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Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro

  • Authors:
    • Kai Wang
    • Xiao‑Kang Zhou
    • Min Wu
    • Fei‑Wu Kang
    • Zuo‑Lin Wang
    • Yan Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 863-870
    |
    Published online on: June 15, 2016
       https://doi.org/10.3892/ol.2016.4719
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Abstract

The objective of the present study was to determine the ‘stemness’ characteristics of CD133+ cells (harvested from the squamous cell tongue carcinoma Tca-8113 cell line) in vitro and to observe the tumourigenicity of the CD133+ cells in the bodies of NOD/SCID mice. Single cells from the Tca‑8113 cell line were observed for multiplication capacity in vitro. The suspending and pelletizing phenomena of Tca‑8113 cells in vitro were also observed, and the expression of CD133 in squamous cell carcinoma of the tongue was measured. The CD133+ cells from the Tca‑8113 cell line were purified, and their multiplication capacity and differentiation potency were observed. The NOD/SCID mouse model was established, and the tumourigenicity of the CD133+ cells was determined. The Tca‑8113 cells were observed to emerge in the form of suspending tumour spheres in squamous cell carcinoma of the tongue. Monoplasts with sustainable multiplication capacity accounted for ~5.32% of the spheres, and 0.95% of the CD133+ cells were expressed in squamous cell carcinoma of the tongue, with stronger multiplication capacity and differentiation potency in vitro. Stronger tumourigenicity was also observed in the bodies of the NOD/SCID mice. CD133– cells exhibited a multiplication capacity to a certain extent. Overall, the CD133+ cells in squamous cell carcinoma of the tongue are characterised by relatively strong tumourigenicity capacity in vivo and in vitro. To a certain extent, these CD133+ cells demonstrate the characteristics of ‘stemness’.
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Copy and paste a formatted citation
Spandidos Publications style
Wang K, Zhou XK, Wu M, Kang FW, Wang ZL and Zhu Y: Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro. Oncol Lett 12: 863-870, 2016.
APA
Wang, K., Zhou, X., Wu, M., Kang, F., Wang, Z., & Zhu, Y. (2016). Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro. Oncology Letters, 12, 863-870. https://doi.org/10.3892/ol.2016.4719
MLA
Wang, K., Zhou, X., Wu, M., Kang, F., Wang, Z., Zhu, Y."Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro". Oncology Letters 12.2 (2016): 863-870.
Chicago
Wang, K., Zhou, X., Wu, M., Kang, F., Wang, Z., Zhu, Y."Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro". Oncology Letters 12, no. 2 (2016): 863-870. https://doi.org/10.3892/ol.2016.4719
Copy and paste a formatted citation
x
Spandidos Publications style
Wang K, Zhou XK, Wu M, Kang FW, Wang ZL and Zhu Y: Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro. Oncol Lett 12: 863-870, 2016.
APA
Wang, K., Zhou, X., Wu, M., Kang, F., Wang, Z., & Zhu, Y. (2016). Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro. Oncology Letters, 12, 863-870. https://doi.org/10.3892/ol.2016.4719
MLA
Wang, K., Zhou, X., Wu, M., Kang, F., Wang, Z., Zhu, Y."Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro". Oncology Letters 12.2 (2016): 863-870.
Chicago
Wang, K., Zhou, X., Wu, M., Kang, F., Wang, Z., Zhu, Y."Role of CD133+ cells in tongue squamous carcinomas: Characteristics of ‘stemness’ in vivo and in vitro". Oncology Letters 12, no. 2 (2016): 863-870. https://doi.org/10.3892/ol.2016.4719
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