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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Construction of Ang2‑siRNA chitosan magnetic nanoparticles and the effect on Ang2 gene expression in human malignant melanoma cells

  • Authors:
    • Zhao‑Liang Liu
    • Cai‑Lian You
    • Biao Wang
    • Jian‑Hong Lin
    • Xue‑Feng Hu
    • Xiu‑Ying Shan
    • Mei‑Shui Wang
    • Hou‑Bing Zheng
    • Yan‑Ding Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Plastic Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350108, P.R. China
  • Pages: 3992-3998
    |
    Published online on: May 6, 2016
       https://doi.org/10.3892/ol.2016.4539
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Abstract

The aim of the present study was to construct angiopoietin‑2 (Ang2)‑small interfering (si)RNA chitosan magnetic nanoparticles and to observe the interference effects of the nanoparticles on the expression of the Ang2 gene in human malignant melanoma cells. Ang2‑siRNA chitosan magnetic nanoparticles were constructed and transfected into human malignant melanoma cells in vitro. Red fluorescent protein expression was observed, and the transfection efficiency was analyzed. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to assess the inhibition efficiency of Ang2 gene expression. Ang2‑siRNA chitosan magnetic nanoparticles were successfully constructed, and at a mass ratio of plasmid to magnetic chitosan nanoparticles of 1:100, the transfection efficiency into human malignant melanoma cells was the highest of the ratios assessed, reaching 61.17%. RT‑qPCR analysis showed that the magnetic chitosan nanoparticles effectively inhibited Ang2 gene expression in cells, and the inhibition efficiency reached 59.56% (P<0.05). Ang2‑siRNA chitosan magnetic nanoparticles were successfully constructed. The in vitro studies showed that the nanoparticles inhibited Ang2 gene expression in human malignant melanoma tumor cells, which laid the foundation and provided experimental evidence for additional future in vivo studies of intervention targeting malignant melanoma tumor growth in nude mice.
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1 

Parkin DM, Bray F, Ferlay J and Pisani P: Estimating the world cancer burden: Globocan 2000. Int J Cancer. 94:153–156. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Yakovleva ME, Welinder C, Sugiharaa Y, Pawlowskid K, Rezelib M, Wieslandera E, Malme J and Marko-Varga G: Workflow for large-scale analysis of melanoma tissue samples. EuPA Open Proteomics. 8:78–84. 2015. View Article : Google Scholar

3 

Essner R: Surgical treatment of malignant melanoma. Surg Clin North Am. 83:109–156. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Oliner J, Min H, Leal J, Yu D, Rao S, You E, Tang X, Kim H, Meyer S, Han SJ, et al: Suppression of angiogenesis and tumor growth by selective inhibition of angiopoietin-2. Cancer Cell. 6:507–516. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Helfrich I, Edler L, Sucker A, Thomas M, Christian S, Schadendorf D and Augustin HG: Angiopoietin-2 levels are associated with disease progression in metastatic malignant melanoma. Clin Cancer Res. 15:1384–1392. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Schlaak M, Kreuzberg N, Mauch C and Kurschat P: Personalized therapy concepts for malignant melanoma. Internist (Berl). 54:188–193. 2013.(In German). View Article : Google Scholar : PubMed/NCBI

7 

Boguslawska J and Małecki M: siRNA preparations in gene therapy of melanoma. Med Wieku Rozwoj. 17:196–201. 2013.PubMed/NCBI

8 

Dolinsek T, Markelc B, Sersa G, Coer A, Stimac M, Lavrencak J, Brozic A, Kranjc S and Cemazar M: Multiple delivery of siRNA against endoglin into murine mammary adenocarcinoma prevents angiogenesis and delays tumor growth. PLoS One. 8:e587232013. View Article : Google Scholar : PubMed/NCBI

9 

Liu ZL, Wang B, Guo GX, Shan XY, Wang MS, Zhuang FL, Cai CS, Zhang MF and Zhang YD: Construction of recombinant lentiviral vector of siRNA for Ang2 and the effect of the expression of Ang2 gene in malignant melanoma cells. J Med Mol Biol. 8:494–500. 2011.

10 

Wang B, Lin JH, Zhang WQ, Zhang MF, Liu ZL, Shan XY, Wang MS and Zhuang FL: Ang2-siRNA lentivirus interfernce on melanoma xenograft in nude mice. J Med Mol Biol. 9:79–83. 2012.

11 

Wang B, Liu Z, Zhang M, San X, Zhang Y, Zhang W and Wang M: Interfering growth of malignant melanoma with Ang2-siRNA. Mol Biol Rep. 40:1463–1471. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Kenjo E, Asai T, Yonenaga N, Ando H, Ishii T, Hatanaka K, Shimizu K, Urita Y, Dewa T, Nango M, et al: Systemic delivery of small interfering RNA by use of targeted polycation liposomes for cancer therapy. Biol Pharm Bull. 36:287–291. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Todorovic V, Sersa G and Cemazar M: Gene electrotransfer of siRNAs against CD146 inhibits migration and invasion of human malignant melanoma cells SK-MEL28. Cancer Gene Ther. 20:208–210. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Stein S, Scholz S, Schwäble J, Sadat MA, Modlich U, Schultze-Strasser S, Diaz M, Chen-Wichmann L, Müller-Kuller U, Brendel C, et al: From bench to bedside: Preclinical evaluation of a self-inactivating gammaretroviral vector for the gene therapy of X-linked chronic granulomatous disease. Hum Gene Ther Clin Dev. 24:86–98. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Bonamassa B and Liu D: Nonviral gene transfer as a tool for studying transcription regulation of xenobiotic metabolizing enzymes. Adv Drug Deliv Rev. 62:1250–1256. 2010. View Article : Google Scholar : PubMed/NCBI

16 

O'Rorke S, Keeney M and Pandit A: Non-viral polyplexes: Scaffold mediated delivery for gene therapy. Prog Polym Sci. 35:441–458. 2010. View Article : Google Scholar

17 

Sionkowska A, Wisniewski M, Skopinska J, Kennedy CJ and Wess TJ: Molecular interactions incollagen and chitosan blends. Biomaterials. 25:795–801. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Pang SW, Park HY, Jang YS, Kim WS and Kim JH: Effects of charge density and particle size of poly (styrene/(dimethylamino)ethyl methacrylate) nanoparticle for gene delivery in 293 cells. Colloids Surf B Biointerfaces. 26:213–222. 2002. View Article : Google Scholar

19 

Mansouri S, Lavigne P, Corsi K, Benderdour M, Beaumont E and Fernandes JC: Chitosan-DNA nanopartieles as non-viral vectors in gene therapy: Strategies to improve transfection efficacy. Eur J Pharm Biopharm. 57:1–8. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Sato T, Ishii T and Okahata Y: In vitro gene delivery mediated by chitosan. Effect of pH, serum, and molecular mass of chitosan on the transfection efficiency. Biomaterials. 22:2075–2080. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Kiang T, Wen J, Lim HW and Leong KW: The effect of the degree of chitosan deacetylation on the efficiency of gene transfection. Biomaterials. 25:5293–5301. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Mao HQ, Roy K, Troung-Le VL, Janes KA, Lin KY, Wang Y, August JT and Leong KW: Chitosan-DNA nanoparticles as gene carriers: Synthesis, characterization and transfection efficiency. J Control Release. 70:399–421. 2001. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu ZL, You CL, Wang B, Lin JH, Hu XF, Shan XY, Wang MS, Zheng HB and Zhang YD: Construction of Ang2‑siRNA chitosan magnetic nanoparticles and the effect on Ang2 gene expression in human malignant melanoma cells. Oncol Lett 11: 3992-3998, 2016.
APA
Liu, Z., You, C., Wang, B., Lin, J., Hu, X., Shan, X. ... Zhang, Y. (2016). Construction of Ang2‑siRNA chitosan magnetic nanoparticles and the effect on Ang2 gene expression in human malignant melanoma cells. Oncology Letters, 11, 3992-3998. https://doi.org/10.3892/ol.2016.4539
MLA
Liu, Z., You, C., Wang, B., Lin, J., Hu, X., Shan, X., Wang, M., Zheng, H., Zhang, Y."Construction of Ang2‑siRNA chitosan magnetic nanoparticles and the effect on Ang2 gene expression in human malignant melanoma cells". Oncology Letters 11.6 (2016): 3992-3998.
Chicago
Liu, Z., You, C., Wang, B., Lin, J., Hu, X., Shan, X., Wang, M., Zheng, H., Zhang, Y."Construction of Ang2‑siRNA chitosan magnetic nanoparticles and the effect on Ang2 gene expression in human malignant melanoma cells". Oncology Letters 11, no. 6 (2016): 3992-3998. https://doi.org/10.3892/ol.2016.4539
Copy and paste a formatted citation
x
Spandidos Publications style
Liu ZL, You CL, Wang B, Lin JH, Hu XF, Shan XY, Wang MS, Zheng HB and Zhang YD: Construction of Ang2‑siRNA chitosan magnetic nanoparticles and the effect on Ang2 gene expression in human malignant melanoma cells. Oncol Lett 11: 3992-3998, 2016.
APA
Liu, Z., You, C., Wang, B., Lin, J., Hu, X., Shan, X. ... Zhang, Y. (2016). Construction of Ang2‑siRNA chitosan magnetic nanoparticles and the effect on Ang2 gene expression in human malignant melanoma cells. Oncology Letters, 11, 3992-3998. https://doi.org/10.3892/ol.2016.4539
MLA
Liu, Z., You, C., Wang, B., Lin, J., Hu, X., Shan, X., Wang, M., Zheng, H., Zhang, Y."Construction of Ang2‑siRNA chitosan magnetic nanoparticles and the effect on Ang2 gene expression in human malignant melanoma cells". Oncology Letters 11.6 (2016): 3992-3998.
Chicago
Liu, Z., You, C., Wang, B., Lin, J., Hu, X., Shan, X., Wang, M., Zheng, H., Zhang, Y."Construction of Ang2‑siRNA chitosan magnetic nanoparticles and the effect on Ang2 gene expression in human malignant melanoma cells". Oncology Letters 11, no. 6 (2016): 3992-3998. https://doi.org/10.3892/ol.2016.4539
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