Expression levels of JNK associated with polymorphic lactotransferrin haplotypes in human nasopharyngeal carcinoma

Luo,Gengqiu; Zhou,Yanhong; Yi,Wei; Yi,Hong

doi:10.3892/ol.2016.4723

Expression levels of JNK associated with polymorphic lactotransferrin haplotypes in human nasopharyngeal carcinoma

Authors:
- Gengqiu Luo
- Yanhong Zhou
- Wei Yi
- Hong Yi
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Affiliations: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Molecular Genetics Laboratory, Cancer Research Institute, Central South University, Changsha, Hunan 410078, P.R. China, Research Center of Carcinogenesis and Targeted Therapy, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
Published online on: June 15, 2016 https://doi.org/10.3892/ol.2016.4723
Pages: 1085-1094

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Abstract

Lactotransferrin (LTF), a member of the transferrin family, serves a role in the innate immune response and is involved in anti‑inflammatory, anti‑microbial and anti‑tumor activity. Alterations in the LTF gene are associated with an increased incidence of cancer. The LTF gene is polymorphic, and several common alleles may be observed in the general population. Our previous study identified a lower rate of occurrence of the ʻA‑G‑G‑Tʼ haplotype (constructed with rs1126477, rs1126478, rs2073495 and rs9110) in nasopharyngeal carcinoma (NPC) patients compared with controls. In the present study, in order to elucidate a possible mechanism of LTF‑mediated anti‑tumor activity in NPC, the protein profiles of NPC and non‑tumorous nasopharyngeal epithelium tissues with/without the ʻA‑G‑G‑Tʼ haplotype were constructed using LTQ Orbitrap technology. The results revealed that c‑Jun N‑terminal kinase 2 (JNK2) was highly expressed in NPC tissues and non‑tumor nasopharyngeal epithelium tissues without the ʻA‑G‑G‑Tʼ haplotype. These results were confirmed by western blot analysis. Furthermore, microRNA (miRNA) microarray analysis was conducted to investigate the differential miRNA profiles of NPC and non‑tumor nasopharyngeal epithelium tissues with/without the ʻA‑G‑G‑Tʼ haplotype. It was observed that hsa‑miR‑1256 and hsa‑miR‑659, which are potentially targeted to the JNK2 gene, were downregulated in NPC tissues without the ʻA‑G‑G‑Tʼ haplotype. Hsa‑miR‑298, another miRNA potentially targeted to the JNK2 gene, was downregulated in non‑tumor nasopharyngeal epithelium tissues without the ʻA‑G‑G‑Tʼ haplotype. In summary, these results suggested that the expression levels of JNK2 may be associated with polymorphic LTF haplotypes in human NPC.

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