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Article

Downregulating microRNA-144 mediates a metabolic shift in lung cancer cells by regulating Glut1 expression

  • Authors:
    • Min Liu
    • Jun Gao
    • Qin Huang
    • Yanfeng Jin
    • Zhonghua Wei
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China
  • Pages: 3772-3776
    |
    Published online on: April 19, 2016
       https://doi.org/10.3892/ol.2016.4468
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Abstract

Lung cancer is the leading cause of cancer-associated mortality worldwide. Increasing evidence has found that cancer metabolism alternations represent a critical hallmark for lung cancer. There is an urgent requirement to understand and dissect the molecular mechanisms underlying cancer metabolism for lung cancer therapy. It remains largely unknown whether the deregulation of miRNAs contributes to the cancer metabolism. The present study aimed to investigate the role of miR‑144 in lung cancer. Glucose uptake rate and lactate production assays demonstrated that miR‑144 expression is decreased and therefore enhances the aerobic metabolism in lung cancer cells. In addition, western blot analysis revealed that miR-144 performs this function by increasing the expression of glucose transporter 1 (GLUT1), leading to an increase in glucose uptake and lactate production. Furthermore, cell viability assays demonstrated that the altered metabolism induced by miR‑144 results in the rapid growth of cancer cells. In conclusion, these results identify miR‑144 as a molecular switch involved in the orchestration of the Warburg effect in lung cancer cells via targeting the expression of GLUT1.
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1 

Mehan MR, Ayers D, Thirstrup D, Xiong W, Ostroff RM, Brody EN, Walker JJ, Gold L, Jarvis TC, Janjic N, et al: Protein signature of lung cancer tissues. PLoS One. 7:e351572012. View Article : Google Scholar : PubMed/NCBI

2 

Zha W, Cao L, Shen Y and Huang M: Roles of Mir-144-ZFX pathway in growth regulation of non-small-cell lung cancer. PloS One. 8:e741752013. View Article : Google Scholar : PubMed/NCBI

3 

Kroemer G and Pouyssegur J: Tumor cell metabolism: Cancer's Achilles' heel. Cancer Cell. 13:472–482. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Muñoz-Pinedo C, El Mjiyad N and Ricci JE: Cancer metabolism: Current perspectives and future directions. Cell Death Dis. 3:e2482012. View Article : Google Scholar : PubMed/NCBI

5 

Vander Heiden MG, Cantley LC and Thompson CB: Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 324:1029–1033. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Jansson MD and Lund AH: MicroRNA and cancer. Mol Oncol. 6:590–610. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Rottiers V and Näär AM: MicroRNAs in metabolism and metabolic disorders. Nat Rev Mol Cell Biol. 13:239–250. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Rayner KJ, Suárez Y, Dávalos A, Parathath S, Fitzgerald ML, Tamehiro N, Fisher EA, Moore KJ and Fernández-Hernando C: MiR-33 contributes to the regulation of cholesterol homeostasis. Science. 328:1570–1573. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Wilfred BR, Wang WX and Nelson PT: Energizing miRNA research: A review of the role of miRNAs in lipid metabolism, with a prediction that miR-103/107 regulates human metabolic pathways. Mol Genet Metab. 91:209–217. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Namløs HM, Meza-Zepeda LA, Barøy T, Østensen IH, Kresse SH, Kuijjer ML, Serra M, Bürger H, Cleton-Jansen AM and Myklebost O: Modulation of the osteosarcoma expression phenotype by microRNAs. PloS One. 7:e480862012. View Article : Google Scholar : PubMed/NCBI

12 

Guled M, Lahti L, Lindholm PM, Salmenkivi K, Bagwan I, Nicholson AG and Knuutila S: CDKN2A, NF2 and JUN are dysregulated among other genes by miRNAs in malignant mesothelioma-A miRNA microarray analysis. Genes Chromosomes Cancer. 48:615–623. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Akiyoshi S, Fukagawa T, Ueo H, Ishibashi M, Takahashi Y, Fabbri M, Sasako M, Maehara Y, Mimori K and Mori M: Clinical significance of miR-144-ZFX axis in disseminated tumour cells in bone marrow in gastric cancer cases. Br J Cancer. 107:1345–1353. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Kalimutho M, Del Vecchio Blanco G, Di Cecilia S, Sileri P, Cretella M, Pallone F, Federici G and Bernardini S: Differential expression of miR-144* as a novel fecal-based diagnostic marker for colorectal cancer. J Gastroenterol. 46:1391–1402. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Zhang LY, Ho-Fun Lee V, Wong AM, Kwong DL, Zhu YH, Dong SS, Kong KL, Chen J, Tsao SW, Guan XY and Fu L: MicroRNA-144 promotes cell proliferation, migration and invasion in nasopharyngeal carcinoma through repression of PTEN. Carcinogenesis. 34:454–463. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e452001. View Article : Google Scholar : PubMed/NCBI

17 

Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, et al: Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 33:e1792005. View Article : Google Scholar : PubMed/NCBI

18 

Lewis BP, Burge CB and Bartel DP: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 120:15–20. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Betel D, Wilson M, Gabow A, Marks DS and Sander C: The microRNA.org resource: Targets and expression. Nucleic Acids Res. 36(Database issue): D149–D153. 2008.PubMed/NCBI

20 

Sun L, Bian G, Meng Z, Dang G, Shi D and Mi S: MiR-144 inhibits uveal melanoma cell proliferation and invasion by regulating c-Met expression. PLoS One. 10:e01244282015. View Article : Google Scholar : PubMed/NCBI

21 

Chen S, Li P, Li J, Wang Y, Du Y, Chen X, Zang W, Wang H, Chu H, Zhao G and Zhang G: MiR-144 inhibits proliferation and induces apoptosis and autophagy in lung cancer cells by targeting TIGAR. Cell Physiol Biochem. 35:997–1007. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Zhao Y, Butler EB and Tan M: Targeting cellular metabolism to improve cancer therapeutics. Cell Death Dis. 4:e5322013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu M, Gao J, Huang Q, Jin Y and Wei Z: Downregulating microRNA-144 mediates a metabolic shift in lung cancer cells by regulating Glut1 expression. Oncol Lett 11: 3772-3776, 2016.
APA
Liu, M., Gao, J., Huang, Q., Jin, Y., & Wei, Z. (2016). Downregulating microRNA-144 mediates a metabolic shift in lung cancer cells by regulating Glut1 expression. Oncology Letters, 11, 3772-3776. https://doi.org/10.3892/ol.2016.4468
MLA
Liu, M., Gao, J., Huang, Q., Jin, Y., Wei, Z."Downregulating microRNA-144 mediates a metabolic shift in lung cancer cells by regulating Glut1 expression". Oncology Letters 11.6 (2016): 3772-3776.
Chicago
Liu, M., Gao, J., Huang, Q., Jin, Y., Wei, Z."Downregulating microRNA-144 mediates a metabolic shift in lung cancer cells by regulating Glut1 expression". Oncology Letters 11, no. 6 (2016): 3772-3776. https://doi.org/10.3892/ol.2016.4468
Copy and paste a formatted citation
x
Spandidos Publications style
Liu M, Gao J, Huang Q, Jin Y and Wei Z: Downregulating microRNA-144 mediates a metabolic shift in lung cancer cells by regulating Glut1 expression. Oncol Lett 11: 3772-3776, 2016.
APA
Liu, M., Gao, J., Huang, Q., Jin, Y., & Wei, Z. (2016). Downregulating microRNA-144 mediates a metabolic shift in lung cancer cells by regulating Glut1 expression. Oncology Letters, 11, 3772-3776. https://doi.org/10.3892/ol.2016.4468
MLA
Liu, M., Gao, J., Huang, Q., Jin, Y., Wei, Z."Downregulating microRNA-144 mediates a metabolic shift in lung cancer cells by regulating Glut1 expression". Oncology Letters 11.6 (2016): 3772-3776.
Chicago
Liu, M., Gao, J., Huang, Q., Jin, Y., Wei, Z."Downregulating microRNA-144 mediates a metabolic shift in lung cancer cells by regulating Glut1 expression". Oncology Letters 11, no. 6 (2016): 3772-3776. https://doi.org/10.3892/ol.2016.4468
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