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Article Open Access

PTEN‑mRNA engineered mesenchymal stem cell‑mediated cytotoxic effects on U251 glioma cells

  • Authors:
    • Xing Rong Guo
    • Qin Yong Hu
    • Ya Hong Yuan
    • Xiang Jun Tang
    • Zhuo Shun Yang
    • Dan Dan Zou
    • Liu Jiao Bian
    • Long Jun Dai
    • Dong Sheng Li
  • View Affiliations / Copyright

    Affiliations: Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China, Department of Surgery, University of British Columbia, Vancouver, BC V5Z 1L8, Canada
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2733-2740
    |
    Published online on: March 1, 2016
       https://doi.org/10.3892/ol.2016.4297
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Abstract

Mesenchymal stem cells (MSCs) have been considered to have potential as ideal carriers for the delivery of anticancer agents since the capacity for tumor‑oriented migration and integration was identified. In contrast to DNA‑based vectors, mRNA synthesized in vitro may be readily transfected and is mutagenesis‑free. The present study was performed in order to investigate the effects of phosphatase and tensin homolog (PTEN) mRNA‑engineered MSCs on human glioma U251 cells under indirect co‑culture conditions. PTEN‑bearing mRNA was generated by in vitro transcription and was transfected into MSCs. The expression of PTEN in transfected MSCs was detected by immunoblotting, and the migration ability of MSCs following PTEN‑bearing mRNA transfection was verified using Transwell co‑cultures. The indirect co‑culture was used to determine the effects of PTEN‑engineered MSCs on the viability of U251 glioma cells by luminescence and fluorescence microscopy. The synthesized PTEN mRNA was expressed in MSCs, and the expression was highest at 24 h subsequent to transfection. An enhanced migration rate was observed in MSCs transfected with PTEN mRNA compared with non‑transfected MSCs (P<0.05). A significant inhibition of U251 cells was observed when the cells were cultured with conditioned medium from PTEN mRNA‑engineered MSCs (P<0.05). The results suggested that anticancer gene‑bearing mRNA synthesized in vitro is capable of being applied to a MSC‑mediated anticancer strategy for the treatment of glioblastoma patients.
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Copy and paste a formatted citation
Spandidos Publications style
Guo XR, Hu QY, Yuan YH, Tang XJ, Yang ZS, Zou DD, Bian LJ, Dai LJ and Li DS: PTEN‑mRNA engineered mesenchymal stem cell‑mediated cytotoxic effects on U251 glioma cells. Oncol Lett 11: 2733-2740, 2016.
APA
Guo, X.R., Hu, Q.Y., Yuan, Y.H., Tang, X.J., Yang, Z.S., Zou, D.D. ... Li, D.S. (2016). PTEN‑mRNA engineered mesenchymal stem cell‑mediated cytotoxic effects on U251 glioma cells. Oncology Letters, 11, 2733-2740. https://doi.org/10.3892/ol.2016.4297
MLA
Guo, X. R., Hu, Q. Y., Yuan, Y. H., Tang, X. J., Yang, Z. S., Zou, D. D., Bian, L. J., Dai, L. J., Li, D. S."PTEN‑mRNA engineered mesenchymal stem cell‑mediated cytotoxic effects on U251 glioma cells". Oncology Letters 11.4 (2016): 2733-2740.
Chicago
Guo, X. R., Hu, Q. Y., Yuan, Y. H., Tang, X. J., Yang, Z. S., Zou, D. D., Bian, L. J., Dai, L. J., Li, D. S."PTEN‑mRNA engineered mesenchymal stem cell‑mediated cytotoxic effects on U251 glioma cells". Oncology Letters 11, no. 4 (2016): 2733-2740. https://doi.org/10.3892/ol.2016.4297
Copy and paste a formatted citation
x
Spandidos Publications style
Guo XR, Hu QY, Yuan YH, Tang XJ, Yang ZS, Zou DD, Bian LJ, Dai LJ and Li DS: PTEN‑mRNA engineered mesenchymal stem cell‑mediated cytotoxic effects on U251 glioma cells. Oncol Lett 11: 2733-2740, 2016.
APA
Guo, X.R., Hu, Q.Y., Yuan, Y.H., Tang, X.J., Yang, Z.S., Zou, D.D. ... Li, D.S. (2016). PTEN‑mRNA engineered mesenchymal stem cell‑mediated cytotoxic effects on U251 glioma cells. Oncology Letters, 11, 2733-2740. https://doi.org/10.3892/ol.2016.4297
MLA
Guo, X. R., Hu, Q. Y., Yuan, Y. H., Tang, X. J., Yang, Z. S., Zou, D. D., Bian, L. J., Dai, L. J., Li, D. S."PTEN‑mRNA engineered mesenchymal stem cell‑mediated cytotoxic effects on U251 glioma cells". Oncology Letters 11.4 (2016): 2733-2740.
Chicago
Guo, X. R., Hu, Q. Y., Yuan, Y. H., Tang, X. J., Yang, Z. S., Zou, D. D., Bian, L. J., Dai, L. J., Li, D. S."PTEN‑mRNA engineered mesenchymal stem cell‑mediated cytotoxic effects on U251 glioma cells". Oncology Letters 11, no. 4 (2016): 2733-2740. https://doi.org/10.3892/ol.2016.4297
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