Aspirin inhibits growth of ovarian cancer by upregulating caspase-3 and downregulating bcl-2 Retraction in /10.3892/ol.2019.10108

Li,Lin; Mao,Xiaogang; Qin,Xiaomin; Zhou,Min; Xing,Hui; Dong,Fan; Jiang,Xiaoyuan; Zhuang,Wenhui

doi:10.3892/ol.2016.4607

July-2016 Volume 12 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

July-2016 Volume 12 Issue 1

Full Size Image

Article Open Access

Aspirin inhibits growth of ovarian cancer by upregulating caspase-3 and downregulating bcl-2

Retraction in: /10.3892/ol.2019.10108

Authors:
- Lin Li
- Xiaogang Mao
- Xiaomin Qin
- Min Zhou
- Hui Xing
- Fan Dong
- Xiaoyuan Jiang
- Wenhui Zhuang
View Affiliations / Copyright

Affiliations: Department of Gynaecology and Obstetrics, Xiangyan Central Hospital, Hubei University of Arts and Science, Xiangyang, Hubei 441021, P.R. China, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China, Dongfang Hospital, The Second Clinical Medical College of Beijing University of Chinese Medicine (BUCM), Beijing 100078, P.R. China, School of Life Sciences, Fudan Univesity, Shanghai 200433, P.R. China

Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 93-96
|
Published online on: May 18, 2016

https://doi.org/10.3892/ol.2016.4607
Expand metrics +

Abstract

The aim of the present study was to investigate the effect and mechanism of different concentrations of aspirin in inhibiting the ovarian cancer of p53N236S gene knock-in mice. In total, 28 male p53S mice, with an age range of 4-6 weeks and weight of 20-25 g were selected. The animals were transplanted with SKOV3 cells to establish subdermal human ovarian cancer. The mice were randomly divided into different groups according to the aspirin concentrations (mmol/l) used, i.e., 0, 1, 2 and 3. Subsequently, intraperitoneal injection was performed once every two days for 3 weeks. The tumor volume, lifetime, tumor cell proliferation inhibition rates, caspase-3 protein and bcl-2 protein expression of the four groups were analyzed and compared. Following aspirin treatment for 1, 2 and 3 weeks, the tumor volume of the 3 mmol/l aspirin group was significantly smaller than the other groups (p<0.05). The higher concentration of aspirin led to a smaller tumor size (p<0.05). The cell proliferation inhibition rate of the 3 mmol/l aspirin group was significantly larger than that of other groups (P<0.05). The relative expression level of caspase-3, bcl-2 protein of the 3 mmol/l aspirin group was significantly improved and reduced, respectively. In conclusion, aspirin can inhibit the growth of ovarian cancer of p53S rats due to its upregulation of the expression of caspase-3 protein and downregulation of the expression of bcl-2 protein.

View Figures

View References

1	Tarim E, Bal N, Kilicdag E, Kayaselcuk F, Bağiş T and Kuscu E: Effects of aspirin on placenta and perinatal outcomes in patients with poor obstetric history. Arch Gynecol Obstet. 274:209–214. 2006. View Article : Google Scholar : PubMed/NCBI
2	Middleton FA, Ignacio C, Camargo M, Hicks S and Mooney S: Effects of developmental ethanol exposures in wildtype and p53-null mice on transcriptional and epigenetic regulation of DNA damage repair, cell cycle, cell fate and cell death processes. Int J Dev Neurosci. 47:124–125. 2015. View Article : Google Scholar
3	Park YJ, Kim EK, Bae JY, Moon S and Kim J: Human telomerase reverse transcriptase (hTERT) promotes cancer invasion by modulating cathepsin D via early growth response (EGR)-1. Cancer Lett. 370:222–231. 2015. View Article : Google Scholar : PubMed/NCBI
4	Hansen GL, Gaudernack G, Brunsvig PF, Cvancarova M and Kyte JA: Immunological factors influencing clinical outcome in lung cancer patients after telomerase peptide vaccination. Cancer Immunol Immunother. Oct 26–2015.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI
5	Shi M, Zheng J, Liu C, Tan G, Qing Z, Yang S, Yang J, Tan Y and Yang R: SERS assay of telomerase activity at single-cell level and colon cancer tissues via quadratic signal amplification. Biosens Bioelectron. 77:673–680. 2016. View Article : Google Scholar : PubMed/NCBI
6	Moorchung N, Vasudevan B, Kumar Dinesh S and Muralidhar A: Expression of apoptosis regulating proteins p53 and bcl-2 in psoriasis. Indian J Pathol Microbiol. 58:423–426. 2015. View Article : Google Scholar : PubMed/NCBI
7	Croci DO, Cogno IS, Vittar NB, Salvatierra E, Trajtenberg F, Podhajcer OL, Osinaga E, Rabinovich GA and Rivarola VA: Silencing survivin gene expression promotes apoptosis of human breast cancer cells through a caspase-independent pathway. J Cell Biochem. 105:381–390. 2008. View Article : Google Scholar : PubMed/NCBI
8	Gordeev SA, Bykova TV, Zubova SG, Aksenov ND and Pospelova TV: Antiapoptotic gene bcl-2 prevents cellular senescence program reactivation induced by histone deacetylase inhibitor sodium butyrate in E1A and cHa-ras transformed rat fibroblasts. Tsitologiia. 57:135–143. 2015.(In Russian). PubMed/NCBI
9	Muller PA and Vousden KH: Mutant p53 in cancer: new functions and therapeutic opportunities. Cancer Cell. 25:304–317. 2014. View Article : Google Scholar : PubMed/NCBI
10	Padda RS, Gkouvatsos K, Guido M, Mui J, Vali H and Pantopoulos K: A high-fat diet modulates iron metabolism but does not promote liver fibrosis in hemochromatotic Hjv^−/− mice. Am J Physiol Gastrointest Liver Physiol. 308:G251–G261. 2015. View Article : Google Scholar : PubMed/NCBI
11	Schildkraut JM, Moorman PG, Halabi S, Calingaert B, Marks JR and Berchuck A: Analgesic drug use and risk of ovarian cancer. Epidemiology. 17:104–107. 2006. View Article : Google Scholar : PubMed/NCBI
12	Drake JG and Becker JL: Aspirin-induced inhibition of ovariantumor cell growth. Obstet Gynecol. 100:677–682. 2002. View Article : Google Scholar : PubMed/NCBI
13	Andrews P, Zhao X, Allen J, Li F and Chang M: A comparison of the effectiveness of selected non-steroidal anti-inflammatory drugs and their derivatives against cancer cells in vitro. Cancer Chemother Pharmacol. 11:203–214. 2007. View Article : Google Scholar
14	Salman MC and Ayhan A: Use of anti-thrombotic agents during chemotherapy for epithelial ovarian cancer. Med Hypotheses. 66:1179–1181. 2006. View Article : Google Scholar : PubMed/NCBI
15	McMenamin ÚC, Cardwell CR, Hughes CM and Murray LM: Low-dose aspirin and survival from lung cancer: a population-based cohort study. BMC Cancer. 15:9112015. View Article : Google Scholar : PubMed/NCBI
16	Kim B, Park SJ, Hong SP, Cheon JH, Kim WH and Kim TI: The effect of prediagnostic aspirin use on the prognosis of stage III colorectal cancer. Int J Clin Exp Med. 8:13435–13445. 2015.PubMed/NCBI