Early detection of tumor relapse/regrowth by consecutive minimal residual disease monitoring in high-risk neuroblastoma patients

Hirase,Satoshi; Saitoh,Atsuro; Hartomo,Tri  Budi; Kozaki,Aiko; Yanai,Tomoko; Hasegawa,Daiichiro; Kawasaki,Keiichiro; Kosaka,Yoshiyuki; Matsuo,Masafumi; Yamamoto,Nobuyuki; Mori,Takeshi; Hayakawa,Akira; Iijima,Kazumoto; Nishio,Hisahide; Nishimura,Noriyuki

doi:10.3892/ol.2016.4682

August-2016 Volume 12 Issue 2

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August-2016 Volume 12 Issue 2

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Article

Early detection of tumor relapse/regrowth by consecutive minimal residual disease monitoring in high-risk neuroblastoma patients

Authors:
- Satoshi Hirase
- Atsuro Saitoh
- Tri Budi Hartomo
- Aiko Kozaki
- Tomoko Yanai
- Daiichiro Hasegawa
- Keiichiro Kawasaki
- Yoshiyuki Kosaka
- Masafumi Matsuo
- Nobuyuki Yamamoto
- Takeshi Mori
- Akira Hayakawa
- Kazumoto Iijima
- Hisahide Nishio
- Noriyuki Nishimura
View Affiliations / Copyright

Affiliations: Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe 650‑0017, Japan, Department of Hematology and Oncology, Kobe Children's Hospital, Kobe 654‑0081, Japan, Department of Epidemiology, Kobe University Graduate School of Medicine, Kobe 650‑0017, Japan, Department of Medical Rehabilitation, Kobe Gakuin University, Kobe 651‑2180, Japan
Pages: 1119-1123
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Published online on: June 7, 2016

https://doi.org/10.3892/ol.2016.4682
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Abstract

Neuroblastoma is an aggressive pediatric tumor accounting for ~15% of cancer-associated mortalities in children. Despite the current intensive therapy, >50% of high-risk patients experience tumor relapse or regrowth caused by the activation of minimal residual disease (MRD). Although several MRD detection protocols using various reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) markers have been reported to evaluate the therapeutic response and disease status of neuroblastoma patients, their clinical significance remains elusive. The present study reports two high‑risk neuroblastoma patients, whose MRD was consecutively monitored using 11 RT-qPCR markers (CHRNA3, CRMP1, DBH, DCX, DDC, GABRB3, GAP43, ISL1, KIF1A, PHOX2B and TH) during their course of treatment. The two patients initially responded to the induction therapy and reached MRD‑negative status. The patients' MRD subsequently became positive with no elevation of their urinary homovanillic acid, urinary vanillylmandelic acid and serum neuron‑specific enolase levels at 13 or 19 weeks prior to the clinical diagnosis of tumor relapse or regrowth. The present cases highlight the possibility of consecutive MRD monitoring using 11 markers to enable an early detection of tumor relapse or regrowth in high‑risk neuroblastoma patients.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Early detection of tumor relapse/regrowth by consecutive minimal residual disease monitoring in high-risk neuroblastoma patients

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