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Article

CD147 modulates androgen receptor activity through the Akt/Gsk-3β/β-catenin/AR pathway in prostate cancer cells

  • Authors:
    • Fang Fang
    • Yingxin Qin
    • Feng Hao
    • Qiang Li
    • Wei Zhang
    • Chen Zhao
    • Shuang Chen
    • Liangzhong Zhao
    • Liguo Wang
    • Jianhui Cai
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, Jilin Medical University, Jilin 132013, P.R. China, Department of Anesthesiology, Affiliated Hospital of Jilin Medical University, Jilin 132011, P.R. China, Department of Biochemistry, Jilin Medical University, Jilin 132013, P.R. China, Department of Urology Surgery, Affiliated Hospital of Jilin Medical University, Jilin 132011, P.R. China
  • Pages: 1124-1128
    |
    Published online on: June 7, 2016
       https://doi.org/10.3892/ol.2016.4684
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Abstract

The androgen signaling pathway serves an important role in the development of prostate cancer. β-Catenin is an androgen receptor (AR) cofactor and augments AR signaling. Glycogen synthase kinase-3β (GSK-3β), a target of phosphorylated serine/threonine protein kinase B (p-Akt), regulates β-catenin stability. In addition, β-catenin, a coregulator of AR, physically interacts with AR and enhances AR‑mediated target gene transcription. The multifunctional glycoprotein cluster of differentiation (CD) 147 is highly expressed on the cell surface of the majority of cancer cells, and it promotes tumor invasion, metastasis and growth. In the present study, the molecular effects of CD147 on the Akt/GSK‑3β/β‑catenin/AR signaling network were investigated in LNCaP cells. Using short hairpin‑mediated RNA knockdown of CD147 in LNCaP cells, it was demonstrated that downregulation of CD147 resulted in inhibitory phosphorylation of GSK‑3β, and then promoted degeneration of β‑catenin and reduced nuclear accumulation of β-catenin. In addition, immunoprecipitation studies demonstrated that CD147 downregulation decreased the formation of a complex between β‑catenin and AR. It was shown that CD147 knockdown suppressed the expression of the AR target gene prostate-specific antigen and the growth of AR‑positive LNCaP cells. Furthermore, inhibition of PI3K/Akt with LY294002 augmented CD147-mediated function. The present study indicates that the PI3K/Akt pathway may facilitate CD147-mediated activation of the AR pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Fang F, Qin Y, Hao F, Li Q, Zhang W, Zhao C, Chen S, Zhao L, Wang L, Cai J, Cai J, et al: CD147 modulates androgen receptor activity through the Akt/Gsk-3β/β-catenin/AR pathway in prostate cancer cells. Oncol Lett 12: 1124-1128, 2016.
APA
Fang, F., Qin, Y., Hao, F., Li, Q., Zhang, W., Zhao, C. ... Cai, J. (2016). CD147 modulates androgen receptor activity through the Akt/Gsk-3β/β-catenin/AR pathway in prostate cancer cells. Oncology Letters, 12, 1124-1128. https://doi.org/10.3892/ol.2016.4684
MLA
Fang, F., Qin, Y., Hao, F., Li, Q., Zhang, W., Zhao, C., Chen, S., Zhao, L., Wang, L., Cai, J."CD147 modulates androgen receptor activity through the Akt/Gsk-3β/β-catenin/AR pathway in prostate cancer cells". Oncology Letters 12.2 (2016): 1124-1128.
Chicago
Fang, F., Qin, Y., Hao, F., Li, Q., Zhang, W., Zhao, C., Chen, S., Zhao, L., Wang, L., Cai, J."CD147 modulates androgen receptor activity through the Akt/Gsk-3β/β-catenin/AR pathway in prostate cancer cells". Oncology Letters 12, no. 2 (2016): 1124-1128. https://doi.org/10.3892/ol.2016.4684
Copy and paste a formatted citation
x
Spandidos Publications style
Fang F, Qin Y, Hao F, Li Q, Zhang W, Zhao C, Chen S, Zhao L, Wang L, Cai J, Cai J, et al: CD147 modulates androgen receptor activity through the Akt/Gsk-3β/β-catenin/AR pathway in prostate cancer cells. Oncol Lett 12: 1124-1128, 2016.
APA
Fang, F., Qin, Y., Hao, F., Li, Q., Zhang, W., Zhao, C. ... Cai, J. (2016). CD147 modulates androgen receptor activity through the Akt/Gsk-3β/β-catenin/AR pathway in prostate cancer cells. Oncology Letters, 12, 1124-1128. https://doi.org/10.3892/ol.2016.4684
MLA
Fang, F., Qin, Y., Hao, F., Li, Q., Zhang, W., Zhao, C., Chen, S., Zhao, L., Wang, L., Cai, J."CD147 modulates androgen receptor activity through the Akt/Gsk-3β/β-catenin/AR pathway in prostate cancer cells". Oncology Letters 12.2 (2016): 1124-1128.
Chicago
Fang, F., Qin, Y., Hao, F., Li, Q., Zhang, W., Zhao, C., Chen, S., Zhao, L., Wang, L., Cai, J."CD147 modulates androgen receptor activity through the Akt/Gsk-3β/β-catenin/AR pathway in prostate cancer cells". Oncology Letters 12, no. 2 (2016): 1124-1128. https://doi.org/10.3892/ol.2016.4684
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