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Article

Plumbagin exhibits an anti‑proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β‑catenin signalling

  • Authors:
    • Yuan‑Liang Xue
    • Xiang‑Qi Meng
    • Long‑Jun Ma
    • Zhen Yuan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics of Clinical College, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250014, P.R. China, Department of Orthopedics, Suzhou Hospital of Traditional Chinese Medicine, Suzhou, Jiangsu 215000, P.R. China, Department of Orthopedics, People's Hospital of Yangxin, Binzhou, Shandong 251800, P.R. China, Department of Orthopedics, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, P.R. China
  • Pages: 1095-1100
    |
    Published online on: June 15, 2016
       https://doi.org/10.3892/ol.2016.4725
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Abstract

Plumbagin, a naphthoquinone constituent of Plumbago zeylanica L. (Plumbaginaceae) is widely used in traditional Chinese medicine as an antifungal, antibacterial and anti‑inflammatory agent. Plumbagin is known to exhibit proapoptotic, antiangiogenic and antimetastatic effects in cancer cells. The transcriptional co‑factor four and a half LIM domains 2 (FHL2) is a multifunctional adaptor protein that is involved in the regulation of gene expression, signal transduction and cell proliferation and differentiation, and also acts as a tumor suppressor or oncoprotein depending on the tissue microenvironment. The present study investigated the effect of plumbagin on FHL2 expression, Wnt/β‑catenin signalling and its anti‑proliferative activity in various human osteosarcoma cell lines, including SaOS2, MG63, HOS and U2OS. The cells were exposed to plumbagin and the expression of FHL2 was evaluated using western blot analysis. Furthermore, the anti‑proliferative effect of plumbagin was evaluated using a 3‑(4,5 dimethyl‑thiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. In addition, since FHL2 is involved in Wnt/β‑catenin signaling, the effect of plumbagin on β‑catenin and its primary target genes, including v‑myc avian myelocytomatosis viral oncogene homolog (c‑Myc) and WNT1 inducible signaling pathway protein‑1 (WISP‑1), was evaluated using western blot analysis. It was observed that plumbagin suppressed the expression of FHL2 and exhibited significant anti‑proliferative activity in osteosarcoma cells. It also attenuated Wnt/β‑catenin signalling by downregulating β‑catenin and its target genes, including c‑Myc and WISP‑1. In conclusion, plumbagin demonstrated anti‑proliferative activity in osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β‑catenin signalling.
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Copy and paste a formatted citation
Spandidos Publications style
Xue YL, Meng XQ, Ma LJ and Yuan Z: Plumbagin exhibits an anti‑proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β‑catenin signalling. Oncol Lett 12: 1095-1100, 2016.
APA
Xue, Y., Meng, X., Ma, L., & Yuan, Z. (2016). Plumbagin exhibits an anti‑proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β‑catenin signalling. Oncology Letters, 12, 1095-1100. https://doi.org/10.3892/ol.2016.4725
MLA
Xue, Y., Meng, X., Ma, L., Yuan, Z."Plumbagin exhibits an anti‑proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β‑catenin signalling". Oncology Letters 12.2 (2016): 1095-1100.
Chicago
Xue, Y., Meng, X., Ma, L., Yuan, Z."Plumbagin exhibits an anti‑proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β‑catenin signalling". Oncology Letters 12, no. 2 (2016): 1095-1100. https://doi.org/10.3892/ol.2016.4725
Copy and paste a formatted citation
x
Spandidos Publications style
Xue YL, Meng XQ, Ma LJ and Yuan Z: Plumbagin exhibits an anti‑proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β‑catenin signalling. Oncol Lett 12: 1095-1100, 2016.
APA
Xue, Y., Meng, X., Ma, L., & Yuan, Z. (2016). Plumbagin exhibits an anti‑proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β‑catenin signalling. Oncology Letters, 12, 1095-1100. https://doi.org/10.3892/ol.2016.4725
MLA
Xue, Y., Meng, X., Ma, L., Yuan, Z."Plumbagin exhibits an anti‑proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β‑catenin signalling". Oncology Letters 12.2 (2016): 1095-1100.
Chicago
Xue, Y., Meng, X., Ma, L., Yuan, Z."Plumbagin exhibits an anti‑proliferative effect in human osteosarcoma cells by downregulating FHL2 and interfering with Wnt/β‑catenin signalling". Oncology Letters 12, no. 2 (2016): 1095-1100. https://doi.org/10.3892/ol.2016.4725
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