Upregulation of SOX4 antagonizes cellular senescence in esophageal squamous cell carcinoma

Han,Rongfei; Huang,Shiying; Bao,Yonghua; Liu,Xin; Peng,Xiaoyu; Chen,Zhiguo; Wang,Qian; Wang,Jiaqi; Zhang,Qiuping; Wang,Tianfu; Zheng,Duo; Yang,Wancai

doi:10.3892/ol.2016.4799

Upregulation of SOX4 antagonizes cellular senescence in esophageal squamous cell carcinoma

Authors:
- Rongfei Han
- Shiying Huang
- Yonghua Bao
- Xin Liu
- Xiaoyu Peng
- Zhiguo Chen
- Qian Wang
- Jiaqi Wang
- Qiuping Zhang
- Tianfu Wang
- Duo Zheng
- Wancai Yang
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Affiliations: Department of Basic Medicine, School of Medicine, Shenzhen University, Shenzhen, Guangdong 518060, P.R. China, Department of Immunology, School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Surgery, Guangde People's Hospital, Xuancheng, Anhui 242200, P.R. China, Department of Cardiology, The First Affiliated Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China, Department of Pathology, School of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Immunology, School of Basic Medicine, Wuhan University, Wuhan, Hubei 430071, P.R. China
Published online on: June 29, 2016 https://doi.org/10.3892/ol.2016.4799
Pages: 1367-1372
Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Senescence, a terminal cell proliferation arrest that is caused by a variety of cellular stresses such as telomere erosion, DNA damage and oncogenic signaling, is classically considered a tumor defense barrier. However, the mechanism by which cancer cells overcome senescence is undetermined. In this study, the gene expression array data of esophageal squamous cell carcinoma (ESCC) was compared with paired normal tissues and showed that a cohort of genes, including proteinases, chemokines and inflammation factors, are upregulated in ESCC, which exhibits the senescence‑associated secretory phenotype. In addition, reverse transcription‑quantitative polymerase chain reaction was used to demonstrate that gender determining region Y‑box 4 (SOX4) is upregulated in ESCC, and that its expression is inversely correlated with senescence markers. In addition, the knockdown of SOX4 expression by short hairpin RNA decreases ESCC cell proliferation and enhances doxorubicin‑induced cell senescence. These results reveal the presence of a senescent microenvironment in ESCC, and suggest an important antisenescence role of SOX4 in ESCC progression.

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