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Article

Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes

  • Authors:
    • Min‑Dong Du
    • Kai‑Yi He
    • Gang Qin
    • Jin Chen
    • Jin‑Yi Li
  • View Affiliations / Copyright

    Affiliations: Department of Osteoarthrosis, The First Affiliated Hospital of Guangxi Traditional Chinese Medical University, Nanning, Guangxi 530023, P.R. China
  • Pages: 1994-2000
    |
    Published online on: July 14, 2016
       https://doi.org/10.3892/ol.2016.4862
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Abstract

The resistance of cancer cells to chemotherapeutic agents is a major obstacle for successful chemotherapy, and the mechanism of chemoresistance remains unclear. The present study developed an adriamycin-resistant human osteosarcoma MG-63 sub-line (MG-63/ADR), and identified differentially expressed proteins that may be associated with adriamycin resistance. Two dimensional gel electrophoresis, matrix‑assisted laser desorption ionization time‑of‑flight mass spectrometry analysis and a protein identification assay were performed. Western blot analysis was used to examine the prohibitin (PHB) levels in the MG‑63/ADR cells. Quantitative polymerase chain reaction was utilized to detect adriamycin resistant-associated genes. Laser-scanning confocal microscope was employed to examine the colocalization of PHB with v‑myc avian myelocytomatosis viral oncogene homolog (c‑myc), FBJ murine osteosarcoma viral oncogene homolog (c‑fos), tumor protein p53 and retinoblastoma 1 (Rb). In addition, the full length of the open reading frame of human PHB was subcloned into a lentiviral vector pLVX-puro. The proliferative rate of MG‑63 cells was also investigated. The overall protein expression in MG‑63/ADR cells was clearly suppressed. Three notable protein regions, representing high mobility group box 1, Ras homolog gene family, member A, and PHB, were identified to be significantly altered in MG‑63/ADR cells when compared with its parental cells. Therefore, PHB modulated the chemoresistance of MG‑63/ADR cells by interacting with multiple oncogenes or tumor suppressor genes (c‑myc, c‑fos, p53 and Rb). In addition, overexpression of PHB decreases the proliferative rate of MG‑63 cells. In conclusion, PHB is an adriamycin resistance‑associated gene, which may inhibit the proliferation of human osteosarcoma MG-63 cells by interacting with the oncogenes or tumor suppressor genes, c‑myc, c-fos, p53 and Rb.
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Copy and paste a formatted citation
Spandidos Publications style
Du MD, He KY, Qin G, Chen J and Li JY: Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes. Oncol Lett 12: 1994-2000, 2016.
APA
Du, M., He, K., Qin, G., Chen, J., & Li, J. (2016). Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes. Oncology Letters, 12, 1994-2000. https://doi.org/10.3892/ol.2016.4862
MLA
Du, M., He, K., Qin, G., Chen, J., Li, J."Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes". Oncology Letters 12.3 (2016): 1994-2000.
Chicago
Du, M., He, K., Qin, G., Chen, J., Li, J."Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes". Oncology Letters 12, no. 3 (2016): 1994-2000. https://doi.org/10.3892/ol.2016.4862
Copy and paste a formatted citation
x
Spandidos Publications style
Du MD, He KY, Qin G, Chen J and Li JY: Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes. Oncol Lett 12: 1994-2000, 2016.
APA
Du, M., He, K., Qin, G., Chen, J., & Li, J. (2016). Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes. Oncology Letters, 12, 1994-2000. https://doi.org/10.3892/ol.2016.4862
MLA
Du, M., He, K., Qin, G., Chen, J., Li, J."Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes". Oncology Letters 12.3 (2016): 1994-2000.
Chicago
Du, M., He, K., Qin, G., Chen, J., Li, J."Adriamycin resistance-associated prohibitin gene inhibits proliferation of human osteosarcoma MG63 cells by interacting with oncogenes and tumor suppressor genes". Oncology Letters 12, no. 3 (2016): 1994-2000. https://doi.org/10.3892/ol.2016.4862
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