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Article

Association between CXCR2 and IL-22BP expression indicate a poor outcome for gastric adenocarcinoma progression

  • Authors:
    • Shi Bin Yang
    • Fanghai Han
    • Jian Hai Wu
    • Zhi Zhao
    • Wenhua Zhan
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China
  • Pages: 1477-1484
    |
    Published online on: June 28, 2016
       https://doi.org/10.3892/ol.2016.4790
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Abstract

C-X-C motif chemokine receptor type 2 (CXCR2), a key regulatory protein, has been associated with multiple roles in the progression of numerous tumors, including gastric adenocarcinoma (GA). However, the mechanism of CXCR2 in the development of tumors remains controversial and unclear. In a previous study, the expression of CXCR2 and interleukin‑22 receptor 2 (IL‑22BP) was observed in GA. This promoted the present study, which aimed to explore the association between the two proteins, and to further analyze their roles in GA. CXCR2 and IL‑22BP protein expression was analyzed by immunohistochemistry and reverse transcription‑quantitative polymerase chain reaction assays in gastric cancer (GC) tissue, additionally confirmed via western blotting and immunocytochemical analysis in the MKN-45, BGC-823 and SGC-7901 cell lines. The association between expression levels and clinicopathological characteristics was evaluated by the Mann‑Whitney U and Kruskal-Wallis tests. Using Kaplan‑Meier plots and Cox proportional hazard models, overall survival (OS) was analyzed. Compared with non‑cancerous tissue, CXCR2 and IL‑22BP were over expressed (P<0.001 and P<0.001, respectively), and were observed mainly in the cytoplasm (P=0.022 and P=0.014, respectively) in GA. The associated protein and messenger RNA levels were analyzed, and coexpression was identified. Increased expression and more positive cases of CXCR2 and IL‑22BP were observed with advanced pathological tumor‑node‑metastasis (p‑TNM) stage in GC (P<0.001 and P<0.001, respectively), as well as the presence and absence of lymph node metastasis (LNM) (P=0.003 and P=0.041, respectively) and deep or superficial muscular invasion (P=0.002 and P=0.004, respectively). In addition, an association between IL‑22BP and tumor diameter was indicated (P=0.021). In a Kaplan‑Meier analysis, compared with negative expression, the two proteins identified a group of patients with the shortest OS. Cox proportional hazard models revealed that the two proteins, in addition to p‑TNM stage, LNM and depth of invasion, predicted a short time to OS. The coexpression of CXCR2 and IL‑22BP was demonstrated in GA, which may indicate that CXCR2 is involved in more complex mechanisms and roles, and indicate a poor outcome in GA progression.
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Copy and paste a formatted citation
Spandidos Publications style
Yang SB, Han F, Wu JH, Zhao Z and Zhan W: Association between CXCR2 and IL-22BP expression indicate a poor outcome for gastric adenocarcinoma progression. Oncol Lett 12: 1477-1484, 2016.
APA
Yang, S.B., Han, F., Wu, J.H., Zhao, Z., & Zhan, W. (2016). Association between CXCR2 and IL-22BP expression indicate a poor outcome for gastric adenocarcinoma progression. Oncology Letters, 12, 1477-1484. https://doi.org/10.3892/ol.2016.4790
MLA
Yang, S. B., Han, F., Wu, J. H., Zhao, Z., Zhan, W."Association between CXCR2 and IL-22BP expression indicate a poor outcome for gastric adenocarcinoma progression". Oncology Letters 12.2 (2016): 1477-1484.
Chicago
Yang, S. B., Han, F., Wu, J. H., Zhao, Z., Zhan, W."Association between CXCR2 and IL-22BP expression indicate a poor outcome for gastric adenocarcinoma progression". Oncology Letters 12, no. 2 (2016): 1477-1484. https://doi.org/10.3892/ol.2016.4790
Copy and paste a formatted citation
x
Spandidos Publications style
Yang SB, Han F, Wu JH, Zhao Z and Zhan W: Association between CXCR2 and IL-22BP expression indicate a poor outcome for gastric adenocarcinoma progression. Oncol Lett 12: 1477-1484, 2016.
APA
Yang, S.B., Han, F., Wu, J.H., Zhao, Z., & Zhan, W. (2016). Association between CXCR2 and IL-22BP expression indicate a poor outcome for gastric adenocarcinoma progression. Oncology Letters, 12, 1477-1484. https://doi.org/10.3892/ol.2016.4790
MLA
Yang, S. B., Han, F., Wu, J. H., Zhao, Z., Zhan, W."Association between CXCR2 and IL-22BP expression indicate a poor outcome for gastric adenocarcinoma progression". Oncology Letters 12.2 (2016): 1477-1484.
Chicago
Yang, S. B., Han, F., Wu, J. H., Zhao, Z., Zhan, W."Association between CXCR2 and IL-22BP expression indicate a poor outcome for gastric adenocarcinoma progression". Oncology Letters 12, no. 2 (2016): 1477-1484. https://doi.org/10.3892/ol.2016.4790
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