Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
June-2016 Volume 11 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2016 Volume 11 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Liposome‑mediated transfection of wild‑type P53 DNA into human prostate cancer cells is improved by low‑frequency ultrasound combined with microbubbles

  • Authors:
    • Wen‑Kun Bai
    • Wei Zhang
    • Bing Hu
    • Tao Ying
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasound in Medicine, Shanghai Institute of Ultrasound in Medicine, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, P.R. China
  • Pages: 3829-3834
    |
    Published online on: April 20, 2016
       https://doi.org/10.3892/ol.2016.4477
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Prostate cancer is a common type of cancer in elderly men. The aim of the present study was to evaluate the effects of ultrasound exposure in combination with SonoVue microbubbles on liposome‑mediated transfection of wild‑type P53 genes into human prostate cancer cells. PC‑3 human prostate cancer cells were exposed to ultrasound; duty cycle was controlled at 20% (2 sec on, 8 sec off) for 5 min with and without SonoVue microbubble echo‑contrast agent using a digital sonifier (frequency, 21 kHz; intensity, 46 mW/cm2). The cells were divided into eight groups, as follows: Group A (SonoVue + wild‑type P53), group B (ultrasound + wild‑type P53), group C (SonoVue + ultrasound + wild‑type P53), group D (liposome + wild‑type P53), group E (liposome + SonoVue + wild‑type P53), group F (liposome + wild‑type P53 + ultrasound), group G (liposome + wild‑type P53 + ultrasound + SonoVue) and the control group (wild‑type P53). Following treatment, a hemocytometer was used to measure cell lysis, reverse transcription‑quantitative polymerase chain reaction and western blotting were performed to detect P53 gene transfection efficiency, Cell Counting Kit‑8 was employed to reveal cell proliferation and Annexin V/propidium iodide staining was used to determine cell apoptosis. Cell lysis was minimal in each group. Wild‑type P53 gene and protein expression were significantly increased in the PC‑3 cells in group G compared with the control and all other groups (P<0.01). Cell proliferation was significantly suppressed in group G compared with the control group and all other groups (P<0.01). Cell apoptosis levels in group G were significantly improved compared with the control group and all other groups (P<0.01). Thus, the results of the present study indicate that the use of low‑frequency and low‑energy ultrasound in combination with SonoVue microbubbles may be a potent physical method for increasing liposome gene delivery efficiency.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Xu AH, Hu ZM, Qu JB, Liu SM, Syed AK, Yuan HQ and Lou HX: Cyclic bisbibenzyls induce growth arrest and apoptosis of human prostate cancer PC3 cells. Acta Pharmacol Sin. 31:609–615. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Mangar SA, Huddart RA, Parker CC, Dearnaley DP, Khoo VS and Horwich A: Technological advances in radiotherapy for the treatment of localised prostate cancer. Eur J Cancer. 41:908–921. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Tanaka G, Hirata Y, Goldenberg SL, Bruchovsky N and Aihara K: Mathematical modelling of prostate cancer growth and its application to hormone therapy. Philos Trans A Math Phys Eng Sci. 368:5029–5044. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Lecornet E, Ahmed HU, Moore C and Emberton M: Focal therapy for prostate cancer: A potential strategy to address the problem of overtreatment. Arch Esp Urol. 63:845–852. 2010.PubMed/NCBI

6 

Baumert H: Salvage treatments for prostatic radiation failure. Cancer Radiother. 14:442–445. 2010.(In French). View Article : Google Scholar : PubMed/NCBI

7 

Verma IM and Somia N: Gene therapy-promises, problems and prospects. Nature. 389:239–242. 1997. View Article : Google Scholar : PubMed/NCBI

8 

Wyber JA, Andrews J and D'Emanuele A: The use of sonication for the efficient delivery of plasmid DNA into cells. Pharm Res. 14:750–756. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Whelan J: Electroporation and ultrasound for gene and drug delivery. Drug Delivery Today. 11:585–586. 2002. View Article : Google Scholar

10 

Danialou G, Comtois AS, Dudley RW, Nalbantoglu J, Gilbert R, Karpati G, Jones DH and Petrof BJ: Ultrasound increase plasmid-mediated gene transfer to dystrophic muscles without collateral damage. Mol Ther. 5:687–693. 2002. View Article : Google Scholar

11 

Negishi Y, Omata D, Iijima H, Takabayashi Y, Suzuki K, Endo Y, Suzuki R, Maruyama K, Nomizu M and Aramaki Y: Enhanced laminin-derived peptide AG73-mediated liposomal gene transfer by bubble liposomes and ultrasound. Mol Pharm. 7:217–226. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Audouy SA, de Leij LF, Hoekstra D and Molema G: In vivo characteristics of cationic liposomes as delivery vectors for gene therapy. Pharm Res. 19:1599–1605. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Hirko A, Tang F and Hughes JA: Cationic lipid vectors for plasmid DNA delivery. Curr Med Chem. 10:1185–1193. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Lin VC, Huang CY, Lee YC, Yu CC, Chang TY, Lu TL, Huang SP and Bao BY: Genetic variations in TP53 binding sites are predictors of clinical outcomes in prostate cancer patients. Arch Toxicol. 88:901–911. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Han L, Zhao J, Liu J, Duan XL, Li LH, Wei XF, Wei Y and Liang XJ: A universal gene carrier platform for treatment of human prostatic carcinoma by p53 transfection. Biomaterials. 35:3110–3120. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Wan C, Qian J, Li F and Li H: Ultrasound-targeted microbubble destruction enhances polyethylenimine-mediated gene transfection in vitro in human retinal pigment epithelial cells and in vivo in rat retina. Mol Med Rep. 12:2835–2841. 2015.PubMed/NCBI

17 

Sugano M, Negishi Y, Endo-Takahashi Y, Hamano N, Usui M, Suzuki R, Maruyama K, Aramaki Y and Yamamoto M: Gene delivery to periodontal tissue using Bubble liposomes and ultrasound. J Periodontal Res. 49:398–404. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Chen Z, Xie M, Wang X, Lv Q and Ding S: Efficient gene delivery to myocardium with ultrasound targeted microbubble destruction and polyethylenimine. J Huazhong Univ Sci Technolog Med Sci. 28:613–617. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Zolochevska O, Xia X, Williams BJ, Ramsay A, Li S and Figueiredo ML: Sonoporation delivery of interleukin-27 gene therapy efficiently reduces prostate tumor cell growth in vivo. Hum Gene Ther. 22:1537–1550. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Tachibana K and Tachibana S: Transdermal delivery of insulin by ultrasonic vibration. J Pharm Pharmacol. 43:270–271. 1991. View Article : Google Scholar : PubMed/NCBI

21 

Newman CM and Bettinger T: Gene therapy progress and prospects: Ultrasound for gene transfer. Gene Ther. 14:465–475. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Wang G, Zhuo Z, Xia H, Zhang Y, He Y, Tan W and Gao Y: Investigation into the impact of diagnostic ultrasound with microbubbles on the capillary permeability of rat hepatomas. Ultrasound Med Biol. 39:628–637. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S and Sueishi M: Sonoporation: Gene transfer using ultrasound. World J Methodol. 3:39–44. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Marentis TC, Kusler B, Yaralioglu GG, Liu S, Haeggström EO and Khuri-Yakub BT: Microfluidic sonicator for real-time disruption of eukaryotic cells and bacterial spores for DNA analysis. Ultrasound Med Biol. 31:1265–1277. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Bai WK, Wu ZH, Shen E, Zhang JZ and Hu B: The improvement of liposome-mediated transfection of pEGFP DNA into human prostate cancer cells by combining low-frequency and low-energy ultrasound with microbubbles. Oncol Rep. 27:475–480. 2012.PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Scott SL, Earle JD and Gumerlock PH: Functional p53 increases prostate cancer cell survival after exposure to fractionated doses of ionizing radiation. Cancer Res. 63:7190–7196. 2003.PubMed/NCBI

28 

Sicklick JK, Li YX, Jayaraman A, Kannangai R, Qi Y, Vivekanandan P, Ludlow JW, Owzar K, Chen W, Torbenson MS and Diehl AM: Dysregulation of the Hedgehog pathway in human hepatocarcinogenesis. Carcinogenesis. 27:748–757. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Wang XB, Liu QH, Wang P, Zhang K, Tang W and Wang BL: Enhancement of apoptosis by sonodynamic therapy with protoporphyrin IX in isolate sarcoma 180 cells. Cancer Biother Radiopharm. 23:238–246. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Fang HY, Tsai KC, Cheng WH, Shieh MJ, Lou PJ, Lin WL and Chen WS: The effects of power on-off durations of pulsed ultrasound on the destruction of cancer cells. Int J Hyperthermia. 23:371–380. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Kodama T, Tomita Y, Koshiyama K and Blomley MJ: Transfection effect of microbubbles on cells in superposed ultrasound waves and behavior of cavitation bubble. Ultrasound Med Biol. 32:905–914. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Ecke TH, Schlechte HH, Hübsch A, Lenk SV, Schiemenz K, Rudolph BD and Miller K: TP53 mutation in prostate needle biopsies-comparison with patients follow-up. Anticancer Res. 27:4143–4148. 2007.PubMed/NCBI

33 

Gupta K, Thakur VS, Bhaskaran N, Nawab A, Babcook MA, Jackson MW and Gupta S: Green tea polyphenols induce p53-dependent and p53-independent apoptosis in prostate cancer cells through two distinct mechanisms. PLoS One. 7:e525722012. View Article : Google Scholar : PubMed/NCBI

34 

Li X, Li Y, Hu J, Wang B, Zhao L, Ji K, Guo B, Yin D, Du Y, Kopecko DJ, et al: Plasmid-based E6-specific siRNA and co-expression of wild-type p53 suppresses the growth of cervical cancer in vitro and in vivo. Cancer Lett. 335:242–250. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Wang JF, Wu CJ, Zhang CM, Qiu QY and Zheng M: Ultrasound-mediated microbubble destruction facilitates gene transfection in rat C6 glioma cells. Mol Biol Rep. 36:1263–1267. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Manome Y, Nakayama N, Nakayama K and Furuhata H: Insonation facilitates plasmid DNA transfection into the central nervous system and microbubbles enhance the effect. Ultrasound Med Biol. 31:693–702. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Bai WK, Zhang W, Hu B and Ying T: Liposome‑mediated transfection of wild‑type P53 DNA into human prostate cancer cells is improved by low‑frequency ultrasound combined with microbubbles. Oncol Lett 11: 3829-3834, 2016.
APA
Bai, W., Zhang, W., Hu, B., & Ying, T. (2016). Liposome‑mediated transfection of wild‑type P53 DNA into human prostate cancer cells is improved by low‑frequency ultrasound combined with microbubbles. Oncology Letters, 11, 3829-3834. https://doi.org/10.3892/ol.2016.4477
MLA
Bai, W., Zhang, W., Hu, B., Ying, T."Liposome‑mediated transfection of wild‑type P53 DNA into human prostate cancer cells is improved by low‑frequency ultrasound combined with microbubbles". Oncology Letters 11.6 (2016): 3829-3834.
Chicago
Bai, W., Zhang, W., Hu, B., Ying, T."Liposome‑mediated transfection of wild‑type P53 DNA into human prostate cancer cells is improved by low‑frequency ultrasound combined with microbubbles". Oncology Letters 11, no. 6 (2016): 3829-3834. https://doi.org/10.3892/ol.2016.4477
Copy and paste a formatted citation
x
Spandidos Publications style
Bai WK, Zhang W, Hu B and Ying T: Liposome‑mediated transfection of wild‑type P53 DNA into human prostate cancer cells is improved by low‑frequency ultrasound combined with microbubbles. Oncol Lett 11: 3829-3834, 2016.
APA
Bai, W., Zhang, W., Hu, B., & Ying, T. (2016). Liposome‑mediated transfection of wild‑type P53 DNA into human prostate cancer cells is improved by low‑frequency ultrasound combined with microbubbles. Oncology Letters, 11, 3829-3834. https://doi.org/10.3892/ol.2016.4477
MLA
Bai, W., Zhang, W., Hu, B., Ying, T."Liposome‑mediated transfection of wild‑type P53 DNA into human prostate cancer cells is improved by low‑frequency ultrasound combined with microbubbles". Oncology Letters 11.6 (2016): 3829-3834.
Chicago
Bai, W., Zhang, W., Hu, B., Ying, T."Liposome‑mediated transfection of wild‑type P53 DNA into human prostate cancer cells is improved by low‑frequency ultrasound combined with microbubbles". Oncology Letters 11, no. 6 (2016): 3829-3834. https://doi.org/10.3892/ol.2016.4477
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team