Open Access

A potential peptide vector that allows targeted delivery of a desired fusion protein into the human breast cancer cell line MDA‑MB‑231

  • Authors:
    • Wei Qing Liu
    • Jun Yang
    • Min Hong
    • Chang E. Gao
    • Jian Dong
  • View Affiliations

  • Published online on: May 6, 2016     https://doi.org/10.3892/ol.2016.4538
  • Pages: 3943-3952
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effective control of breast cancer has been primarily hampered by a lack of tumor specificity in treatments. One potential way to improve targeting specificity is to develop novel vectors that specifically bind to and are internalized by tumor cells. Through a phage display library, an 11‑L‑amino acid peptide, PI (sequence, CASPSGALRSC), was selected. PI was labeled with fluorescein isothiocyanate (FITC) and named PI‑FITC. Subsequently, the specific affinity of PI‑FITC to MDA‑MB‑231 human breast cancer cells and other cancer cell lines was observed by confocal microscopy. Our previous study established that PI‑FITC also shows affinity to Calu‑1 human lung carcinoma cells and major histocompatibility complex class I antigen molecules; therefore, the cytomembrane proteins of the cell lines were analyzed to determine those that were common to the two cell lines and may be associated with transmembrane transduction. To further test the delivery ability of PI to MDA‑MB‑231 cells, PI‑glutathione‑S‑transferase (GST) was constructed and the internalization of this fusion protein was visualized by immunofluorescence microscopy. The results revealed that PI exhibited specific affinity to MDA‑MB‑231 cells. Use of membrane transport inhibitors indicated that macropinocytosis and caveolin‑mediated endocytosis may be involved in the endocytosis of PI. In addition, 11 membrane proteins common to MDA‑MB‑231 and Calu‑1 may be associated with transmembrane transduction. In summary, PI was able to deliver PI‑GST into MDA‑MB‑231 cells. Thus, PI could be modified to be a potential vector, and may contribute to the development of targeted therapeutic strategies for breast cancer.
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June-2016
Volume 11 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu WQ, Yang J, Hong M, Gao CE and Dong J: A potential peptide vector that allows targeted delivery of a desired fusion protein into the human breast cancer cell line MDA‑MB‑231. Oncol Lett 11: 3943-3952, 2016
APA
Liu, W.Q., Yang, J., Hong, M., Gao, C.E., & Dong, J. (2016). A potential peptide vector that allows targeted delivery of a desired fusion protein into the human breast cancer cell line MDA‑MB‑231. Oncology Letters, 11, 3943-3952. https://doi.org/10.3892/ol.2016.4538
MLA
Liu, W. Q., Yang, J., Hong, M., Gao, C. E., Dong, J."A potential peptide vector that allows targeted delivery of a desired fusion protein into the human breast cancer cell line MDA‑MB‑231". Oncology Letters 11.6 (2016): 3943-3952.
Chicago
Liu, W. Q., Yang, J., Hong, M., Gao, C. E., Dong, J."A potential peptide vector that allows targeted delivery of a desired fusion protein into the human breast cancer cell line MDA‑MB‑231". Oncology Letters 11, no. 6 (2016): 3943-3952. https://doi.org/10.3892/ol.2016.4538