Knockdown of β‑catenin by siRNA influences proliferation, apoptosis and invasion of the colon cancer cell line SW480

Li,Kui; Zhou,Zhong‑Yin; Ji,Pan‑Pan; Luo,He‑Sheng

doi:10.3892/ol.2016.4481

June-2016 Volume 11 Issue 6

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

June-2016 Volume 11 Issue 6

Full Size Image

Article

Knockdown of β‑catenin by siRNA influences proliferation, apoptosis and invasion of the colon cancer cell line SW480

Authors:
- Kui Li
- Zhong‑Yin Zhou
- Pan‑Pan Ji
- He‑Sheng Luo
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, Renmin Hospital, Wuhan University, Wuhan, Hubei 430060, P.R. China
Pages: 3896-3900
|
Published online on: April 20, 2016

https://doi.org/10.3892/ol.2016.4481
Expand metrics +

Abstract

The aim of the present study was to explore the effect of knocking down the expression of β‑catenin by small interference (si)RNA on the activity of the Wnt/β‑catenin signaling pathway, and the proliferation, apoptosis and invasion abilities of the human colon cancer cell line SW480. For that purpose, double‑stranded siRNA targeting β‑catenin (β‑catenin‑siRNA) was synthesized and transfected into SW480 cells. Reverse transcription‑polymerase chain reaction (RT‑PCR) and western blotting were used to detect the messenger (m)RNA and protein levels of β‑catenin in SW480 cells. To detect cell proliferation, 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay was performed, while cell apoptosis and caspase‑3 activity were detected by flow cytometry and caspase‑3 activity assay, respectively. Matrigel invasion assay was performed to detect the influence of siRNA‑mediated gene silencing on the invasion and metastasis of SW480 cells in vitro. The results of RT‑PCR and western blot analysis demonstrated that, compared with the blank control, negative control and liposome groups, β‑catenin‑siRNA transfected SW480 cells had significantly decreased mRNA and protein levels of β‑catenin. In addition, following β‑catenin‑siRNA transfection, the proliferation of SW480 cells was significantly lower than that of the blank control, negative control and liposome groups, while the apoptosis rate increased in β‑catenin‑siRNA transfected cells, compared with the aforementioned groups. Invasion assay showed that, following β‑catenin‑siRNA transfection, the number of SW480 cells infiltrating through the Matrigel membrane was significantly lower than that of the blank control, negative control and liposome groups. Following β‑catenin‑siRNA transfection, the caspase‑3 activity in SW480 cells was lower than that in the blank control, negative control and liposome groups. These results indicate that siRNA‑mediated silencing of β‑catenin could inhibit the proliferation and invasion of SW480 cells and induce apoptosis, thus providing novel potential strategies for the clinical treatment of colon cancer, and may serve as a novel target for cancer therapy.

View Figures

View References

1	Fodde R and Brabletz T: Wnt/beta-catenin signaling in cancer stemness and malignant behavior. Curr Opin Cell Biol. 19:150–158. 2007. View Article : Google Scholar : PubMed/NCBI
2	Ring A, Kim YM and Kahn M: Wnt/catenin signaling in adult stem cell physiology and disease. Stem Cell Rev. 10:512–525. 2014. View Article : Google Scholar : PubMed/NCBI
3	Fan K, Li N, Qi J, Yin P, Zhao C, Wang L, Li Z and Zha X: Wnt/β-catenin signaling induces the transcription of cystathionine-γ-lyase, a stimulator of tumor in colon cancer. Cell Signal. 26:2801–2808. 2014. View Article : Google Scholar : PubMed/NCBI
4	Lazarova DL, Wong T, Chiaro C, Drago E and Bordonaro M: p300 influences butyrate-mediated WNT hyperactivation in colorectal cancer cells. J Cancer. 4:491–501. 2013. View Article : Google Scholar : PubMed/NCBI
5	Kumawat K, Koopmans T and Gosens R: β-catenin as a regulator and therapeutic target for asthmatic airway remodeling. Expert Opin Ther Targets. 18:1023–1034. 2014. View Article : Google Scholar : PubMed/NCBI
6	Denais C and Lammerding J: Nuclear mechanics in cancer. Adv Exp Med Biol. 773:435–470. 2014. View Article : Google Scholar : PubMed/NCBI
7	Tan CW, Gardiner BS, Hirokawa Y, Layton MJ, Smith DW and Burgess AW: Wnt signalling pathway parameters for mammalian cells. PLoS One. 7:e318822012. View Article : Google Scholar : PubMed/NCBI
8	Paul S and Dey A: Wnt signaling and cancer development: Therapeutic implication. Neoplasma. 55:165–176. 2008.PubMed/NCBI
9	Lai T, Su C, Kuo W, Yeh Y, Kuo W, Tsai F, Tsai C, Weng Y and Huang C: β-catenin plays a key role in metastasis of human hepatocellular carcinoma. Oncology Reports. 26:415–422. 2011.PubMed/NCBI
10	Voronkov A and Krauss S: Wnt/beta-catenin signaling and small molecule inhibitors. Curr Pharm Des. 19:634–664. 2013. View Article : Google Scholar : PubMed/NCBI
11	Clevers H and Nusse R: Wnt/β-catenin signaling and disease. Cell. 149:1192–1205. 2012. View Article : Google Scholar : PubMed/NCBI
12	Schmeel LC, Schmeel FC, Kim Y, Endo T, Lu D and Schmidt-Wolf IG: Targeting the Wnt/beta-catenin pathway in multiple myeloma. Anticancer Res. 33:4719–4726. 2013.PubMed/NCBI
13	Yao H, Ashihara E and Maekawa T: Targeting the Wnt/β-catenin signaling pathway in human cancers. Expert Opin Ther Targets. 15:873–887. 2011. View Article : Google Scholar : PubMed/NCBI
14	Behrens J: Control of beta-catenin signaling in tumor development. Ann N Y Acad Sci. 910:21–35. 2000. View Article : Google Scholar : PubMed/NCBI
15	Flemr M, Malik R, Franke V, Nejepinska J, Sedlacek R, Vlahovicek K and Svoboda P: A retrotransposon-driven dicer isoform directs endogenous small interfering RNA production in mouse oocytes. Cell. 155:807–816. 2013. View Article : Google Scholar : PubMed/NCBI
16	Dogini DB, Pascoal VD, Avansini SH, Vieira AS, Pereira TC and Lopes-Cendes I: The new world of RNAs. Genet Mol Biol. 37(Suppl 1): S285–S293. 2014. View Article : Google Scholar
17	Fellmann C and Lowe SW: Stable RNA interference rules for silencing. Nat Cell Biol. 16:10–18. 2014. View Article : Google Scholar : PubMed/NCBI
18	Ismail B, Ghezali L, Gueye R, Limami Y, Pouget C, Leger DY, Martin F, Beneytout J, Duroux J, Diab-Assaf M, et al: Novel methylsulfonyl chalcones as potential antiproliferative drugs for human prostate cancer: Involvement of the intrinsic pathway of apoptosis. Int J Oncol. 43:1160–1168. 2013.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Knockdown of β‑catenin by siRNA influences proliferation, apoptosis and invasion of the colon cancer cell line SW480

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles