Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
November-2016 Volume 12 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2016 Volume 12 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients

  • Authors:
    • Katarzyna Jakubowska
    • Anna Pryczynicz
    • Violetta Dymicka‑Piekarska
    • Waldemar Famulski
    • Katarzyna Guzińska‑Ustymowicz
  • View Affiliations / Copyright

    Affiliations: Department of General Pathomorphology, Medical University of Białystok, Białystok 15‑269, Poland, Department of Clinical Laboratory Diagnostics, Medical University of Białystok, Białystok 15‑269, Poland, Department of Medical Pathomorphology, Medical University of Białystok, Białystok 15‑269, Poland
  • Pages: 3591-3597
    |
    Published online on: September 1, 2016
       https://doi.org/10.3892/ol.2016.5075
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Survivin is one of the apoptosis-related inhibitors that is associated with a more aggressive behavior and a poor prognosis in numerous types of malignancies, including colorectal cancer (CRC). The objective of the present study was to perform immunohistochemical tissue analysis of survivin expression and serum analysis of survivin levels in CRC patients. The study group consisted of 55 CRC patients. Survivin expression was assessed by immunohistochemistry in 38 patients using monoclonal antibodies. Color reactions were observed in the nucleus and cytoplasm of the cancer cells. The expression was defined based on the H‑score method. The level of survivin was determined using the enzyme-linked immunosorbent assay method. A positive immunoreaction was observed in the tumor tissues of 84.2% (32/38) of patients with CRC, consisting of nuclear (63.2%; 24/38) and cytoplasmic (81.6%; 31/38) expression. The survivin nuclear expression was associated with tumor mass location and the presence of distant metastases (P=0.048 and P=0.026, respectively). Survivin was detected in the sera of 38.2% (21/55) of CRC patients and in 81.8% (18/22) of healthy individuals. Serum protein levels were found to correlate with hematocrit (P=0.035), hemoglobin (P=0.008) and albumin (P=0.045), but not with any of the investigated clinicopathological parameters. The immunohistochemical positive reaction of survivin in the nuclei of cancer cells may condition their proliferative capacity, which is associated with higher risk of developing metastatic foci. Thus, the present study suggests that the expression of survivin may have diagnostic implications in cancer of the colon and thus requires further research. By contrast, the survivin serum level in CRC patients appears to be diagnostically ineffectual for clinical use.
View Figures

Figure 1

Figure 2

View References

1 

Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Pepe MS, Etzioni R, Feng Z, Potter JD, Thompson ML, Thornquist M, Winget M and Yasui Y: Phases of biomarker development for early detection of cancer. J Natl Cancer Inst. 93:1054–1061. 2001. View Article : Google Scholar : PubMed/NCBI

3 

Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Walczak H and Krammer PH: The CD95 (APO-1/Fas) and the TRAIL (APO-2L) apoptosis systems. Exp Cell Res. 256:58–66. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Thomadaki H and Scorilas A: BCL2 family of apoptosis-related genes: Functions and clinical implications in cancer. Crit Rev Clin Lab Sci. 43:1–67. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Sträter J, Hinz U, Hasel C, Bhanot U, Mechtersheimer G, Lehnert T and Möller P: Impaired CD95 expression predisposes for recurrence in curatively resected colon carcinoma: Clinical evidence for immunoselection and CD95L mediated control of minimal residual disease. Gut. 54:661–665. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Korkolopoulou P, Saetta AA, Levidou G, Gigelou F, Lazaris A, Thymara I, Scliri M, Bousboukea K, Michalopoulos NV, Apostolikas N, et al: c-FLIP expression in colorectal carcinomas: Association with Fas/FasL expression and prognostic implications. Histopathology. 51:150–156. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Strand S, Hofmann WJ, Hug H, Müller M, Otto G, Strand D, Mariani SM, Stremmel W, Krammer PH and Galle PR: Lymphocyte apoptosis induced by CD95 (APO-1/Fas) ligand-expressing tumor cells-a mechanism of immune evasion? Nat Med. 2:1361–1366. 1996. View Article : Google Scholar : PubMed/NCBI

9 

Deveraux QL, Stennicke HR, Salvesen GS and Reed JC: Endogenous inhibitors of caspases. J Clin Immunol. 19:388–398. 1999. View Article : Google Scholar : PubMed/NCBI

10 

Chantalat L, Skoufias DA, Kleman JP, Jung B, Dideberg O and Margolis RL: Crystal structure of human survivin reveals a bow tie-shaped dimer with two unusual alpha-helical extensions. Mol Cell. 6:183–189. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Dohi T, Okada K, Xia F, Wilford CE, Samuel T, Welsh K, Marusawa H, Zou H, Armstrong R, Matsuzawa S, et al: An IAP-IAP complex inhibits apoptosis. J Biol Chem. 279:34087–34090. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Ambrosini G, Adida C and Altieri DC: A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med. 3:917–921. 1997. View Article : Google Scholar : PubMed/NCBI

13 

Mahotka C, Wenzel M, Springer E, Gabbert HE and Gerharz CD: Survivin-deltaEx3 and survivin-2B: Two novel splice variants of the apoptosis inhibitor survivin with different antiapoptotic properties. Cancer Res. 59:6097–6102. 1999.PubMed/NCBI

14 

Hamilton SR and Aaltonen LA: World Health Organization Classification of TumoursPathology and Genetics of Tumours of the Digestive System. IARC Press; Lyon: pp. 1042000

15 

Kalliakmanis JG, Kouvidou Ch, Latoufis C, Kouvatseas G, Anagnostakis D, Papatheodoridis G, Koskinas J and Archimandritis A: Survivin expression in colorectal carcinomas: Correlations with clinicopathological parameters and survival. Dig Dis Sci. 55:2958–2964. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Choi J and Chang H: The expression of MAGE and SSX, and correlation of COX2, VEGF, and survivin in colorectal cancer. Anticancer Res. 32:559–564. 2012.PubMed/NCBI

17 

Hernandez JM, Farma JM, Coppola D, Hakam A, Fulp WJ, Chen DT, Siegel EM, Yeatman TJ and Shibata D: Expression of the antiapoptotic protein survivin in colon cancer. Clin Colorectal Cancer. 10:188–193. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Xi RC, Biao WS and Gang ZZ: Significant elevation of survivin and livin expression in human colorectal cancer: Inverse correlation between expression and overall survival. Onkologie. 34:428–432. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Ponnelle T, Chapusot C, Martin L, Bouvier AM, Plenchette S, Faivre J, Solary E and Piard F: Cellular localisation of survivin: Impact on the prognosis in colorectal cancer. J Cancer Res Clin Oncol. 131:504–510. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Shintani M, Sangawa A, Yamao N and Kamoshida S: Immunohistochemical expression of nuclear and cytoplasmic survivin in gastrointestinal carcinoma. Int J Clin Exp Pathol. 6:2919–2927. 2013.PubMed/NCBI

21 

Qi G, Tuncel H, Aoki E, Tanaka S, Oka S, Kaneko I, Okamoto M, Tatsuka M, Nakai S and Shimamoto F: Intracellular localization of survivin determines biological behavior in colorectal cancer. Oncol Rep. 22:557–562. 2009.PubMed/NCBI

22 

McKenzie JA, Liu T, Goodson AG and Grossman D: Survivin enhances motility of melanoma cells by supporting Akt activation and {alpha}5 integrin upregulation. Cancer Res. 70:7927–7937. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Zhang M, Coen JJ, Suzuki Y, Siedow MR, Niemierko A, Khor LY, Pollack A, Zhang Y, Zietman AL, Shipley WU and Chakravarti A: Survivin is a potential mediator of prostate cancer metastasis. Int J Radiat Oncol Biol Phys. 78:1095–1103. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Chu XY, Chen LB, Wang JH, Su QS, Yang JR, Lin Y, Xue LJ, Liu XB and Mo XB: Overexpression of survivin is correlated with increased invasion and metastasis of colorectal cancer. J Surg Oncol. 105:520–528. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Xiaoyuan C, Longbang C, Jinghua W, Xiaoxiang G, Huaicheng G, Qun Z and Haizhu S: Survivin: A potential prognostic marker and chemoradiotherapeutic target for colorectal cancer. Ir J Med Sci. 179:327–335. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Shen W, Tu JK, Wang XH and Fu ZX: Oncolytic adenovirus mediated Survivin RNA interference and 5-fluorouracil synergistically suppress the lymphatic metastasis of colorectal cancer. Oncol Rep. 24:1285–1290. 2010.PubMed/NCBI

27 

Li JH, He WJ and He YJ: Expression and clinical significance of Survivin and Livin in DukesoB colorectal cancer. Ai Zheng. 26:547–551. 2007.(In Chinese). PubMed/NCBI

28 

Lee YY, Yu CP, Lin CK, Nieh S, Hsu KF, Chiang H and Jin JS: Expression of survivin and cortactin in colorectal adenocarcinoma: Association with clinicopathological parameters. Dis Markers. 26:9–18. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Suzuki A, Hayashida M, Ito T, Kawano H, Nakano T, Miura M, Akahane K and Shiraki K: Survivin initiates cell cycle entry by the competitive interaction with Cdk4/p16(INK4a) and Cdk2/cyclin E complex activation. Oncogene. 19:3225–3234. 2000. View Article : Google Scholar : PubMed/NCBI

30 

Yagihashi A, Asanuma K, Nakamura M, Araya J, Mano Y, Torigoe T, Kobayashi D and Watanabe N: Detection of anti-survivin antibody in gastrointestinal cancer patients. Clin Chem. 47:1729–1731. 2001.PubMed/NCBI

31 

Wang YQ, Zhang HH, Liu CL, Xia Q, Wu H, Yu XH and Kong W: Correlation between auto-antibodies to survivin and MUC1 variable number tandem repeats in colorectal cancer. Asian Pac J Cancer Prev. 13:5557–5562. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Rohayem J, Diestelkoetter P, Weigle B, Oehmichen A, Schmitz M, Mehlhorn J, Conrad K and Rieber EP: Antibody response to the tumor-associated inhibitor of apoptosis protein survivin in cancer patients. Cancer Res. 60:1815–1817. 2000.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jakubowska K, Pryczynicz A, Dymicka‑Piekarska V, Famulski W and Guzińska‑Ustymowicz K: Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients. Oncol Lett 12: 3591-3597, 2016.
APA
Jakubowska, K., Pryczynicz, A., Dymicka‑Piekarska, V., Famulski, W., & Guzińska‑Ustymowicz, K. (2016). Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients. Oncology Letters, 12, 3591-3597. https://doi.org/10.3892/ol.2016.5075
MLA
Jakubowska, K., Pryczynicz, A., Dymicka‑Piekarska, V., Famulski, W., Guzińska‑Ustymowicz, K."Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients". Oncology Letters 12.5 (2016): 3591-3597.
Chicago
Jakubowska, K., Pryczynicz, A., Dymicka‑Piekarska, V., Famulski, W., Guzińska‑Ustymowicz, K."Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients". Oncology Letters 12, no. 5 (2016): 3591-3597. https://doi.org/10.3892/ol.2016.5075
Copy and paste a formatted citation
x
Spandidos Publications style
Jakubowska K, Pryczynicz A, Dymicka‑Piekarska V, Famulski W and Guzińska‑Ustymowicz K: Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients. Oncol Lett 12: 3591-3597, 2016.
APA
Jakubowska, K., Pryczynicz, A., Dymicka‑Piekarska, V., Famulski, W., & Guzińska‑Ustymowicz, K. (2016). Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients. Oncology Letters, 12, 3591-3597. https://doi.org/10.3892/ol.2016.5075
MLA
Jakubowska, K., Pryczynicz, A., Dymicka‑Piekarska, V., Famulski, W., Guzińska‑Ustymowicz, K."Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients". Oncology Letters 12.5 (2016): 3591-3597.
Chicago
Jakubowska, K., Pryczynicz, A., Dymicka‑Piekarska, V., Famulski, W., Guzińska‑Ustymowicz, K."Immunohistochemical expression and serum level of survivin protein in colorectal cancer patients". Oncology Letters 12, no. 5 (2016): 3591-3597. https://doi.org/10.3892/ol.2016.5075
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team