Open Access

miR-33a is downregulated in melanoma cells and modulates cell proliferation by targeting PCTAIRE1

  • Authors:
    • Fangzhen Tian
    • Hongtu Wei
    • Hua Tian
    • Ying Qiu
    • Jian Xu
  • View Affiliations

  • Published online on: March 9, 2016     https://doi.org/10.3892/ol.2016.4321
  • Pages: 2741-2746
  • Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNA-33a (miR-33a) was previously identified as a lipid regulator that controls the cellular balance between cholesterol and fatty acid metabolism. However, its role in tumor progression is largely unknown. The present study identified that miR-33a acts as a tumor suppressor in melanoma cells. The present study revealed that miR-33a was downregulated in melanoma cells compared with melanocytes. Overexpression of miR-33a suppressed the colony formation of human melanoma SK-MEL-1 and WM-115 cells. Furthermore, a bromodeoxyuridine incorporation assay and anaphase analysis revealed that miR‑33a inhibits melanoma cell proliferation. miR‑33a overexpression inhibited p27 phosphorylation and upregulated p27 expression. Additionally, the present study demonstrated that PCTAIRE1 was a direct target of miR-33a; miR-33a overexpression suppressed the luciferase activity of a reporter construct containing a 3'-untranslated region of PCTAIRE1 and downregulated PCTAIRE1 in melanoma cells. An overexpression of PCTAIRE1 reversed the miR-33a-induced p27 accumulation and tumor suppressive effects. In summary, the present findings offer novel mechanistic insights into miR-33a and its downstream target in melanoma cells.
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April-2016
Volume 11 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Tian F, Wei H, Tian H, Qiu Y and Xu J: miR-33a is downregulated in melanoma cells and modulates cell proliferation by targeting PCTAIRE1. Oncol Lett 11: 2741-2746, 2016
APA
Tian, F., Wei, H., Tian, H., Qiu, Y., & Xu, J. (2016). miR-33a is downregulated in melanoma cells and modulates cell proliferation by targeting PCTAIRE1. Oncology Letters, 11, 2741-2746. https://doi.org/10.3892/ol.2016.4321
MLA
Tian, F., Wei, H., Tian, H., Qiu, Y., Xu, J."miR-33a is downregulated in melanoma cells and modulates cell proliferation by targeting PCTAIRE1". Oncology Letters 11.4 (2016): 2741-2746.
Chicago
Tian, F., Wei, H., Tian, H., Qiu, Y., Xu, J."miR-33a is downregulated in melanoma cells and modulates cell proliferation by targeting PCTAIRE1". Oncology Letters 11, no. 4 (2016): 2741-2746. https://doi.org/10.3892/ol.2016.4321