Melanoma educates mesenchymal stromal cells towards vasculogenic mimicry

Vartanian,Amalia; Karshieva,Saida; Dombrovsky,Vladislav; Belyavsky,Alexander

doi:10.3892/ol.2016.4523

Melanoma educates mesenchymal stromal cells towards vasculogenic mimicry

Authors:
- Amalia Vartanian
- Saida Karshieva
- Vladislav Dombrovsky
- Alexander Belyavsky
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Affiliations: Department of Experimental Diagnosis and Biotherapy of Tumors, Blokhin Russian Cancer Research Center, Moscow 115478, Russia, Laboratory of Stem and Progenitor Cell Biology, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia
Published online on: May 5, 2016 https://doi.org/10.3892/ol.2016.4523
Pages: 4264-4268

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Abstract

Accumulating evidence suggests that mesenchymal stromal cells (MSCs) are recruited to the tumor, and promote tumor development and growth. The present study was performed to investigate the communication between aggressive melanoma and MSCs in vasculogenic mimicry (VM). Normal human MSCs plated on Matrigel were unable to form capillary-like structures (CLSs). By contrast, MSCs co‑cultured with aggressive melanoma cell lines, namely, Mel Cher, Mel Kor and Mel P, generated CLSs. Significantly, MSCs co‑cultured with poorly aggressive melanoma cells, namely, Mel Me, failed to form CLSs. To identify factors responsible for VM, the effects of vascular endothelial growth factor A (VEGFA), pro‑epidermal growth factor, basic fibroblast growth factor and stromal cell‑derived factor 1α on the formation of CLSs by MSCs were tested. VM was induced by the addition of VEGFA, whereas other cytokines were inefficient. To confirm the hypothesis that aggressive tumor cells can increase the vasculogenic ability of MSCs, a standard B16/F10 mouse melanoma test system was used. MSCs isolated from the adipose tissues of C57BL/6 mice with melanoma formed a vascular‑like network on Matrigel, whereas MSCs from healthy mice failed to form such structures. This study provides the first direct evidence that melanoma tumors educate MSCs to engage in VM. The education may occur distantly. These findings offer promise for novel therapeutic directions in the treatment of metastatic melanoma.

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