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Article

Involvement of p38MAPK‑ATF2 signaling pathway in alternariol induced DNA polymerase β expression

  • Authors:
    • Jimin Zhao
    • Junfen Ma
    • Jing Lu
    • Yanan Jiang
    • Yanyan Zhang
    • Xiaoyan Zhang
    • Jun Zhao
    • Hongyan Yang
    • Youtian Huang
    • Mingyao Zhao
    • Kangdong Liu
    • Ziming Dong
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China, Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Medical Oncology, Changzhi People's Hospital, Changzhi, Shanxi 046000, P.R. China
  • Pages: 675-679
    |
    Published online on: June 1, 2016
       https://doi.org/10.3892/ol.2016.4662
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Abstract

Base excision repair (BER) systems are important for maintaining the integrity of genomes in mammalian cells. Aberrant DNA bases or broken single strands can be repaired by BER. Consequently, DNA lesions, which may be caused by cancer and aging, have a close association with BER procedure. DNA polymerase β (polβ) is a critical BER enzyme that can excise 5'‑sugar phosphate prior to adding a nucleotide in the gap by its function as a DNA polymerase in the BER process. However, DNA polβ is an error‑prone DNA polymerase, and overexpressing polβ increases the cellular spontaneous mutation rate. DNA polβ overexpression has been identified in various human tumors, which implies that DNA polβ overexpression has a close association with tumorigenesis. The present study showed that alternariol (AOH), a secondary product of a fungus that is found in grains and fruits, could cause DNA damage to NIH3T3 cells in a single cell gel electrophoresis, and that 2, 10 and 20 µM AOH induced DNA polβ overexpression in a dose‑dependent manner. In the process, the level of phosphorylation of mitogen‑activated protein kinase 14 (p38) mitogen‑activated protein kinase (MAPK) and activating transcription factor 2 (ATF2) was increased. In addition, SB203580, a p38MAPK inhibitor, resulted in decreased DNA polβ expression. Small hairpin RNA‑p38MAPK had the same effect; notably, DNA polβ expression was downregulated in p38MAPK knockdown cells. These data suggest that the p38MAPK‑ATF2 signaling pathway may be involved in DNA polβ expression induced by AOH.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao J, Ma J, Lu J, Jiang Y, Zhang Y, Zhang X, Zhao J, Yang H, Huang Y, Zhao M, Zhao M, et al: Involvement of p38MAPK‑ATF2 signaling pathway in alternariol induced DNA polymerase β expression. Oncol Lett 12: 675-679, 2016.
APA
Zhao, J., Ma, J., Lu, J., Jiang, Y., Zhang, Y., Zhang, X. ... Dong, Z. (2016). Involvement of p38MAPK‑ATF2 signaling pathway in alternariol induced DNA polymerase β expression. Oncology Letters, 12, 675-679. https://doi.org/10.3892/ol.2016.4662
MLA
Zhao, J., Ma, J., Lu, J., Jiang, Y., Zhang, Y., Zhang, X., Zhao, J., Yang, H., Huang, Y., Zhao, M., Liu, K., Dong, Z."Involvement of p38MAPK‑ATF2 signaling pathway in alternariol induced DNA polymerase β expression". Oncology Letters 12.1 (2016): 675-679.
Chicago
Zhao, J., Ma, J., Lu, J., Jiang, Y., Zhang, Y., Zhang, X., Zhao, J., Yang, H., Huang, Y., Zhao, M., Liu, K., Dong, Z."Involvement of p38MAPK‑ATF2 signaling pathway in alternariol induced DNA polymerase β expression". Oncology Letters 12, no. 1 (2016): 675-679. https://doi.org/10.3892/ol.2016.4662
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao J, Ma J, Lu J, Jiang Y, Zhang Y, Zhang X, Zhao J, Yang H, Huang Y, Zhao M, Zhao M, et al: Involvement of p38MAPK‑ATF2 signaling pathway in alternariol induced DNA polymerase β expression. Oncol Lett 12: 675-679, 2016.
APA
Zhao, J., Ma, J., Lu, J., Jiang, Y., Zhang, Y., Zhang, X. ... Dong, Z. (2016). Involvement of p38MAPK‑ATF2 signaling pathway in alternariol induced DNA polymerase β expression. Oncology Letters, 12, 675-679. https://doi.org/10.3892/ol.2016.4662
MLA
Zhao, J., Ma, J., Lu, J., Jiang, Y., Zhang, Y., Zhang, X., Zhao, J., Yang, H., Huang, Y., Zhao, M., Liu, K., Dong, Z."Involvement of p38MAPK‑ATF2 signaling pathway in alternariol induced DNA polymerase β expression". Oncology Letters 12.1 (2016): 675-679.
Chicago
Zhao, J., Ma, J., Lu, J., Jiang, Y., Zhang, Y., Zhang, X., Zhao, J., Yang, H., Huang, Y., Zhao, M., Liu, K., Dong, Z."Involvement of p38MAPK‑ATF2 signaling pathway in alternariol induced DNA polymerase β expression". Oncology Letters 12, no. 1 (2016): 675-679. https://doi.org/10.3892/ol.2016.4662
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