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Article

CDH1 promoter methylation correlates with decreased gene expression and poor prognosis in patients with breast cancer

  • Authors:
    • Jian Liu
    • Xin Sun
    • Sida Qin
    • Huangzhen Wang
    • Ning Du
    • Yanbo Li
    • Yamei Pang
    • Cuicui Wang
    • Chongwen Xu
    • Hong Ren
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China, Department of Neurology, The First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China, Department of Respiration, The First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China, Department of Hematology, Zoucheng People's Hospital, Zoucheng, Shandong 273500, P.R. China
  • Pages: 2635-2643
    |
    Published online on: February 24, 2016
       https://doi.org/10.3892/ol.2016.4274
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Abstract

The E-cadherin gene (CDH1) is associated with poor prognosis and metastasis in patients with breast cancer, and methylation of its promoter is correlated with decreased gene expression. However, there is currently no direct evidence that CDH1 promoter methylation indicates poor prognosis in patients with breast cancer. In the present study, methylation‑specific polymerase chain reaction (PCR) was applied to detect the methylation status of the CDH1 promoter in 137 primary breast cancer, 85 matched normal breast tissue and 13 lung metastasis specimens. Reverse transcription‑quantitative PCR was used to assess the relative expression levels of CDH1 mRNA, and correlation analysis between CDH1 methylation status, and gene expression, clinicopathological characteristics and patient survival was performed. Methylation of CDH1 was identified in 40.9% (56/137) of primary breast cancer specimens, 61.5% (8/13) of lung metastasis specimens and none of the matched normal breast specimens. The downregulation of CDH1 mRNA and E‑cadherin protein expression were identified to be significantly correlated with CDH1 methylation (P<0.05). In addition, CDH1 methylation was significantly associated with lymph node metastasis and estrogen receptor status of patients (P<0.05). In univariate analyses, patients with CDH1 methylation exhibited poor overall survival (OS) and disease‑free survival (DFS; P<0.05). Furthermore, multivariate analyses revealed that CDH1 methylation was an independent prognostic factor predicting poor OS (HR, 1.737; 95% CI, 0.957‑3.766; P=0.041) and DFS (HR, 2.018; 95% CI, 2.057‑3.845; P=0.033) in patients with breast cancer. Therefore, the present study suggests that CDH1 promoter methylation may be correlated with breast carcinogenesis and indicates poor prognosis in patients with breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Sun X, Qin S, Wang H, Du N, Li Y, Pang Y, Wang C, Xu C, Ren H, Ren H, et al: CDH1 promoter methylation correlates with decreased gene expression and poor prognosis in patients with breast cancer. Oncol Lett 11: 2635-2643, 2016.
APA
Liu, J., Sun, X., Qin, S., Wang, H., Du, N., Li, Y. ... Ren, H. (2016). CDH1 promoter methylation correlates with decreased gene expression and poor prognosis in patients with breast cancer. Oncology Letters, 11, 2635-2643. https://doi.org/10.3892/ol.2016.4274
MLA
Liu, J., Sun, X., Qin, S., Wang, H., Du, N., Li, Y., Pang, Y., Wang, C., Xu, C., Ren, H."CDH1 promoter methylation correlates with decreased gene expression and poor prognosis in patients with breast cancer". Oncology Letters 11.4 (2016): 2635-2643.
Chicago
Liu, J., Sun, X., Qin, S., Wang, H., Du, N., Li, Y., Pang, Y., Wang, C., Xu, C., Ren, H."CDH1 promoter methylation correlates with decreased gene expression and poor prognosis in patients with breast cancer". Oncology Letters 11, no. 4 (2016): 2635-2643. https://doi.org/10.3892/ol.2016.4274
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Sun X, Qin S, Wang H, Du N, Li Y, Pang Y, Wang C, Xu C, Ren H, Ren H, et al: CDH1 promoter methylation correlates with decreased gene expression and poor prognosis in patients with breast cancer. Oncol Lett 11: 2635-2643, 2016.
APA
Liu, J., Sun, X., Qin, S., Wang, H., Du, N., Li, Y. ... Ren, H. (2016). CDH1 promoter methylation correlates with decreased gene expression and poor prognosis in patients with breast cancer. Oncology Letters, 11, 2635-2643. https://doi.org/10.3892/ol.2016.4274
MLA
Liu, J., Sun, X., Qin, S., Wang, H., Du, N., Li, Y., Pang, Y., Wang, C., Xu, C., Ren, H."CDH1 promoter methylation correlates with decreased gene expression and poor prognosis in patients with breast cancer". Oncology Letters 11.4 (2016): 2635-2643.
Chicago
Liu, J., Sun, X., Qin, S., Wang, H., Du, N., Li, Y., Pang, Y., Wang, C., Xu, C., Ren, H."CDH1 promoter methylation correlates with decreased gene expression and poor prognosis in patients with breast cancer". Oncology Letters 11, no. 4 (2016): 2635-2643. https://doi.org/10.3892/ol.2016.4274
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