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Article

Discovery of genes from feces correlated with colorectal cancer progression

  • Authors:
    • Chia‑Long Lee
    • Chi‑Jung Huang
    • Shung‑Haur Yang
    • Chun‑Chao Chang
    • Chi‑Cheng Huang
    • Chih‑Cheng Chien
    • Ruey‑Neng Yang
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Taipei Medical University, Taipei 11031, Taiwan, R.O.C., Department of Medical Research, Cathay General Hospital, Taipei 10630, Taiwan, R.O.C., Department of Surgery, Taipei Veterans General Hospital, Taipei 11217, Taiwan, R.O.C., Department of Nursing, Ching Kuo Institute of Management and Health, Keelung 20301, Taiwan, R.O.C.
  • Pages: 3378-3384
    |
    Published online on: August 31, 2016
       https://doi.org/10.3892/ol.2016.5069
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Abstract

Colorectal cancer (CRC) is considered to develop slowly via a progressive accumulation of genetic mutations. Markers of CRC may serve to provide the basis for decision‑making, and may assist in cancer prevention, detection and prognostic prediction. DNA and messenger (m)RNA molecules that are present in human feces faithfully represent CRC manifestations. In the present study, exogenous mouse cells verified the feasibility of total fecal RNA as a marker of CRC. Furthermore, five significant genes encoding solute carrier family 15, member 4 (SLC15A4), cluster of differentiation (CD)44, 3-oxoacid CoA-transferase 1 (OXCT1), placenta‑specific 8 (PLAC8) and growth arrest‑specific 2 (GAS2), which are differentially expressed in the feces of CRC patients, were verified in different CRC cell lines using quantitative polymerase chain reaction. The present study demonstrated that the mRNA level of SLC15A4 was increased in the majority of CRC cell lines evaluated (SW1116, LS123, Caco‑2 and T84). An increased level of CD44 mRNA was only detected in an early‑stage CRC cell line, SW1116, whereas OXCT1 was expressed at higher levels in the metastatic CRC cell line CC‑M3. In addition, two genes, PLAC8 and GAS2, were highly expressed in the recurrent CRC cell line SW620. Genes identified in the feces of CRC patients differed according to their clinical characteristics, and this differential expression was also detected in the corresponding CRC cell lines. In conclusion, feces represent a good marker of CRC and can be interpreted through the appropriate CRC cell lines.
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Lee CL, Huang CJ, Yang SH, Chang CC, Huang CC, Chien CC and Yang RN: Discovery of genes from feces correlated with colorectal cancer progression. Oncol Lett 12: 3378-3384, 2016.
APA
Lee, C., Huang, C., Yang, S., Chang, C., Huang, C., Chien, C., & Yang, R. (2016). Discovery of genes from feces correlated with colorectal cancer progression. Oncology Letters, 12, 3378-3384. https://doi.org/10.3892/ol.2016.5069
MLA
Lee, C., Huang, C., Yang, S., Chang, C., Huang, C., Chien, C., Yang, R."Discovery of genes from feces correlated with colorectal cancer progression". Oncology Letters 12.5 (2016): 3378-3384.
Chicago
Lee, C., Huang, C., Yang, S., Chang, C., Huang, C., Chien, C., Yang, R."Discovery of genes from feces correlated with colorectal cancer progression". Oncology Letters 12, no. 5 (2016): 3378-3384. https://doi.org/10.3892/ol.2016.5069
Copy and paste a formatted citation
x
Spandidos Publications style
Lee CL, Huang CJ, Yang SH, Chang CC, Huang CC, Chien CC and Yang RN: Discovery of genes from feces correlated with colorectal cancer progression. Oncol Lett 12: 3378-3384, 2016.
APA
Lee, C., Huang, C., Yang, S., Chang, C., Huang, C., Chien, C., & Yang, R. (2016). Discovery of genes from feces correlated with colorectal cancer progression. Oncology Letters, 12, 3378-3384. https://doi.org/10.3892/ol.2016.5069
MLA
Lee, C., Huang, C., Yang, S., Chang, C., Huang, C., Chien, C., Yang, R."Discovery of genes from feces correlated with colorectal cancer progression". Oncology Letters 12.5 (2016): 3378-3384.
Chicago
Lee, C., Huang, C., Yang, S., Chang, C., Huang, C., Chien, C., Yang, R."Discovery of genes from feces correlated with colorectal cancer progression". Oncology Letters 12, no. 5 (2016): 3378-3384. https://doi.org/10.3892/ol.2016.5069
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