:
    • Kai Ding
    • Rong Fu
    • Hui Liu
    • Deepak Anil Nachnani
    • Zong‑Hong Shao
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, General Hospital of Tianjin Medical University, Tianjin 300052, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2347-2352
    |
    Published online on: February 23, 2016
       https://doi.org/10.3892/ol.2016.4259
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Abstract

Thrombocytopenia is a common, often fatal complication experienced by patients with myelodysplastic syndromes (MDS). 5-aza-2'‑deoxycytidine (decitabine) has been used to treat MDS patients with thrombocytopenia with a response rate of 45‑50%. However, the mechanism of its effects on megakaryocytes remains unclear. In the present study, the effect of decitabine on megakaryocyte maturation was investigated. A total of 20 MDS patients diagnosed with thrombocytopenia were enrolled, including 16 refractory anemia with excess blasts (RAEB)‑1 patients and 4 RAEB‑2 patients], in addition to 20 leukemia patients that had achieved complete remission and 20 healthy donors. Overall, 65% of MDS patients exhibited a response to decitabine, with an increase in platelet count identified in 80% of patients. In the MDS group, the mean platelet count was significantly increased following one cycle of decitabine chemotherapy (36.85±24.54 vs. 84.90±61; P=0.001); however, no significant difference in megakaryocyte number was identified prior to and following treatment. Additionally, bone marrow mononuclear cells of the MDS patients were cultured in vitro with various concentrations of decitabine (0.0, 2.0, 2.5, 3.0 µM), and cluster of differentiation (CD)41 levels were examined via flow cytometry. The MDS and normal control groups exhibited the highest levels of CD41 expression following treatment with 2.0 µM decitabine (mean fluorescence intensity, 294.07±47.34 and 258.95±28.05, respectively). In conclusion, these results indicate that the DNA‑hypomethylating agent, decitabine, may induce the differentiation and maturation of myelodysplastic megakaryocytes in MDS patients, even at low concentrations. Thus, the repeated administration of decitabine at lower doses in MDS patients may be useful in clinical practice, and may lead to the development of alternative treatments for other diseases of abnormal megakaryocyte differentiation, such as idiopathic thrombocytopenic purpura, however, future studies are required to investigate this.
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Copy and paste a formatted citation
Spandidos Publications style
Ding K, Fu R, Liu H, Nachnani DA and Shao ZH: Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes. Oncol Lett 11: 2347-2352, 2016.
APA
Ding, K., Fu, R., Liu, H., Nachnani, D.A., & Shao, Z. (2016). Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes. Oncology Letters, 11, 2347-2352. https://doi.org/10.3892/ol.2016.4259
MLA
Ding, K., Fu, R., Liu, H., Nachnani, D. A., Shao, Z."Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes". Oncology Letters 11.4 (2016): 2347-2352.
Chicago
Ding, K., Fu, R., Liu, H., Nachnani, D. A., Shao, Z."Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes". Oncology Letters 11, no. 4 (2016): 2347-2352. https://doi.org/10.3892/ol.2016.4259
Copy and paste a formatted citation
x
Spandidos Publications style
Ding K, Fu R, Liu H, Nachnani DA and Shao ZH: Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes. Oncol Lett 11: 2347-2352, 2016.
APA
Ding, K., Fu, R., Liu, H., Nachnani, D.A., & Shao, Z. (2016). Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes. Oncology Letters, 11, 2347-2352. https://doi.org/10.3892/ol.2016.4259
MLA
Ding, K., Fu, R., Liu, H., Nachnani, D. A., Shao, Z."Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes". Oncology Letters 11.4 (2016): 2347-2352.
Chicago
Ding, K., Fu, R., Liu, H., Nachnani, D. A., Shao, Z."Effects of decitabine on megakaryocyte maturation in patients with myelodysplastic syndromes". Oncology Letters 11, no. 4 (2016): 2347-2352. https://doi.org/10.3892/ol.2016.4259
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