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Nucleolar localization of Small G protein RhoA is associated with active RNA synthesis in human carcinoma HEp-2 cells

  • Authors:
    • Yueying Li
    • Yong Hu
    • Lilong Che
    • Junhai Jia
    • Min Chen
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China, Department of Neonatology, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3605-3610
    |
    Published online on: April 18, 2016
       https://doi.org/10.3892/ol.2016.4450
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Abstract

Previous studies have demonstrated that the nuclear localization of ras homolog family member A (RhoA), with prominent concentration in the nucleolus, is a common feature in human cancer tissues and cancer cell lines. Although a previous study has demonstrated that the nuclear translocation of RhoA occurs via active transport, a process that occurs through importin α in a nuclear factor‑κB‑dependent manner, the mechanism, biological function and pathological meaning of the nucleolar residency of RhoA remain to be elucidated. As the cell nucleolus is the site of ribosome biosynthesis, the aim of the present study was to investigate the association between RNA synthesis and the nucleolar localization of RhoA, as well as the molecular mechanisms underlying the residency of RhoA in the nucleolus of HEp‑2 (human larynx epithelial carcinoma) cells. Indirect immunofluorescence microscopy was used to evaluate the subcellular distribution of nuclear RhoA, and immunoblotting analysis was used to determine the total cellular protein level of RhoA. Consistent with the results of previous studies, untreated HEp‑2 cells exhibited bright nucleolar staining, indicating an increased concentration of RhoA in the nucleoli. Treatment with actinomycin D for the inhibition of RNA synthesis caused a redistribution of RhoA from the nucleoli to the nucleoplasm with a speckled staining pattern. Immunoblotting revealed that neither the total cellular amount of RhoA nor the integrity of RhoA was affected by treatment with actinomycin D. In cells that were treated at a decreased concentration (0.05 mg/l) of actinomycin D, the redistribution of RhoA was reversible following the removal of the drug from the culture medium. However, this reversal was not observed at an increased drug concentration (1 mg/l). Overall, to the best of our knowledge, the results of the present study provide the first in situ evidence that the inhibition of RNA synthesis induces a redistribution of nucleolar RhoA to the nucleoplasm, and additionally suggest that the nucleolar residency of RhoA in HEp‑2 cells may be associated with active RNA synthesis.
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1 

Misteli T: Protein dynamics: Implications for nuclear architecture and gene expression. Science. 291:843–847. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Scheer U, Thiry M and Goessens G: Structure, function and assembly of the nucleolus. Trends Cell Biol. 3:236–241. 1993. View Article : Google Scholar : PubMed/NCBI

3 

Bártová E, Horáková AH, Uhlírová R, Raska I, Galiová G, Orlova D and Kozubek S: Structure and epigenetics of nucleoli in comparison with non-nucleolar compartments. J Histochem Cytochem. 58:391–403. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Hernandez-Verdun D: The nucleolus today. J Cell Sci. 99:465–471. 1991.PubMed/NCBI

5 

Spector DL: Macromolecular domains within the cell nucleus. Annu Rev Biochem. 9:265–315. 1993. View Article : Google Scholar

6 

Shaw PJ and Jordan EG: The nucleolus. Annu Rev Cell Dev Biol. 11:93–121. 1995. View Article : Google Scholar : PubMed/NCBI

7 

Scheer U and Hock R: Structure and function of the nucleolus. Curr Opin Cell Biol. 11:385–390. 1999. View Article : Google Scholar : PubMed/NCBI

8 

Muro E, Gébrane-Younís J, Jobart-Malfait A, Louvet E, Roussel P and Hernandez-Verdun D: The traffic of proteins between nucleolar organizer regions and prenucleolar bodies governs the assembly of the nucleolus at exit of mitosis. Nucleus. 1:201–211. 2010. View Article : Google Scholar

9 

Pederson T: The plurifunctional nucleolus. Nuclei Acid Res. 26:3871–3876. 1998. View Article : Google Scholar

10 

Pederson T: The nucleolus. Cold Spring Harb Perspect Biol. 3:a0006382011. View Article : Google Scholar : PubMed/NCBI

11 

Mackay DJ and Hall A: Rho GTPases. J Biol Chem. 273:20685–20688. 1998. View Article : Google Scholar : PubMed/NCBI

12 

Sotiropoulos A, Gineitis D, Copeland J and Treisman R: Signal-regulated activation of serum response factor is mediated by changes in actin dynamics. Cell. 98:159–169. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Kamai T, Yamanishi T, Shirataki H, Takagi K, Asami H, Ito Y and Yoshida K: Overexpression of RhoA, Rac1, and Cdc42 GTPases is associated with progression in testicular cancer. Clin Cancer Res. 10:4799–4805. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Dubash AD, Guilluy C, Srougi MC, Boulter E, Burridge K and García-Mata R: The small GTPase RhoA localizes to the nucleus and is activated by Net1 and DNA damage signals. PloS One. 6:e173802011. View Article : Google Scholar : PubMed/NCBI

15 

Guilluy C, Dubash AD and García-Mata R: Analysis of RhoA and Rho GEF activity in whole-cells and the cell nucleus. Nat Protoc. 6:2050–2060. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Li YY, Chen YC and Xu J: Factors influencing RhoA protein distribution in the nucleus. Mol Med Report. 4:1115–1119. 2011.

17 

Li YY, Chen YC, Tao Y, Xu J and Chen M: RhoA protein is generally distributed in the nuclei of cancer cells. Oncol Rep. 24:1005–1009. 2010.PubMed/NCBI

18 

Xu J, Li Y, Yang X, Chen Y and Chen M: Nuclear translocation of Small G protein RhoA via active transportation in gastric cancer cells. Oncol Rep. 30:1878–1882. 2013.PubMed/NCBI

19 

Perry RP and Kelly DE: Inhibition of RNA sythesis by actinomycin D: Characteristic dose-response of different RNA species. J Cell Physiol. 76:127–139. 1970. View Article : Google Scholar : PubMed/NCBI

20 

Chen FM: Binding specificities of actinomycin D to self-complementary tetranucleotide sequences -XGCY-. Biochemistry. 27:6393–6397. 1988. View Article : Google Scholar : PubMed/NCBI

21 

Biggiogera M, Fakan S, Kaufmann SH, Black A, Shaper JH and Busch H: Simultaneous immunoelectron microscopic visualization of protein B23 and C23 distribution in the HeLa cell nucleolus. J Histochem Cytochem. 37:1371–1374. 1989. View Article : Google Scholar : PubMed/NCBI

22 

Sweet P, Chan PK and Slater LM: Cyclosporin A and verapamil enhancement of daunorubicin-produced nucleolar protein B23 translocation in daunorubicin-resistant and -sensitive human and murine tumor cells. Cancer Res. 49:677–680. 1989.PubMed/NCBI

23 

Yung BY, Bor AM and Chan PK: Short exposure to actinomycin D induced ‘reversible’ translocation of protein B23 as well as ‘reversible’ inhibition of cell growth and RNA synthesis in HeLa cells. Cancer Res. 50:5987–5991. 1990.PubMed/NCBI

24 

Yao Z, Duan S, Hou D, Wang W, Wang G, Liu Y, Wen L and Wu M: B23 acts as a nucleolar stress sensor and promotes cell survival through its dynamic interaction with hnRNPU and hnRNPA1. Oncogene. 29:1821–1834. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Benavente R, Reimer G, Rose KM, Hügle-Dörr B and Scheer U: Nucleolar changes after microinjection of antibodies to RNA polymerase I into the nucleus of mammalian cells. Chromosoma. 97:115–123. 1988. View Article : Google Scholar : PubMed/NCBI

26 

Puvion-Dutilleul F, Mazan S, Nicoloso M, Pichard E, Bachellerie JP and Puvion E: Alteration of nucleolar ultrastructure and ribosome biogenesis by actinomycin D. Implications for U3 snRNP function. Eur J Cell Biol. 58:149–162. 1992.PubMed/NCBI

27 

Rivera-León R and Gerbi SA: Delocalization of some small nucleolar RNPs after actinomycin D treatment to deplete early pre-rRNAs. Chromosoma. 105:506–514. 1997. View Article : Google Scholar : PubMed/NCBI

28 

Chen M and Jiang P: Altered subcellular distribution of nucleolar protein fibrillarin by actinomycin D in HEp-2 cells. Acta Pharmacol Sin. 25:902–906. 2004.PubMed/NCBI

29 

Boisvert FM, van Koningsbruggen S, Navascués J and Lamond AI: The multifunctional nucleolus. Nat Rev Mol Cell Biol. 8:574–585. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Montanaro L, Treré D and Derenzini M: Nucleolus, ribosomes, and cancer. Am J Pathol. 173:301–310. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Hein N, Hannan KM, George AJ, Sanij E and Hannan RD: The nucleolus: An emerging target for cancer therapy. Trends Mol Med. 19:643–654. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Pianese G: Beitrag zur Histologie und Aetiologie der Carcinoma. Histologische und experimentelle Untersuchungen. Beitr Pathol Anat Allg Pathol. 142:1–193. 1896.

33 

Quin JE, Devlin JR, Cameron D, Hannan KM, Pearson RB and Hannan RD: Targeting the nucleolus for cancer intervention. Biochim Biophys Acta. 1842:802–816. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Orgaz JL, Herraiz C and Sanz-Moreno V: Rho GTPases modulate malignant transformation of tumor cells. Small GTPases. 5:e290192014. View Article : Google Scholar : PubMed/NCBI

35 

Pickard AJ and Bierbach U: The cell's nucleolus: An emerging target for chemotherapeutic intervention. Chem Med Chem. 8:1441–1449. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Ochs RL: Methods used to study structure and function of the nucleolus. Methods Cell Biol. 53:303–321. 1998. View Article : Google Scholar : PubMed/NCBI

37 

Xu K and Ludueña RF: Characterization of nuclear betaII-tubulin in tumor cells: A possible novel target for taxol. Cell Motil Cytoskeleton. 53:39–52. 2002. View Article : Google Scholar : PubMed/NCBI

38 

Howard J and Hyman AA: Dynamics and mechanics of the microtubule plus end. Nature. 422:753–758. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Hu Y, Che L, Jia J and Chen M: Nucleolar localization of Small G protein RhoA is associated with active RNA synthesis in human carcinoma HEp-2 cells. Oncol Lett 11: 3605-3610, 2016.
APA
Li, Y., Hu, Y., Che, L., Jia, J., & Chen, M. (2016). Nucleolar localization of Small G protein RhoA is associated with active RNA synthesis in human carcinoma HEp-2 cells. Oncology Letters, 11, 3605-3610. https://doi.org/10.3892/ol.2016.4450
MLA
Li, Y., Hu, Y., Che, L., Jia, J., Chen, M."Nucleolar localization of Small G protein RhoA is associated with active RNA synthesis in human carcinoma HEp-2 cells". Oncology Letters 11.6 (2016): 3605-3610.
Chicago
Li, Y., Hu, Y., Che, L., Jia, J., Chen, M."Nucleolar localization of Small G protein RhoA is associated with active RNA synthesis in human carcinoma HEp-2 cells". Oncology Letters 11, no. 6 (2016): 3605-3610. https://doi.org/10.3892/ol.2016.4450
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Hu Y, Che L, Jia J and Chen M: Nucleolar localization of Small G protein RhoA is associated with active RNA synthesis in human carcinoma HEp-2 cells. Oncol Lett 11: 3605-3610, 2016.
APA
Li, Y., Hu, Y., Che, L., Jia, J., & Chen, M. (2016). Nucleolar localization of Small G protein RhoA is associated with active RNA synthesis in human carcinoma HEp-2 cells. Oncology Letters, 11, 3605-3610. https://doi.org/10.3892/ol.2016.4450
MLA
Li, Y., Hu, Y., Che, L., Jia, J., Chen, M."Nucleolar localization of Small G protein RhoA is associated with active RNA synthesis in human carcinoma HEp-2 cells". Oncology Letters 11.6 (2016): 3605-3610.
Chicago
Li, Y., Hu, Y., Che, L., Jia, J., Chen, M."Nucleolar localization of Small G protein RhoA is associated with active RNA synthesis in human carcinoma HEp-2 cells". Oncology Letters 11, no. 6 (2016): 3605-3610. https://doi.org/10.3892/ol.2016.4450
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