Costunolide induces G1/S phase arrest and activates mitochondrial‑mediated apoptotic pathways in SK‑MES 1 human lung squamous carcinoma cells

  • Authors:
    • Peiyan Hua
    • Guangxin Zhang
    • Yifan Zhang
    • Mei Sun
    • Ranji Cui
    • Xin Li
    • Bingjin Li
    • Xingyi Zhang
  • View Affiliations

  • Published online on: March 1, 2016     https://doi.org/10.3892/ol.2016.4295
  • Pages: 2780-2786
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Abstract

Despite the availability of several therapeutic options, a safer and more effective modality strategy is required for the treatment of lung cancer. Costunolide, a sesquiterpene lactone which isolated from the Saussurea lappa, has potent anticancer properties. In the present study, the effects of costunolide on cell viability, the cell cycle and apoptosis in SK‑MES‑1 human lung squamous carcinoma cells were investigated. Costunolide induced morphological changes and inhibited growth of SK‑MES‑1 cells growth. Flow cytometric analysis data demonstrated that costunolide significantly induced apoptosis of SK‑MES‑1 cells and induced cell cycle arrest at G1/S phase in a dose‑dependent manner. Through upregulation in the expression of p53 and Bax, and downregulation in the expression of Bcl‑2 and activation of caspase‑3, costunolide‑induced apoptosis was confirmed by western blot analysis. In addition, the significant loss of mitochondrial membrane potential indicated that costunolide may induce apoptosis via the mitochondria‑dependent pathway in SK‑MES‑1 cells. These results highlight the potential effects of costunolide as an anti‑cancer agent in a human lung squamous carcinoma cell line.
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April-2016
Volume 11 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Hua P, Zhang G, Zhang Y, Sun M, Cui R, Li X, Li B and Zhang X: Costunolide induces G1/S phase arrest and activates mitochondrial‑mediated apoptotic pathways in SK‑MES 1 human lung squamous carcinoma cells. Oncol Lett 11: 2780-2786, 2016
APA
Hua, P., Zhang, G., Zhang, Y., Sun, M., Cui, R., Li, X. ... Zhang, X. (2016). Costunolide induces G1/S phase arrest and activates mitochondrial‑mediated apoptotic pathways in SK‑MES 1 human lung squamous carcinoma cells. Oncology Letters, 11, 2780-2786. https://doi.org/10.3892/ol.2016.4295
MLA
Hua, P., Zhang, G., Zhang, Y., Sun, M., Cui, R., Li, X., Li, B., Zhang, X."Costunolide induces G1/S phase arrest and activates mitochondrial‑mediated apoptotic pathways in SK‑MES 1 human lung squamous carcinoma cells". Oncology Letters 11.4 (2016): 2780-2786.
Chicago
Hua, P., Zhang, G., Zhang, Y., Sun, M., Cui, R., Li, X., Li, B., Zhang, X."Costunolide induces G1/S phase arrest and activates mitochondrial‑mediated apoptotic pathways in SK‑MES 1 human lung squamous carcinoma cells". Oncology Letters 11, no. 4 (2016): 2780-2786. https://doi.org/10.3892/ol.2016.4295