Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells

  • Authors:
    • Dawei Li
    • Liang Zhou
    • Jiameng Huang
    • Xiyan Xiao
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  • Published online on: June 17, 2016     https://doi.org/10.3892/ol.2016.4749
  • Pages: 1569-1574
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Abstract

In a previous study, it was demonstrated that hypoxia upregulated the multidrug resistance (MDR) of laryngeal cancer cells to chemotherapeutic drugs, with multidrug resistance 1 (MDR1)/P-glycoprotein (P‑gp) expression also being upregulated. The present study aimed to investigate the role and mechanism of MDR1/P‑gp on hypoxia-induced MDR in human laryngeal carcinoma cells. The sensitivity of laryngeal cancer cells to multiple drugs and cisplatin‑induced apoptosis was determined by CCK‑8 assay and Annexin‑V/propidium iodide staining analysis, respectively. The accumulation of rhodamine 123 (Rh123) in the cells served as an estimate of drug accumulation and was evaluated by flow cytometry (FCM). MDR1/P-gp expression was inhibited using interference RNA, and the expression of the MDR1 gene was analyzed using reverse transcription-quantitative polymerase chain reaction and western blotting. As a result, the sensitivity to multiple chemotherapeutic agents and the apoptosis rate of the hypoxic laryngeal carcinoma cells increased following a decrease in MDR1/P‑gp expression (P<0.05). Additionally, FCM analysis of fluorescence intensity indicated that the downregulated expression of MDR1/P‑gp markedly increased intracellular Rh123 accumulation (P<0.05). Such results suggest that MDR1/P‑gp serves an important role in regulating hypoxia-induced MDR in human laryngeal carcinoma cells through a decrease in intracellular drug accumulation.
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August-2016
Volume 12 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li D, Zhou L, Huang J and Xiao X: Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells. Oncol Lett 12: 1569-1574, 2016.
APA
Li, D., Zhou, L., Huang, J., & Xiao, X. (2016). Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells. Oncology Letters, 12, 1569-1574. https://doi.org/10.3892/ol.2016.4749
MLA
Li, D., Zhou, L., Huang, J., Xiao, X."Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells". Oncology Letters 12.2 (2016): 1569-1574.
Chicago
Li, D., Zhou, L., Huang, J., Xiao, X."Effect of multidrug resistance 1/P-glycoprotein on the hypoxia-induced multidrug resistance of human laryngeal cancer cells". Oncology Letters 12, no. 2 (2016): 1569-1574. https://doi.org/10.3892/ol.2016.4749