Association between the p73 gene G4C14‑to‑A4T14 single nucleotide polymorphism and risk of cervical cancer by high resolution melting and PCR with confronting two-pair primers in a Chinese population

Guo,Haiyan; Yang,Shaodi; Xu,Lijian; Li,Ding; Tang,Jianxin; Wang,Shuangshaung; Wei,Benjie; Liu,Zhengchun

doi:10.3892/ol.2016.4655

Association between the p73 gene G4C14‑to‑A4T14 single nucleotide polymorphism and risk of cervical cancer by high resolution melting and PCR with confronting two-pair primers in a Chinese population

Authors:
- Haiyan Guo
- Shaodi Yang
- Lijian Xu
- Ding Li
- Jianxin Tang
- Shuangshaung Wang
- Benjie Wei
- Zhengchun Liu
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Affiliations: Department of Obstetrics and Gynecology, Xi'an No. 4 Hospital, Xi'an, Shaanxi 710004, P.R. China, Institute of Biomedical Engineering, School of Geosciences and Info‑Physics, Central South University, Changsha, Hunan 410000, P.R. China, Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, Hunan 412000, P.R. China, Yin Feng Biological Group Co., Ltd., Jinan, Shandong 250000, P.R. China
Published online on: June 1, 2016 https://doi.org/10.3892/ol.2016.4655
Pages: 721-726

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Abstract

As a member of the p53 gene family, the p73 gene can affect an individual's susceptibility to cancer through a p53‑like manner. DNA sequence variation in the p73 gene has been reported to be associated with cancer risk. The present study aimed to identify whether the p73 gene G4C14‑to‑A4T14 single nucleotide polymorphism (SNP) is associated with risk of cervical cancer in a Chinese population. The p73 G4C14‑to‑A4T14 polymorphism was genotyped in 175 cervical cancer and 189 healthy control peripheral blood DNA samples using high resolution melting, polymerase chain reaction with confronting two‑pair primers and direct DNA sequencing. The results demonstrated that carriers of the AT/AT genotype were associated with a significantly increased risk of cervical cancer (P=0.042; χ2=4.122; odds ratio = 2.241; 95% confidence interval = 1.013‑4.956) compared with the GC/GC genotype carriers. In addition, there was a significant association between p73 genotypes and tumor size in patients with cervical cancer (P=0.014; χ2=8.607). However, no association was identified between p73 genotypes and tumor stage, histological type or lymph node metastasis in patients with cervical cancer. These results suggest that the p73 G4C14‑to‑A4T14 SNP may function as a marker of genetic susceptibility to cervical cancer in the Chinese population.

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