Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
September-2016 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2016 Volume 12 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Autocrine MCP-1/CCR2 signaling stimulates proliferation and migration of renal carcinoma cells

  • Authors:
    • Christoph Küper
    • Franz‑Xaver Beck
    • Wolfgang Neuhofer
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, University of Munich, D‑80336 Munich, Germany, Division of Nephrology and Rheumatology, Clinical Center Traunstein, D-83278 Traunstein, Germany
  • Pages: 2201-2209
    |
    Published online on: July 18, 2016
       https://doi.org/10.3892/ol.2016.4875
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The chemokine monocyte chemoattractant protein-1 [MCP-1; also known as chemokine (C-C motif) ligand 2] is an important mediator of monocyte recruitment during inflammatory processes. Pathologically high expression levels of MCP‑1 by tumor cells have been observed in a variety of cancer types. In the majority of cases, high MCP‑1 expression is associated with a poor prognosis, as infiltration of the tumor with inflammatory monocytes promotes tumor progression and metastasis. MCP‑1 is also expressed in renal cell carcinoma (RCC). In the present study, the function and the regulation of MCP‑1 was investigated in two RCC cell lines, CaKi‑1 and 786‑O. In both cell lines, expression of MCP‑1 was significantly enhanced compared with non‑cancerous control cells. As expected, secretion of MCP‑1 into the medium facilitated the recruitment of peripheral blood monocytes via the chemokine (C‑C motif) receptor type 2 (CCR2). As expression of CCR2 was also detected in 786‑O and CaKi‑1 cells, the effect of autocrine MCP‑1/CCR2 signaling was evaluated in these cells. In proliferation assays, administration of an MCP‑1 neutralizing antibody or of a CCR2 antagonist to CaKi‑1 and 786‑O cells significantly decreased cell growth; supplementation of the growth medium with recombinant human MCP‑1 had no additional effect on proliferation. The migration ability of RCC cells was impaired by MCP-1 neutralization or pharmacological CCR2 inhibition, while it was stimulated by the addition of recombinant human MCP-1, compared with untreated control cells. Finally, substantial differences in the regulation of MCP‑1 expression were observed between RCC cell lines. In CaKi‑1 cells, expression of MCP‑1 appears to be largely mediated by the transcription factor nuclear factor of activated T cells 5, while in 786‑O cells, deletion of the tumor suppressor gene Von-Hippel-Lindau appeared to be responsible for MCP‑1 upregulation, as suggested by previous studies. Taken together, the results of the current study indicate that expression of MCP‑1 in RCC cells promotes tumor progression and metastasis not only by paracrine, but also by autocrine, MCP‑1/CCR2 signaling events, enhancing cell proliferation and migration ability. Therefore, the present findings suggest the MCP-1/CCR2 axis is a potential target for future therapeutic strategies in the treatment of metastatic RCC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Chow WH, Dong LM and Devesa SS: Epidemiology and risk factors for kidney cancer. Nat Rev Urol. 7:245–257. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Linehan WM, Walther MM and Zbar B: The genetic basis of cancer of the kidney. J Urol. 170:2163–2172. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Patel PH, Chadalavada RS, Chaganti RS and Motzer RJ: Targeting von Hippel-Lindau pathway in renal cell carcinoma. Clin Cancer Res. 12:7215–7220. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Motzer RJ: New perspectives on the treatment of metastatic renal cell carcinoma: An introduction and historical overview. Oncologist. 16(Suppl 2): S1–S3. 2011. View Article : Google Scholar

5 

Balkwill F: Cancer and the chemokine network. Nat Rev Cancer. 4:540–550. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Solinas G, Germano G, Mantovani A and Allavena P: Tumor-associated macrophages (TAM) as major players of the cancer-related inflammation. J Leukoc Biol. 86:1065–1073. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Deshmane SL, Kremlev S, Amini S and Sawaya BE: Monocyte chemoattractant protein-1 (MCP-1): An overview. J Interferon Cytokine Res. 29:313–326. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Salcedo R, Ponce ML, Young HA, Wasserman K, Ward JM, Kleinman HK, Oppenheim JJ and Murphy WJ: Human endothelial cells express CCR2 and respond to MCP-1: Direct role of MCP-1 in angiogenesis and tumor progression. Blood. 96:34–40. 2000.PubMed/NCBI

9 

Weber KS, Nelson PJ, Gröne HJ and Weber C: Expression of CCR2 by endothelial cells: Implications for MCP-1 mediated wound injury repair and in vivo inflammatory activation of endothelium. Arterioscler Thromb Vasc Biol. 19:2085–2093. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Ueno T, Toi M, Saji H, Muta M, Bando H, Kuroi K, Koike M, Inadera H and Matsushima K: Significance of macrophage chemoattractant protein-1 in macrophage recruitment, angiogenesis, and survival in human breast cancer. Clin Cancer Res. 6:3282–3289. 2000.PubMed/NCBI

11 

Negus RP, Stamp GW, Hadley J and Balkwill FR: Quantitative assessment of the leukocyte infiltrate in ovarian cancer and its relationship to the expression of C-C chemokines. Am J Pathol. 150:1723–1734. 1997.PubMed/NCBI

12 

Sanford DE, Belt BA, Panni RZ, Mayer A, Deshpande AD, Carpenter D, Mitchem JB, Plambeck-Suess SM, Worley LA and Goetz BD: Inflammatory monocyte mobilization decreases patient survival in pancreatic cancer: A role for targeting the CCL2/CCR2 axis. Clin Cancer Res. 19:3404–3415. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Loberg RD, Ying C, Craig M, Yan L, Snyder LA and Pienta KJ: CCL2 as an important mediator of prostate cancer growth in vivo through the regulation of macrophage infiltration. Neoplasia. 9:556–562. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Daurkin I, Eruslanov E, Stoffs T, Perrin GQ, Algood C, Gilbert SM, Rosser CJ, Su LM, Vieweg J and Kusmartsev S: Tumor-associated macrophages mediate immunosuppression in the renal cancer microenvironment by activating the 15-lipoxygenase-2 pathway. Cancer Res. 71:6400–6409. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Ferrero E, Fabbri M, Poggi A, Galati G, Bernasconi S and Zocchi MR: Tumor-driven matrix invasion by infiltrating lymphocytes: Involvement of the alpha1 integrin I-domain. Eur J Immunol. 28:2530–2536. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Kanno T, Kamba T, Yamasaki T, Shibasaki N, Saito R, Terada N, Toda Y, Mikami Y, Inoue T, Kanematsu A, et al: JunB promotes cell invasion and angiogenesis in VHL-defective renal cell carcinoma. Oncogene. 31:3098–3110. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Yamasaki T, Kamba T, Kanno T, Inoue T, Shibasaki N, Arakaki R, Yamada T, Kondo K, Kamoto T, Nishiyama H, et al: Tumor microvasculature with endothelial fenestrations in VHL null clear cell renal cell carcinomas as a potent target of anti-angiogenic therapy. Cancer Sci. 103:2027–2037. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Lu Y, Cai Z, Galson DL, Xiao G, Liu Y, George DE, Melhem MF, Yao Z and Zhang J: Monocyte chemotactic protein-1 (MCP-1) acts as a paracrine and autocrine factor for prostate cancer growth and invasion. Prostate. 66:1311–1318. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Li C, Liu B, Dai Z and Tao Y: Knockdown of VEGF receptor-1 (VEGFR-1) impairs macrophage infiltration, angiogenesis and growth of clear cell renal cell carcinoma (CRCC). Cancer Biol Ther. 12:872–880. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Kojima R, Taniguchi H, Tsuzuki A, Nakamura K, Sakakura Y and Ito M: Hypertonicity-induced expression of monocyte chemoattractant protein-1 through a novel cis-acting element and MAPK signaling pathways. J Immunol. 184:5253–5262. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Roth I, Leroy V, Kwon HM, Martin PY, Féraille E and Hasler U: Osmoprotective transcription factor NFAT5/TonEBP modulates nuclear factor-kappaB activity. Mol Biol Cell. 21:3459–3474. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Küper C, Beck FX and Neuhofer W: NFAT5-mediated expression of S100A4 contributes to proliferation and migration of renal carcinoma cells. Front Physiol. 5:2932014. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Mosmann T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 65:55–63. 1983. View Article : Google Scholar : PubMed/NCBI

25 

Falk W, Goodwin RH Jr and Leonard EJ: A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration. J Immunol Methods. 33:239–247. 1980. View Article : Google Scholar : PubMed/NCBI

26 

Liang CC, Park AY and Guan JL: In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc. 2:329–333. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:248–254. 1976. View Article : Google Scholar : PubMed/NCBI

28 

Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

29 

Conti I and Rollins BJ: CCL2 (monocyte chemoattractant protein-1) and cancer. Semin Cancer Biol. 14:149–154. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Loberg RD, Day LL, Harwood J, Ying C, St John LN, Giles R, Neeley CK and Pienta KJ: CCL2 is a potent regulator of prostate cancer cell migration and proliferation. Neoplasia. 8:578–586. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Schuler Y, Lee-Thedieck C, Geiger K, Kaiser T, Ino Y, Aicher WK and Klein G: Osteoblast-secreted factors enhance the expression of dysadherin and CCL2-dependent migration of renal carcinoma cells. Int J Cancer. 130:288–299. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Coleman RE: Clinical features of metastatic bone disease and risk of skeletal morbidity. Clin Cancer Res. 12(Suppl): S6243–S6249. 2006. View Article : Google Scholar

33 

Nam JS, Kang MJ, Suchar AM, Shimamura T, Kohn EA, Michalowska AM, Jordan VC, Hirohashi S and Wakefield LM: Chemokine (C-C motif) ligand 2 mediates the prometastatic effect of dysadherin in human breast cancer cells. Cancer Res. 66:7176–7184. 2006. View Article : Google Scholar : PubMed/NCBI

34 

Wyler L, Napoli CU, Ingold B, Sulser T, Heikenwälder M, Schraml P and Moch H: Brain metastasis in renal cancer patients: Metastatic pattern, tumour-associated macrophages and chemokine/chemoreceptor expression. Br J Cancer. 110:686–694. 2014. View Article : Google Scholar : PubMed/NCBI

35 

Wang-Gillam A, Nywening TM, Sanford DE, Lockhart AC, Suresh R, Tan BR, Lim KH, Sorscher S, Fowler K, Amin MA, et al: Phase IB study of FOLFIRINOX plus PF-04136309 in patients with borderline resectable and locally advanced pancreatic adenocarcinoma (PC). J Clin Oncol. 33(Suppl; Abstract 388)2015.PubMed/NCBI

36 

Vivanco I and Sawyers CL: The phosphatidylinositol 3-Kinase AKT pathway in human cancer. Nat Rev Cancer. 2:489–501. 2002. View Article : Google Scholar : PubMed/NCBI

37 

Qian Y, Corum L, Meng Q, Blenis J, Zheng JZ, Shi X, Flynn DC and Jiang BH: PI3K induced actin filament remodeling through Akt and p70S6K1: Implication of essential role in cell migration. Am J Physiol Cell Physiol. 286:C153–C163. 2004. View Article : Google Scholar : PubMed/NCBI

38 

Miyakawa H, Woo SK, Chen CP, Dahl SC, Handler JS and Kwon HM: Cis- and trans-acting factors regulating transcription of the BGT1 gene in response to hypertonicity. Am J Physiol. 274:F753–F761. 1998.PubMed/NCBI

39 

Miyakawa H, Woo SK, Dahl SC, Handler JS and Kwon HM: Tonicity-responsive enhancer binding protein, a rel-like protein that stimulates transcription in response to hypertonicity. Proc Natl Acad Sci USA. 96:2538–2542. 1999. View Article : Google Scholar : PubMed/NCBI

40 

Zhang Z, Ferraris JD, Brooks HL, Brisc I and Burg MB: Expression of osmotic stress-related genes in tissues of normal and hyposmotic rats. Am J Physiol Renal Physiol. 285:F688–F693. 2003. View Article : Google Scholar : PubMed/NCBI

41 

Woo SK, Lee SD, Na KY, Park WK and Kwon HM: TonEBP/NFAT5 stimulates transcription of HSP70 in response to hypertonicity. Mol Cell Biol. 22:5753–5760. 2002. View Article : Google Scholar : PubMed/NCBI

42 

Han KH, Woo SK, Kim WY, Park SH, Cha JH, Kim J and Kwon HM: Maturation of TonEBP expression in developing rat kidney. Am J Physiol Renal Physiol. 287:F878–F885. 2004. View Article : Google Scholar : PubMed/NCBI

43 

López-Rodriguez C, Aramburu J, Jin L, Rakeman AS, Michino M and Rao A: Bridging the NFAT and NF-kappaB families: NFAT5 dimerization regulates cytokine gene transcription in response to osmotic stress. Immunity. 15:47–58. 2001.PubMed/NCBI

44 

Esensten JH, Tsytsykova AV, Lopez-Rodriguez C, Ligeiro FA, Rao A and Goldfeld AE: NFAT5 binds to the TNF promoter distinctly from NFATp, c, 3 and 4, and activates TNF transcription during hypertonic stress alone. Nucleic Acids Res. 33:3845–3854. 2005. View Article : Google Scholar : PubMed/NCBI

45 

Zhong L, Peng X, Hidalgo GE, Doherty DE, Stromberg AJ and Hirschowitz EA: Identification of circulating antibodies to tumor-associated proteins for combined use as markers of non-small cell lung cancer. Proteomics. 4:1216–1225. 2004. View Article : Google Scholar : PubMed/NCBI

46 

Mijatovic T, Mathieu V, Gaussin JF, De Nève N, Ribaucour F, Van Quaquebeke E, Dumont P, Darro F and Kiss R: Cardenolide-induced lysosomal membrane permeabilization demonstrates therapeutic benefits in experimental human non-small cell lung cancers. Neoplasia. 8:402–412. 2006. View Article : Google Scholar : PubMed/NCBI

47 

Levy C, Khaled M, Iliopoulos D, Janas MM, Schubert S, Pinner S, Chen PH, Li S, Fletcher AL, Yokoyama S, et al: Intronic miR-211 assumes the tumor suppressive function of its host gene in melanoma. Mol Cell. 40:841–849. 2010. View Article : Google Scholar : PubMed/NCBI

48 

McCarthy-Keith DM, Malik M, Britten J, Segars J and Catherino WH: Gonadotropin-releasing hormone agonist increases expression of osmotic response genes in leiomyoma cells. Fertil Steril. 95:2383–2387. 2011. View Article : Google Scholar : PubMed/NCBI

49 

Germann S, Gratadou L, Zonta E, Dardenne E, Gaudineau B, Fougère M, Samaan S, Dutertre M, Jauliac S and Auboeuf D: Dual role of the ddx5/ddx17 RNA helicases in the control of the pro-migratory NFAT5 transcription factor. Oncogene. 31:4536–4549. 2012. View Article : Google Scholar : PubMed/NCBI

50 

Jauliac S, López-Rodriguez C, Shaw LM, Brown LF, Rao A and Toker A: The role of NFAT transcription factors in integrin-mediated carcinoma invasion. Nat Cell Biol. 4:540–544. 2002. View Article : Google Scholar : PubMed/NCBI

51 

Chen M, Sinha M, Luxon BA, Bresnick AR and O'Connor KL: Integrin alpha6beta4 controls the expression of genes associated with cell motility, invasion, and metastasis, including S100A4/metastasin. J Biol Chem. 284:1484–1494. 2009. View Article : Google Scholar : PubMed/NCBI

52 

Chen M, Sastry SK and O'Connor KL: Src kinase pathway is involved in NFAT5-mediated S100A4 induction by hyperosmotic stress in colon cancer cells. Am J Physiol Cell Physiol. 300:C1155–C1163. 2011. View Article : Google Scholar : PubMed/NCBI

53 

Alvarez-Diaz S, Valle N, Ferrer-Mayorga G, Lombardía L, Herrera M, Domínguez O, Segura MF, Bonilla F, Hernando E and Muñoz A: MicroRNA-22 is induced by vitamin D and contributes to its antiproliferative, antimigratory and gene regulatory effects in colon cancer cells. Hum Mol Genet. 21:2157–2165. 2012. View Article : Google Scholar : PubMed/NCBI

54 

Slattery ML, Lundgreen A, Bondurant KL and Wolff RK: Tumor necrosis factor-related genes and colon and rectal cancer. Int J Mol Epidemiol Genet. 2:328–338. 2011.PubMed/NCBI

55 

Remo A, Simeone I, Pancione M, Parcesepe P, Finetti P, Cerulo L, Bensmail H, Birnbaum D, Van Laere SJ, Colantuoni V, et al: Systems biology analysis reveals NFAT5 as a novel biomarker and master regulator of inflammatory breast cancer. J Transl Med. 13:1382015. View Article : Google Scholar : PubMed/NCBI

56 

Li JT, Wang LF, Zhao YL, Yang T, Li W, Zhao J, Yu F, Wang L, Meng YL, Liu NN, et al: Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis. Breast Cancer Res. 16:4542014. View Article : Google Scholar : PubMed/NCBI

57 

Lubarski I, Asher C and Garty H: FXYD5 (dysadherin) regulates the paracellular permeability in cultured kidney collecting duct cells. Am J Physiol Renal Physiol. 301:F1270–F1280. 2011. View Article : Google Scholar : PubMed/NCBI

58 

Lubarski I, Pihakaski-Maunsbach K, Karlish SJ, Maunsbach AB and Garty H: Interaction with the Na,K-ATPase and tissue distribution of FXYD5 (related to ion channel). J Biol Chem. 280:37717–37724. 2005. View Article : Google Scholar : PubMed/NCBI

59 

Lim SW, Ahn KO, Sheen MR, Jeon US, Kim J, Yang CW and Kwon HM: Downregulation of renal sodium transporters and tonicity-responsive enhancer binding protein by long-term treatment with cyclosporin A. J Am Soc Nephrol. 18:421–429. 2007. View Article : Google Scholar : PubMed/NCBI

60 

Jeon US, Han KH, Park SH, Lee SD, Sheen MR, Jung JY, Kim WY, Sands JM, Kim J and Kwon HM: Downregulation of renal TonEBP in hypokalemic rats. Am J Physiol Renal Physiol. 293:F408–F415. 2007. View Article : Google Scholar : PubMed/NCBI

61 

Neuhofer W, Woo SK, Na KY, Grunbein R, Park WK, Nahm O, Beck FX and Kwon HM: Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity. Am J Physiol Cell Physiol. 283:C1604–C1611. 2002. View Article : Google Scholar : PubMed/NCBI

62 

Küper C, Beck FX and Neuhofer W: NFAT5 contributes to osmolality-induced MCP-1 expression in mesothelial cells. Mediators Inflamm. 2012:5130152012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Küper C, Beck FX and Neuhofer W: Autocrine MCP-1/CCR2 signaling stimulates proliferation and migration of renal carcinoma cells. Oncol Lett 12: 2201-2209, 2016.
APA
Küper, C., Beck, F., & Neuhofer, W. (2016). Autocrine MCP-1/CCR2 signaling stimulates proliferation and migration of renal carcinoma cells. Oncology Letters, 12, 2201-2209. https://doi.org/10.3892/ol.2016.4875
MLA
Küper, C., Beck, F., Neuhofer, W."Autocrine MCP-1/CCR2 signaling stimulates proliferation and migration of renal carcinoma cells". Oncology Letters 12.3 (2016): 2201-2209.
Chicago
Küper, C., Beck, F., Neuhofer, W."Autocrine MCP-1/CCR2 signaling stimulates proliferation and migration of renal carcinoma cells". Oncology Letters 12, no. 3 (2016): 2201-2209. https://doi.org/10.3892/ol.2016.4875
Copy and paste a formatted citation
x
Spandidos Publications style
Küper C, Beck FX and Neuhofer W: Autocrine MCP-1/CCR2 signaling stimulates proliferation and migration of renal carcinoma cells. Oncol Lett 12: 2201-2209, 2016.
APA
Küper, C., Beck, F., & Neuhofer, W. (2016). Autocrine MCP-1/CCR2 signaling stimulates proliferation and migration of renal carcinoma cells. Oncology Letters, 12, 2201-2209. https://doi.org/10.3892/ol.2016.4875
MLA
Küper, C., Beck, F., Neuhofer, W."Autocrine MCP-1/CCR2 signaling stimulates proliferation and migration of renal carcinoma cells". Oncology Letters 12.3 (2016): 2201-2209.
Chicago
Küper, C., Beck, F., Neuhofer, W."Autocrine MCP-1/CCR2 signaling stimulates proliferation and migration of renal carcinoma cells". Oncology Letters 12, no. 3 (2016): 2201-2209. https://doi.org/10.3892/ol.2016.4875
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team