Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
August-2016 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2016 Volume 12 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia)

  • Authors:
    • María Sánchez‑Ares
    • José M. Cameselle‑Teijeiro
    • Sergio Vázquez‑Estévez
    • Martín Lázaro‑Quintela
    • Ángel Vázquez‑Boquete
    • Francisco J. Afonso‑Afonso
    • Joaquín Casal‑Rubio
    • Ana L. González‑Piñeiro
    • Yolanda Rico‑Rodríguez
    • José L. Fírvida‑Pérez
    • Juan Ruíz‑Bañobre
    • Elena Couso
    • Lucía Santomé
    • Raquel Pérez‑Becerra
    • Rosario García‑Campelo
    • Margarita Amenedo
    • Cristina Azpitarte‑Raposeiras
    • José Antúnez
    • Ihab Abdulkader
  • View Affiliations / Copyright

    Affiliations: Department of Anatomic Pathology, Clinical University Hospital, Galician Healthcare Service (SERGAS), 15706 Santiago de Compostela, Spain, Department of Medical Oncology, Lucus Augusti University Hospital, SERGAS, 27003 Lugo, Spain, Department of Medical Oncology, University Hospital Complex, SERGAS, 36312 Vigo, Spain, Department of Medical Oncology, University Hospital Complex Arquitecto Marcide‑Novoa Santos, SERGAS, 15405 Ferrol, Spain, Department of Anatomic Pathology, University Hospital Complex, SERGAS, 36312 Vigo, Spain, Department of Medical Oncology, Clinical University Hospital, SERGAS, 32005 Ourense, Spain, Department of Medical Oncology, Clinical University Hospital, SERGAS, 15706 Santiago de Compostela, Spain, Department of Medical Oncology, Povisa Hospital, 36211 Vigo, Spain, Department of Medical Oncology, Clinical University Hospital, SERGAS, 15006 A Coruña, Spain, Department of Medical Oncology, Centro Oncológico de Galicia, 15009 A Coruña, Spain, Department of Medical Oncology, Clinical University Hospital, SERGAS, 36164 Pontevedra, Spain
    Copyright: © Sánchez‑Ares et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1403-1407
    |
    Published online on: June 28, 2016
       https://doi.org/10.3892/ol.2016.4788
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Identification of anaplastic lymphoma receptor tyrosine kinase (ALK) gene rearrangements is a standard diagnostic test in patients with advanced non-small cell lung cancer (NSCLC). The current study describes the experience of ALK rearrangement detection of a referral center in the public health care system of Galicia in North‑Western Spain. The fluorescence in situ hybridization (FISH) patterns of the ALK gene and the clinical and pathological features of these patients are reported. This study is also of interest for comparative purposes due to the relative geographical isolation of the area, which could have contributed to particular genetic features. A total of 2,045 tissue samples from NSCLC patients were collected between October 2010 and July 2015 and tested for ALK rearrangements by FISH. Examination of 1,686 paraffin‑embedded tissue specimens and 395 cytological samples (306 cell block preparations and 53 cytological smears) was conducted, and any associations between the FISH results and clinicopathological features were assessed. The rate of successful evaluation was marginally higher in tissue samples than in cytological samples (92.9% vs. 84.1%); this difference was not significant. ALK rearrangements were identified in 82 patients(4%): 65 (79.3%) in tissue specimens, 15 (18.3%) in cell block samples and 2 (2.4%) in cytological smears. This genetic translocation appeared to be associated with a non‑smoking history, younger age, female gender, stage IV and adenocarcinoma histological type. The findings demonstrate that ALK evaluation by FISH is feasible in tissue and cytological samples. The clinical and pathological features of the ALK‑positive series of patients are similar to those previously reported in the literature.
View Figures

Figure 1

Figure 2

View References

1 

Travis WD, Brambilla E, Burke AP, Marx A and Nicholson AG: Tumours of the lung. Who Classification Of Tumours Of Lung, Pleura, Thymus And Heart (4th). IARC Press. (Lyon, France). 9–151. 2015.

2 

Kwak EL, Bang YJ, Camidge DR, Shaw AT, Solomon B, Maki RG, Ou SH, Dezube BJ, Jänne PA, Costa DB, et al: Anaplastic lymphoma kinase inhibition in non-small-cell lung cancer. N Engl J Med. 363:1693–1703. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Patel JN, Ersek JL and Kim ES: Lung cancer biomarkers, targeted therapies and clinical assays. Transl Lung Cancer Res. 4:503–514. 2015.PubMed/NCBI

4 

Lindeman NI, Cagle PT, Beasley MB, Chitale DA, Dacic S, Giaccone G, Jenkins RB, Kwiatkowski DJ, Saldivar JS, Squire J, et al: Molecular testing guideline for selection of lung cancer patients for EGFR and ALK tyrosine kinase inhibitors: Guideline from the College of American pathologists, international association for the study of lung cancer, and association for molecular pathology. J Thorac Oncol. 8:823–859. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Soda M, Choi YL, Enomoto M, Takada S, Yamashita Y, Ishikawa S, Fujiwara S, Watanabe H, Kurashina K, Hatanaka H, et al: Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer. Nature. 448:561–566. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Vidal J, Clavé S, de Muga S, González I, Pijuan L, Gimeno J, Remón J, Reguart N, Viñolas N, Gironés R, et al: Assessment of ALK status by FISH on 1,000 Spanish non-small cell lung cancer patients. J Thorac Oncol. 9:1816–1820. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Alì G, Proietti A, Pelliccioni S, Niccoli C, Lupi C, Sensi E, Giannini R, Borrelli N, Menghi M, Chella A, et al: ALK rearrangement in a large series of consecutive non-small cell lung cancers: Comparison between a new immunohistochemical approach and fluorescence in situ hybridization for the screening of patients eligible for crizotinib treatment. Arch Pathol Lab Med. 138:1449–1458. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Cabillic F, Gros A, Dugay F, Begueret H, Mesturoux L, Chiforeanu DC, Dufrenot L, Jauffret V, Dachary D, Corre R, et al: Parallel FISH and immunohistochemical studies of ALK status in 3,244 non-small-cell lung cancers reveal major discordances. J Thorac Oncol. 9:295–306. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Zhao F, Xu M, Lei H, Zhou Z, Wang L, Li P, Zhao J and Hu P: Clinicopathological characteristics of patients with non-small-cell lung cancer who harbor EML4-ALK fusion gene: A meta-analysis. PLoS One. 10:e01173332015. View Article : Google Scholar : PubMed/NCBI

10 

Ou SH, Klempner SJ, Greenbowe JR, Azada M, Schrock AB, Ali SM, Ross JS, Stephens PJ and Miller VA: Identification of a novel HIP1-ALK fusion variant in non-small-cell lung cancer (NSCLC) and discovery of ALK I1171 (I1171N/S) mutations in two ALK-rearranged NSCLC patients with resistance to Alectinib. J Thorac Oncol. 9:1821–1825. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Iyevleva AG, Raskin GA, Tiurin VI, Sokolenko AP, Mitiushkina NV, Aleksakhina SN, Garifullina AR, Strelkova TN, Merkulov VO, Ivantsov AO, et al: Novel ALK fusion partners in lung cancer. Cancer Lett. 362:116–121. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Conde E, Suárez-Gauthier A, Benito A, Garrido P, García-Campelo R, Biscuola M, Paz-Ares L, Hardisson D, de Castro J, Camacho MC, et al: Accurate identification of ALK positive lung carcinoma patients: Novel FDA-cleared automated fluorescence in situ hybridization scanning system and ultrasensitive immunohistochemistry. PLoS One. 9:e1072002014. View Article : Google Scholar : PubMed/NCBI

13 

Wang Q, Yang X, He Y, Ma Q, Lin L, Fu P and Xiao H: Droplet digital PCR for absolute quantification of EML4-ALK gene rearrangement in lung adenocarcinoma. J Mol Diagn. 17:515–520. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Mancikova V, Cruz R, Inglada-Pérez L, Fernández-Rozadilla C, Landa I, Cameselle-Teijeiro J, Celeiro C, Pastor S, Velázquez A, Marcos R, et al: Thyroid cancer GWAS identifies 10q26.12 and 6q14.1 as novel susceptibility loci and reveals genetic heterogeneity among populations. Int J Cancer. 137:1870–1878. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Bozzetti C, Nizzoli R, Tiseo M, Squadrilli A, Lagrasta C, Buti S, Gasparro D, Zanoni D, Majori M, De Filippo M, et al: ALK and ROS1 rearrangements tested by fluorescence in situ hybridization in cytological smears from advanced non-small cell lung cancer patients. Diagn Cytopathol. 43:941–946. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Proietti A, Alì G, Pelliccioni S, Lupi C, Sensi E, Boldrini L, Servadio A, Chella A, Ribechini A, Cappuzzo F, et al: Anaplastic lymphoma kinase gene rearrangements in cytological samples of non-small cell lung cancer: Comparison with histological assessment. Cancer Cytopathol. 122:445–1453. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sánchez‑Ares M, Cameselle‑Teijeiro JM, Vázquez‑Estévez S, Lázaro‑Quintela M, Vázquez‑Boquete Á, Afonso‑Afonso FJ, Casal‑Rubio J, González‑Piñeiro AL, Rico‑Rodríguez Y, Fírvida‑Pérez JL, Fírvida‑Pérez JL, et al: Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia). Oncol Lett 12: 1403-1407, 2016.
APA
Sánchez‑Ares, M., Cameselle‑Teijeiro, J.M., Vázquez‑Estévez, S., Lázaro‑Quintela, M., Vázquez‑Boquete, Á., Afonso‑Afonso, F.J. ... Abdulkader, I. (2016). Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia). Oncology Letters, 12, 1403-1407. https://doi.org/10.3892/ol.2016.4788
MLA
Sánchez‑Ares, M., Cameselle‑Teijeiro, J. M., Vázquez‑Estévez, S., Lázaro‑Quintela, M., Vázquez‑Boquete, Á., Afonso‑Afonso, F. J., Casal‑Rubio, J., González‑Piñeiro, A. L., Rico‑Rodríguez, Y., Fírvida‑Pérez, J. L., Ruíz‑Bañobre, J., Couso, E., Santomé, L., Pérez‑Becerra, R., García‑Campelo, R., Amenedo, M., Azpitarte‑Raposeiras, C., Antúnez, J., Abdulkader, I."Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia)". Oncology Letters 12.2 (2016): 1403-1407.
Chicago
Sánchez‑Ares, M., Cameselle‑Teijeiro, J. M., Vázquez‑Estévez, S., Lázaro‑Quintela, M., Vázquez‑Boquete, Á., Afonso‑Afonso, F. J., Casal‑Rubio, J., González‑Piñeiro, A. L., Rico‑Rodríguez, Y., Fírvida‑Pérez, J. L., Ruíz‑Bañobre, J., Couso, E., Santomé, L., Pérez‑Becerra, R., García‑Campelo, R., Amenedo, M., Azpitarte‑Raposeiras, C., Antúnez, J., Abdulkader, I."Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia)". Oncology Letters 12, no. 2 (2016): 1403-1407. https://doi.org/10.3892/ol.2016.4788
Copy and paste a formatted citation
x
Spandidos Publications style
Sánchez‑Ares M, Cameselle‑Teijeiro JM, Vázquez‑Estévez S, Lázaro‑Quintela M, Vázquez‑Boquete Á, Afonso‑Afonso FJ, Casal‑Rubio J, González‑Piñeiro AL, Rico‑Rodríguez Y, Fírvida‑Pérez JL, Fírvida‑Pérez JL, et al: Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia). Oncol Lett 12: 1403-1407, 2016.
APA
Sánchez‑Ares, M., Cameselle‑Teijeiro, J.M., Vázquez‑Estévez, S., Lázaro‑Quintela, M., Vázquez‑Boquete, Á., Afonso‑Afonso, F.J. ... Abdulkader, I. (2016). Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia). Oncology Letters, 12, 1403-1407. https://doi.org/10.3892/ol.2016.4788
MLA
Sánchez‑Ares, M., Cameselle‑Teijeiro, J. M., Vázquez‑Estévez, S., Lázaro‑Quintela, M., Vázquez‑Boquete, Á., Afonso‑Afonso, F. J., Casal‑Rubio, J., González‑Piñeiro, A. L., Rico‑Rodríguez, Y., Fírvida‑Pérez, J. L., Ruíz‑Bañobre, J., Couso, E., Santomé, L., Pérez‑Becerra, R., García‑Campelo, R., Amenedo, M., Azpitarte‑Raposeiras, C., Antúnez, J., Abdulkader, I."Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia)". Oncology Letters 12.2 (2016): 1403-1407.
Chicago
Sánchez‑Ares, M., Cameselle‑Teijeiro, J. M., Vázquez‑Estévez, S., Lázaro‑Quintela, M., Vázquez‑Boquete, Á., Afonso‑Afonso, F. J., Casal‑Rubio, J., González‑Piñeiro, A. L., Rico‑Rodríguez, Y., Fírvida‑Pérez, J. L., Ruíz‑Bañobre, J., Couso, E., Santomé, L., Pérez‑Becerra, R., García‑Campelo, R., Amenedo, M., Azpitarte‑Raposeiras, C., Antúnez, J., Abdulkader, I."Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia)". Oncology Letters 12, no. 2 (2016): 1403-1407. https://doi.org/10.3892/ol.2016.4788
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team