Open Access

Chelerythrine chloride induces apoptosis in renal cancer HEK-293 and SW-839 cell lines

  • Authors:
    • Xiao‑Meng Chen
    • Meng Zhang
    • Peng‑Li Fan
    • Yu‑Hua Qin
    • Hong‑Wei Zhao
  • View Affiliations

  • Published online on: May 5, 2016     https://doi.org/10.3892/ol.2016.4520
  • Pages: 3917-3924
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have demonstrated that the benzo[c]phenanthridine alkaloid chelerythrine chloride (CC) has inhibitory effects on various tumors. However, the anticancer activity of CC and its underlying mechanisms have not been elucidated in renal cancer cells. The present study examined the effects of CC on growth inhibition and apoptosis of renal cancer cells in vitro and in vivo. Flow cytometry and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays revealed that CC markedly suppressed the growth of HEK‑293 and human renal cancer SW‑839 cells in a time- and dose‑dependent manner. The xenograft mouse model, which was performed in nude mice, exhibited a reduced tumor growth following CC treatment. In addition, the present study revealed that CC significantly decreased the phosphorylation of extracellular signal‑regulated kinase (ERK) and Akt, which was accompanied by upregulation of p53, B-cell lymphoma 2 (Bcl‑2)‑associated X protein, cleaved caspase‑3 and cleaved poly (adenosine diphosphate-ribose) polymerase (PARP), and downregulation of Bcl‑2, caspase‑3 and PARP. Furthermore, the use of PD98059, a specific mitogen‑activated protein kinase kinase inhibitor, potentiated the proapoptotic effects of CC, which indicated that CC may induce apoptosis in renal cancer cells partly via inhibition of ERK activity. Overall, the results of the present study demonstrated that CC may be developed as a potential anticancer treatment for patients with renal cancer.
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June-2016
Volume 11 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen XM, Zhang M, Fan PL, Qin YH and Zhao HW: Chelerythrine chloride induces apoptosis in renal cancer HEK-293 and SW-839 cell lines. Oncol Lett 11: 3917-3924, 2016
APA
Chen, X., Zhang, M., Fan, P., Qin, Y., & Zhao, H. (2016). Chelerythrine chloride induces apoptosis in renal cancer HEK-293 and SW-839 cell lines. Oncology Letters, 11, 3917-3924. https://doi.org/10.3892/ol.2016.4520
MLA
Chen, X., Zhang, M., Fan, P., Qin, Y., Zhao, H."Chelerythrine chloride induces apoptosis in renal cancer HEK-293 and SW-839 cell lines". Oncology Letters 11.6 (2016): 3917-3924.
Chicago
Chen, X., Zhang, M., Fan, P., Qin, Y., Zhao, H."Chelerythrine chloride induces apoptosis in renal cancer HEK-293 and SW-839 cell lines". Oncology Letters 11, no. 6 (2016): 3917-3924. https://doi.org/10.3892/ol.2016.4520