Effect of IL-6 and IL-8 on the expression of the complement activation inhibitors MAC-inhibitory protein and decay-accelerating factor in ovarian cancer A2780 cells

Kapka‑Skrzypczak,Lucyna; Popek,Sylwia; Sawicki,Krzysztof; Wolińska,Ewa; Czajka,Magdalena; Skrzypczak,Maciej

doi:10.3892/ol.2016.4795

August-2016 Volume 12 Issue 2

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

August-2016 Volume 12 Issue 2

Full Size Image

Article

Effect of IL-6 and IL-8 on the expression of the complement activation inhibitors MAC-inhibitory protein and decay-accelerating factor in ovarian cancer A2780 cells

Authors:
- Lucyna Kapka‑Skrzypczak
- Sylwia Popek
- Krzysztof Sawicki
- Ewa Wolińska
- Magdalena Czajka
- Maciej Skrzypczak
View Affiliations / Copyright

Affiliations: Department of Medical Biology and Translational Research, Faculty of Medicine, University of Information Technology and Management, 35‑225 Rzeszow, P.R. China, Department of Molecular Biology and Translational Research, Institute of Rural Health, 20‑090 Lublin, Poland, Department of Pathology, Medical University of Warsaw, 02‑091 Warsaw, Poland, Second Department of Gynecology, Medical University of Lublin, 20‑954 Lublin, Poland
Pages: 1507-1512
|
Published online on: June 28, 2016

https://doi.org/10.3892/ol.2016.4795
Expand metrics +

Abstract

The aim of the present study was to evaluate the role of interleukin (IL)-6 and IL-8 on the expression of the membrane-bound complement inhibitors membrane attack complex-inhibitory protein (CD59) and decay-accelerating factor (CD55), in the human ovarian carcinoma A2780 cell line, which is a non‑producing IL‑6 cell line that does exhibit IL‑6 responsiveness, due to the presence of IL‑6 receptors. Extracellular levels of complement system inhibitors were evaluated by western blotting and reverse transcription‑quantitative polymerase chain reaction. Cellular localization of CD55 and CD59 in the ovarian cancer cells was assessed by immunofluorescence. The detection of a soluble form of CD55 and CD59 released by the A2780 cells following stimulation with IL‑6 and IL‑8 was detected by enzyme‑linked immunosorbent assay. The present data revealed that A2780 cells express CD55 and CD59 at the mRNA and protein level, but do not secrete these proteins to the culture medium. Results of western blotting demonstrated that the protein level of CD59 was regulated by IL‑6 and IL‑8 in a dose‑dependent manner. Immunofluorescence analysis revealed that the ovarian cancer A2780 cell line expresses the membrane bound form of CD55 protein. The present results indicate that CD55 and CD59 may affect the efficiency of complement-mediated immunotherapies.

View Figures

View References

1	Fishelson Z, Donin N, Zell S, Schultz S and Kirschfink M: Obstacles to cancer immunotherapy: Expression of membrane complement regulatory proteins (mCRPs) in tumors. Mol Immunol. 40:109–123. 2003. View Article : Google Scholar : PubMed/NCBI
2	Alegretti AP, Mucenic T, Tavares Brenol JC and Xavier RM: The role of CD55/CD59 complement regulatory proteins on peripheral blood cells of systemic lupus erythematosus patients. Rev Bras Rheumatol. 49:276–287. 2009.
3	Li J, Gao MH and Zhang B: Inhibition of mutant CD59 protein on proliferation of ovarian cancer A2780 cells. Ai Zheng. 28:379–383. 2009.(In Chinese). PubMed/NCBI
4	Bjørge L, Hakulinen J, Vintermyr OK, Jarva H, Jensen TS, Iversen OE and Meri S: Ascitic complement system in ovarian cancer. Br J Cancer. 92:895–905. 2005. View Article : Google Scholar : PubMed/NCBI
5	Dzietczenia J, Wróbel T, Mazur G, Poręba R, Jaźwiec B and Kuliczkowski K: Expression of complement regulatory proteins: CD46, CD55, and CD59 and response to rituximab in patients with CD20 (+)non-Hodgkin's lymphoma. Med Oncol. 27:743–746. 2010. View Article : Google Scholar : PubMed/NCBI
6	Liu M, Yang YJ, Zheng H, Zhong XR, Wang Y, Wang Z, Wang YG and Wang YP: Membrane-bound complement regulatory proteins are prognostic factors of operable breast cancer treated with adjuvant trastuzumab: A retrospective study. Oncol Rep. 32:2619–2627. 2014.PubMed/NCBI
7	Shang Y, Chai N, Gu Y, Ding L, Yang Y, Zhou J, Ren G, Hao X, Fan D, Wu K and Nie Y: Systematic immunohistochemical analysis of the expression of CD46, CD55 and CD59 in colon cancer. Arch Pathol Lab Med. 138:910–919. 2014. View Article : Google Scholar : PubMed/NCBI
8	Davidson B and Tropé CG: Ovarian cancer: Diagnostic, biological and prognostic aspects. Womens Health. 10:519–533. 2014.
9	Wcisło G and Szczylik C: Preface. Ovarian Cancer: Pathobiology, Diagnosis and an Overview of Contemporary Methods of Treatment. Wcisło G and Szczylik C: (1st). Termedia. (Poznań). 92011.
10	Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T Robinson, et al: A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment. Cancer Res. 72:66–75. 2012. View Article : Google Scholar : PubMed/NCBI
11	Thibault B, Castells M, Delord JP and Couderc B: Ovarian cancer microenvironment: Implications for cancer dissemination and chemoresistance acquisition. Cancer Metastasis Rev. 33:17–39. 2014. View Article : Google Scholar : PubMed/NCBI
12	Asschert JG, Vellenga E, Ruiters MH and de Vries EG: Regulation of spontaneous and TNF/INF-induced IL-6 expression in two human ovarian-carcinoma cell lines. Int J Cancer. 82:244–249. 1999. View Article : Google Scholar : PubMed/NCBI
13	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
14	Wang Y, Yang J, Gao Y, Du Y, Bao L, Niu W and Yao Z: Regulatory effect of E2, IL-6 and IL-8 on the growth of epithelial ovarian cancer cells. Cell Mol Immunol. 2:365–372. 2005.PubMed/NCBI
15	Beaufort CM, Helmijr JC, Piskorz AM, et al: Ovarian cancer cell line panel (OCCP): Clinical importance of in vitro morphological subset. PLoS One. 9:e1039882014. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of IL-6 and IL-8 on the expression of the complement activation inhibitors MAC-inhibitory protein and decay-accelerating factor in ovarian cancer A2780 cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles