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Article

miR-20b downregulates polymerases κ and θ in XP-V tumor cells

  • Authors:
    • Jia Guo
    • Zheng Jiang
    • Xiangru Li
    • Xi Wang
    • Yan Xiao
  • View Affiliations / Copyright

    Affiliations: Department of Endodontics, Oral Medical Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Endodontics, Xiamen Stomatological Hospital, Xiamen, Fujian 361004, P.R. China
  • Pages: 3790-3794
    |
    Published online on: April 18, 2016
       https://doi.org/10.3892/ol.2016.4447
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Abstract

XP-V is a subtype of Xeroderma pigmentosum diseases with typical pigmentation and cancers in sun-exposed regions. The present study investigated the role of microRNA-20b (miR-20b) in the imbalance of polymerase expression levels in XP‑V tumor cells. Following software prediction results, certain miRNAs were chosen as candidate regulators for the observed imbalance in polymerases in XP‑V tumor cells. Reverse transcription‑quantitative polymerase chain reaction and western blot were used to test candidate miRNAs for their ability to reduce the expression of these polymerases. A luciferase reporter assay was used to further verify the western blot results. Polymerases κ and θ were expressed at lower levels in XP‑V tumor cells compared to normal control cells. A positive correlation was demonstrated between miR-20b and polymerases κ and θ. It was also demonstrated that a proportion of miRNAs had no effect on polymerases κ and θ, despite the software predicting that these miRNAs would target these two polymerases. Therefore, miR‑20b may be responsible for the low expression levels of polymerase κ and θ in XP‑V tumor cells, which accelerated mismatch in DNA replication repairing.
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Copy and paste a formatted citation
Spandidos Publications style
Guo J, Jiang Z, Li X, Wang X and Xiao Y: miR-20b downregulates polymerases κ and θ in XP-V tumor cells. Oncol Lett 11: 3790-3794, 2016.
APA
Guo, J., Jiang, Z., Li, X., Wang, X., & Xiao, Y. (2016). miR-20b downregulates polymerases κ and θ in XP-V tumor cells. Oncology Letters, 11, 3790-3794. https://doi.org/10.3892/ol.2016.4447
MLA
Guo, J., Jiang, Z., Li, X., Wang, X., Xiao, Y."miR-20b downregulates polymerases κ and θ in XP-V tumor cells". Oncology Letters 11.6 (2016): 3790-3794.
Chicago
Guo, J., Jiang, Z., Li, X., Wang, X., Xiao, Y."miR-20b downregulates polymerases κ and θ in XP-V tumor cells". Oncology Letters 11, no. 6 (2016): 3790-3794. https://doi.org/10.3892/ol.2016.4447
Copy and paste a formatted citation
x
Spandidos Publications style
Guo J, Jiang Z, Li X, Wang X and Xiao Y: miR-20b downregulates polymerases κ and θ in XP-V tumor cells. Oncol Lett 11: 3790-3794, 2016.
APA
Guo, J., Jiang, Z., Li, X., Wang, X., & Xiao, Y. (2016). miR-20b downregulates polymerases κ and θ in XP-V tumor cells. Oncology Letters, 11, 3790-3794. https://doi.org/10.3892/ol.2016.4447
MLA
Guo, J., Jiang, Z., Li, X., Wang, X., Xiao, Y."miR-20b downregulates polymerases κ and θ in XP-V tumor cells". Oncology Letters 11.6 (2016): 3790-3794.
Chicago
Guo, J., Jiang, Z., Li, X., Wang, X., Xiao, Y."miR-20b downregulates polymerases κ and θ in XP-V tumor cells". Oncology Letters 11, no. 6 (2016): 3790-3794. https://doi.org/10.3892/ol.2016.4447
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