Expression of early growth response gene‑1 in precancerous lesions of gastric cancer

Park,Seon‑Young; Kim,Ji‑Young; Lee,Su‑Mi; Chung,Jin Ook; Lee,Kyung‑Hwa; Jun,Chung‑Hwan; Park,Chang‑Hwan; Kim,Hyun‑Soo; Choi,Sung‑Kyu; Rew,Jong‑Sun; Jung,Young‑Do; Lee,Yong Han

doi:10.3892/ol.2016.4962

October-2016 Volume 12 Issue 4

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October-2016 Volume 12 Issue 4

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Article

Expression of early growth response gene‑1 in precancerous lesions of gastric cancer

Authors:
- Seon‑Young Park
- Ji‑Young Kim
- Su‑Mi Lee
- Jin Ook Chung
- Kyung‑Hwa Lee
- Chung‑Hwan Jun
- Chang‑Hwan Park
- Hyun‑Soo Kim
- Sung‑Kyu Choi
- Jong‑Sun Rew
- Young‑Do Jung
- Yong Han Lee
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Affiliations: Department of Gastroenterology and Hepatology, Chonnam National University Medical School, Gwangju 501‑757, Republic of Korea, Department of Endocrinology, Chonnam National University Medical School, Gwangju 501‑757, Republic of Korea, Department of Pathology, Chonnam National University Research Institute of Medical Sciences, Chonnam National University Medical School, Gwangju 501‑757, Republic of Korea, Department of Biomedical Science and Technology, Chonnam National University Medical School, Gwangju 501‑757, Republic of Korea, Department of Biological Sciences, College of Biological Science and Biotechnology, Konkuk University, Seoul 143‑701, Republic of Korea
Pages: 2710-2715
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Published online on: August 5, 2016

https://doi.org/10.3892/ol.2016.4962
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Abstract

Several studies have demonstrated a correlation between the expression of early growth response gene‑1 (EGR‑1) and the progression of gastric cancers at advanced stages. However, the effects of EGR‑1 expression on human gastric cancer progression, particularly on precancerous lesions, have not been investigated. In this study, we evaluate EGR‑1 expression levels in target mucosa from patients with early gastric cancer and precancerous lesions, and assess whether EGR‑1 expression affects the oncogenic phenotypes of human gastric cancer cells. EGR‑1 protein levels were measured in tissues from subjects with normal mucosa (n=6), low‑grade dysplasia (n=6), high‑grade dysplasia (n=4) and adenocarcinoma (n=3) using enzyme‑linked immunosorbent assay and immunohistochemistry analyses. We also investigated the role of EGR‑1 in tumor cell behavior by transiently expressing a dominant active EGR‑1 variant in cultured cells. A positive correlation was observed between EGR‑1 expression and gastric carcinogenesis (P=0.016). Furthermore, there was an increase in nuclear and cytoplasmic expression of EGR‑1 in accordance with the histological grade (P for trends=0.003 and 0.003, respectively), and a positive association between the sum of the nuclear and cytoplasmic EGR‑1 expression values and the histological grade (P=0.003). In addition, transient overexpression of EGR‑1 enhanced cell proliferation, stimulated cell migration, and promoted the phosphorylation of p38 MAPK and AKT in gastric cancer cells in vitro. Our findings demonstrate that EGR‑1 may contribute to the early stages of gastric carcinogenesis via the alteration of tumor cell behaviors.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression of early growth response gene‑1 in precancerous lesions of gastric cancer

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