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Article

Knockdown of hypoxia inducible factor-2α inhibits cell invasion via the downregulation of MMP-2 expression in breast cancer cells

  • Authors:
    • Na Li
    • Hongxing Wang
    • Jie Zhang
    • Erchen Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Clinical Immunology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
  • Pages: 3743-3748
    |
    Published online on: April 19, 2016
       https://doi.org/10.3892/ol.2016.4471
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Abstract

Hypoxia inducible factors (HIFs) are important regulatory molecules of the intracellular oxygen-signaling pathway. The role of HIF-1α has been confirmed in breast carcinoma; however, little is understood concerning the function of HIF‑2α. The present study treated human breast adenocarcinoma MCF‑7 cells with the HIF activator cobalt chloride, and transfected HIF‑2α small interfering RNAs (siRNAs) into MCF‑7 cells to suppress HIF‑2α expression. The siRNAs significantly reduced the levels of HIF‑2α and matrix metalloproteinase (MMP)‑2 in the treated MCF‑7 cells. An invasion assay demonstrated that the siRNAs targeting HIF‑2α inhibited the invasion potency of the cells. The present study concludes that loss of HIF‑2α may be associated with a decreased risk for the progression of human breast cancer, due to the downregulation of the expression of MMP-2.
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1 

Bohensky J, Terkhorn SP, Freeman TA, Adams CS, Garcia JA, Shapiro IM and Srinivas V: Regulation of autophagy in human and murine cartilage: Hypoxia-inducible factor 2 suppresses chondrocyte autophagy. Arthritis Rheum. 60:1406–1415. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Mole DR, Blancher C, Copley RR, Pollard PJ, Gleadle JM, Ragoussis J and Ratcliffe PJ: Genome-wide association of hypoxia-inducible factor (HIF)-1α and HIF-2α DNA binding with expression profiling of hypoxia-inducible transcripts. J Biol Chem. 284:16767–16775. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Kütscher C, Lampert FM, Kunze M, Markfeld-Erol F, Stark GB and Finkenzeller G: Overexpression of hypoxia-inducible factor-1 alpha improves vasculogenesis-related functions of endothelial progenitor cells. Microvasc Res. 105:85–92. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Rankin EB, Rha J, Unger TL, Wu CH, Shutt HP, Johnson RS, Simon MC, Keith B and Haase VH: Hypoxia-inducible factor-2 regulates vascular tumorigenesis in mice. Oncogene. 27:5354–5358. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Copple BL, Bai S and Moon JO: Hypoxia-inducible factor-dependent production of profibrotic mediators by hypoxic Kupffer cells. Hepatol Res. 40:530–539. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Wykoff CC, Sotiriou C, Cockman ME, Ratcliffe PJ, Maxwell P, Liu E and Harris AL: Gene array of VHL mutation and hypoxia shows novel hypoxia-induced genes and that cyclin D1 is a VHL target gene. Br J Cancer. 90:1235–1243. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Liu W, Xin H, Eckert DT, Brown JA and Gnarra JR: Hypoxia and cell cycle regulation of the von Hippel-Lindau tumor suppressor. Oncogene. 30:21–31. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Zhao S, Jin C, Zhao X, Jin B, Hui L, Zhou W, Niu G and Tao S: Expression and clinical significance of ING4 and HIF-1 alpha in brain astrocytoma. Zhonghua Yi Xue Za Zhi. 95:3533–3536. 2015.(In Chinese). PubMed/NCBI

9 

Burrows N, Babur M, Resch J, Williams KJ and Brabant G: Hypoxia-inducible factor in thyroid carcinoma. J Thyroid Res. 2011:7629052011. View Article : Google Scholar : PubMed/NCBI

10 

Trisciuoglio D, Gabellini C, Desideri M, Ziparo E, Zupi G and Del Bufalo D: Bcl-2 regulates HIF-1α protein stabilization in hypoxic melanoma cells via the molecular chaperone HSP90. PLoS One. 5:e117722010. View Article : Google Scholar : PubMed/NCBI

11 

Mardilovich K and Shaw LM: Hypoxia regulates insulin receptor substrate-2 expression to promote breast carcinoma cell survival and invasion. Cancer Res. 69:8894–8901. 2009.10.1158/0008-5472.CAN-09-1152. View Article : Google Scholar : PubMed/NCBI

12 

Raval RR, Lau KW, Tran MGB, Sowter HM, Mandriota SJ, Li JL, Pugh CW, Maxwell PH, Harris AL and Ratcliffe PJ: Contrasting properties of hypoxia-inducible factor 1 (HIF-1) and HIF-2 in von Hippel-Lindau-associated renal cell carcinoma. Mol Cell Biol. 25:5675–5686. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Mazumdar J, Hickey MM, Pant DK, Durham AC, Sweet-Cordero A, Vachani A, Jacks T, Chodosh LA, Kissil JL, Simon MC and Keith B: HIF-2α deletion promotes Kras-driven lung tumor development. Proc Natl Acad Sci USA. 107:14182–14187. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Florczyk U, Czauderna S, Stachurska A, Tertil M, Nowak W, Kozakowska M, Poellinger L, Jozkowicz A, Loboda A and Dulak J: Opposite effects of HIF-1α and HIF-2α on the regulation of IL-8 expression in endothelial cells. Free Radic Biol Med. 1:1882–1892. 2011. View Article : Google Scholar

15 

Rankin EB, Rha J, Selak MA, Unger TL, Keith B, Liu Q and Haase VH: Hypoxia-inducible factor 2 regulates hepatic lipid metabolism. Mol Cell Biol. 29:4527–4538. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Choi YK, Kim CK, Lee H, Jeoung D, Ha KS, Kwon YG, Kim KW and Kim YM: Carbon monoxide promotes VEGF expression by increasing HIF-1α protein level via two distinct mechanisms, translational activation and stabilization of HIF-1α protein. J Biol Chem. 285:32116–32125. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Kapitsinou PP, Liu Q, Unger TL, Rha J, Davidoff O, Keith B, Epstein JA, Moores SL, Erickson-Miller CL and Haase VH: Hepatic HIF-2 regulates erythropoietic responses to hypoxia in renal anemia. Blood. 116:3039–3048. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Rankin EB, Biju MP, Liu Q, Unger TL, Rha J, Johnson RS, Simon MC, Keith B and Haase VH: Hypoxia-inducible factor-2 (HIF-2) regulates hepatic erythropoietin in vivo. J Clin Invest. 117:1068–1077. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Wei K, Piecewicz SM, McGinnis LM, Taniguchi CM, Wiegand SJ, Anderson K, Chan CW, Mulligan KX, Kuo D, Yuan J, et al: A liver Hif-2α-Irs2 pathway sensitizes hepatic insulin signaling and is modulated by Vegf inhibition. Nat Med. 19:1331–1337. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Qiu Y, Zheng H, Sun LH, Peng K, Xiao WD and Yang H: Hypoxia-inducible factor-1 modulates upregulation of mutT homolog-1 in colorectal cancer. World J Gastroenterol. 21:13447–13456. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Fukasawa T, Enomoto A and Miyagawa K: Serine-Threonine Kinase 38 regulates CDC25A stability and the DNA damage-induced G2/M checkpoint. Cell Signal. 27:1569–1575. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Meade ES, Ma YY and Guller S: Role of hypoxia-inducible transcription factors 1α and 2α in the regulation of plasminogen activator inhibitor-1 expression in a human trophoblast cell line. Placenta. 28:1012–1019. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Li N, Wang HX, Zhang A, Ye YP and He GY: KISS-1 inhibits the proliferation and invasion of gastric carcinoma cells. World J Gastroenterol. 18:1827–1833. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Semenza GL: Defining the role of hypoxia-inducible factor 1 in cancer biology and therapeutics. Oncogene. 29:625–634. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Bensellam M, Duvillié B, Rybachuk G, Laybutt DR, Magnan C, Guiot Y, Pouysségur J and Jonas JC: Glucose-induced O2 consumption activates hypoxia inducible factors 1 and 2 in rat insulin-secreting pancreatic beta-cells. PLoS One. 7:e298072012. View Article : Google Scholar : PubMed/NCBI

26 

Jing SW, Wang YD, Kuroda M, Su JW, Sun GG, Liu Q, Cheng YJ and Yang CR: HIF-1α contributes to hypoxia-induced invasion and metastasis of esophageal carcinoma via inhibiting E-cadherin and promoting MMP-2 expression. Acta Med Okayama. 66:399–407. 2012.PubMed/NCBI

27 

Leonard MO, Howell K, Madden SF, Costello CM, Higgins DG, Taylor CT and McLoughlin P: Hypoxia selectively activates the CREB family of transcription factors in the in vivo lung. Am J Respir Crit Care Med. 178:977–983. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Gordan JD, Bertout JA, Hu CJ, Diehl JA and Simon MC: HIF-2α promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity. Cancer Cell. 11:335–347. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Huang KT, Takano EA, Mikeska T, Byrne DJ, Dobrovic A and Fox SB: Aberrant DNA methylation but not mutation of CITED4 is associated with alteration of HIF-regulated genes in breast cancer. Breast Cancer Res Treat. 130:319–329. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Li N, Wang HX, Qin C, Wang XH and Han FY: Relationship between clinicopathological features and HIF-2α in gastric adenocarcinoma. Genet Mol Res. 14:1404–1413. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Xiao H, Gu Z, Wang G and Zhao T: The possible mechanisms underlying the impairment of HIF-1α pathway signaling in hyperglycemia and the beneficial effects of certain therapies. Int J Med Sci. 10:1412–1421. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Yokoe S, Nakagawa T, Kojima Y, Higuchi K and Asahi M: Indomethacin-induced intestinal epithelial cell damage is mediated by pVHL activation through the degradation of collagen I and HIF-1α. Biochem Biophys Res Commun. 468:671–676. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Zhu P, Ning Y, Yao L, Chen M and Xu C: The proliferation, apoptosis, invasion of endothelial-like epithelial ovarian cancer cells induced by hypoxia. J Exp Clin Cancer Res. 29:1242010. View Article : Google Scholar : PubMed/NCBI

34 

Yoo HI, Moon YH and Kim MS: Effects of CoCl2 on multi-lineage differentiation of C3H/10T1/2 mesenchymal stem cells. Korean J Physiol Pharmacol. 20:53–62. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Wang Q, Peng K and He L: Expression of the HIF-2α in epithelial ovarian cancer and clinical significance. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 39:889–893. 2014.PubMed/NCBI

36 

Anelli V, Gault CR, Cheng AB and Obeid LM: Sphingosine kinase 1 is up-regulated during hypoxia in U87MG glioma cells. Role of hypoxia-inducible factors 1 and 2. J Biol Chem. 283:3365–3375. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li N, Wang H, Zhang J and Zhao E: Knockdown of hypoxia inducible factor-2α inhibits cell invasion via the downregulation of MMP-2 expression in breast cancer cells. Oncol Lett 11: 3743-3748, 2016.
APA
Li, N., Wang, H., Zhang, J., & Zhao, E. (2016). Knockdown of hypoxia inducible factor-2α inhibits cell invasion via the downregulation of MMP-2 expression in breast cancer cells. Oncology Letters, 11, 3743-3748. https://doi.org/10.3892/ol.2016.4471
MLA
Li, N., Wang, H., Zhang, J., Zhao, E."Knockdown of hypoxia inducible factor-2α inhibits cell invasion via the downregulation of MMP-2 expression in breast cancer cells". Oncology Letters 11.6 (2016): 3743-3748.
Chicago
Li, N., Wang, H., Zhang, J., Zhao, E."Knockdown of hypoxia inducible factor-2α inhibits cell invasion via the downregulation of MMP-2 expression in breast cancer cells". Oncology Letters 11, no. 6 (2016): 3743-3748. https://doi.org/10.3892/ol.2016.4471
Copy and paste a formatted citation
x
Spandidos Publications style
Li N, Wang H, Zhang J and Zhao E: Knockdown of hypoxia inducible factor-2α inhibits cell invasion via the downregulation of MMP-2 expression in breast cancer cells. Oncol Lett 11: 3743-3748, 2016.
APA
Li, N., Wang, H., Zhang, J., & Zhao, E. (2016). Knockdown of hypoxia inducible factor-2α inhibits cell invasion via the downregulation of MMP-2 expression in breast cancer cells. Oncology Letters, 11, 3743-3748. https://doi.org/10.3892/ol.2016.4471
MLA
Li, N., Wang, H., Zhang, J., Zhao, E."Knockdown of hypoxia inducible factor-2α inhibits cell invasion via the downregulation of MMP-2 expression in breast cancer cells". Oncology Letters 11.6 (2016): 3743-3748.
Chicago
Li, N., Wang, H., Zhang, J., Zhao, E."Knockdown of hypoxia inducible factor-2α inhibits cell invasion via the downregulation of MMP-2 expression in breast cancer cells". Oncology Letters 11, no. 6 (2016): 3743-3748. https://doi.org/10.3892/ol.2016.4471
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