Open Access

Identification of key target genes and pathways in laryngeal carcinoma

  • Authors:
    • Feng Liu
    • Jintao Du
    • Jun Liu
    • Bei Wen
  • View Affiliations

  • Published online on: June 17, 2016     https://doi.org/10.3892/ol.2016.4750
  • Pages: 1279-1286
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The purpose of the present study was to screen the key genes associated with laryngeal carcinoma and to investigate the molecular mechanism of laryngeal carcinoma progression. The gene expression profile of GSE10935 [Gene Expression Omnibus (GEO) accession number], including 12 specimens from laryngeal papillomas and 12 specimens from normal laryngeal epithelia controls, was downloaded from the GEO database. Differentially expressed genes (DEGs) were screened in laryngeal papillomas compared with normal controls using Limma package in R language, followed by Gene Ontology (GO) enrichment analysis and pathway enrichment analysis. Furthermore, the protein‑protein interaction (PPI) network of DEGs was constructed using Cytoscape software and modules were analyzed using MCODE plugin from the PPI network. Furthermore, significant biological pathway regions (sub‑pathway) were identified by using iSubpathwayMiner analysis. A total of 67 DEGs were identified, including 27 up‑regulated genes and 40 down‑regulated genes and they were involved in different GO terms and pathways. PPI network analysis revealed that Ras association (RalGDS/AF‑6) domain family member 1 (RASSF1) was a hub protein. The sub‑pathway analysis identified 9 significantly enriched sub‑pathways, including glycolysis/gluconeogenesis and nitrogen metabolism. Genes such as phosphoglycerate kinase 1 (PGK1), carbonic anhydrase II (CA2), and carbonic anhydrase XII (CA12) whose node degrees were >10 were identified in the disease risk sub‑pathway. Genes in the sub-pathway, such as RASSF1, PGK1, CA2 and CA12 were presumed to serve critical roles in laryngeal carcinoma. The present study identified DEGs and their sub-pathways in the disease, which may serve as potential targets for treatment of laryngeal carcinoma.
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August-2016
Volume 12 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu F, Du J, Liu J and Wen B: Identification of key target genes and pathways in laryngeal carcinoma. Oncol Lett 12: 1279-1286, 2016
APA
Liu, F., Du, J., Liu, J., & Wen, B. (2016). Identification of key target genes and pathways in laryngeal carcinoma. Oncology Letters, 12, 1279-1286. https://doi.org/10.3892/ol.2016.4750
MLA
Liu, F., Du, J., Liu, J., Wen, B."Identification of key target genes and pathways in laryngeal carcinoma". Oncology Letters 12.2 (2016): 1279-1286.
Chicago
Liu, F., Du, J., Liu, J., Wen, B."Identification of key target genes and pathways in laryngeal carcinoma". Oncology Letters 12, no. 2 (2016): 1279-1286. https://doi.org/10.3892/ol.2016.4750