Open Access

FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer

  • Authors:
    • Yingying Liu
    • Tingting Xia
    • Chunhui Jin
    • Dongmei Gu
    • Jie Yu
    • Weiqiang Shi
    • Ke Zhang
    • Liping Zhang
    • Jianxin Ye
    • Ling Li
  • View Affiliations

  • Published online on: April 14, 2016     https://doi.org/10.3892/ol.2016.4439
  • Pages: 3693-3701
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The transcription factor forkhead box P3 (FOXP3) is involved in immune cell regulation, and carcinoembryonic antigen‑related cell adhesion molecule 6 (CEACAM6) is an adhesion molecule of the immunoglobulin superfamily. These two genes are associated with cancer progression. In the current study, colon tissue specimens from 78 cases of colon cancer (including 40 of stage I‑II and 38 of stage III‑IV), 30 cases of colonic adenoma and 12 healthy controls were collected from the First Affiliated Hospital of Soochow University between January 2010 and December 2011. The expression of cluster of differentiation (CD) 3, CD4, CD8, CD45RO, CEACAM6 and FOXP3 in colon tissues was examined by immunohistochemical analysis. In addition, a reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) assay, based on SYBR Green I, was used to detect CD3, CD4, CD8, CD45RO, CEACAM6 and FOXP3 mRNA levels in the paraffin block specimens. CD3+, CD8+ and CD45RO+ T cell infiltrations in colonic adenoma were significantly higher than in normal colonic mucosa (P<0.001, P=0.001 and P<0.001, respectively). However, CD3+, CD8+ and CD45RO+ lymphocytes in stage III‑IV colon cancer tissues were lower than in normal control tissues (P=0.015, P=0.002 and P=0.041, respectively); consistently, CD3+, CD4+, CD8+ and CD45RO+ lymphocytes in stage III‑IV tissues were even more markedly lower compared with adenoma (P=0.001, P<0.001, P<0.001 and P<0.001, respectively). Similarly, CD3+, CD8+ and CD45RO+ T cell infiltration was lower in stage I‑II cancer tissues compared with adenoma (P=0.001, P<0.001 and P<0.001). CD3+, CD4+, CD8+ and CD45RO+ T cell infiltrations were also significantly higher in stage I‑II compared with stage III‑IV cancer tissues (P<0.001, P=0.045, P<0.001 and P<0.001, respectively). CEACAM6 was found to gradually increase from normal colon tissue to adenoma and cancer tissue. FOXP3 was expressed more highly in stage I‑II compared with normal tissues (P=0.014), and was even higher in stage III‑IV (P<0.001). These results were verified using RT-qPCR, which yielded almost identical results. In summary, the current study demonstrates that FOXP3, CEACAM6 and T cell infiltration are significantly associated with the occurrence and progression of colon cancer, and that immune reactions vary between different stages of colon cancer development.
View Figures
View References

Related Articles

Journal Cover

June-2016
Volume 11 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Xia T, Jin C, Gu D, Yu J, Shi W, Zhang K, Zhang L, Ye J, Li L, Li L, et al: FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer. Oncol Lett 11: 3693-3701, 2016
APA
Liu, Y., Xia, T., Jin, C., Gu, D., Yu, J., Shi, W. ... Li, L. (2016). FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer. Oncology Letters, 11, 3693-3701. https://doi.org/10.3892/ol.2016.4439
MLA
Liu, Y., Xia, T., Jin, C., Gu, D., Yu, J., Shi, W., Zhang, K., Zhang, L., Ye, J., Li, L."FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer". Oncology Letters 11.6 (2016): 3693-3701.
Chicago
Liu, Y., Xia, T., Jin, C., Gu, D., Yu, J., Shi, W., Zhang, K., Zhang, L., Ye, J., Li, L."FOXP3 and CEACAM6 expression and T cell infiltration in the occurrence and development of colon cancer". Oncology Letters 11, no. 6 (2016): 3693-3701. https://doi.org/10.3892/ol.2016.4439