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Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy

  • Authors:
    • Masako Shida
    • Masanori Yasuda
    • Mariko Fujita
    • Masaki Miyazawa
    • Hiroshi Kajiwara
    • Takeshi Hirasawa
    • Masae Ikeda
    • Naruaki Matsui
    • Toshinari Muramatsu
    • Mikio Mikami
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Tokai University School of Medicine, Isehara, Kanagawa 259‑1193, Japan, Department of Pathology, Saitama Medical University International Medical Center, Hidaka, Saitama 350‑1298, Japan, Department of Pathology, Tokai University School of Medicine, Isehara, Kanagawa 259‑1193, Japan, Department of Pathology, Tokai University Oiso Hospital, Oiso, Kanagawa 259‑0198, Japan, Department of Obstetrics and Gynecology, Tokai University Hachioji Hospital, Hachioji, Tokyo 192‑0032, Japan
    Copyright: © Shida et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3215-3223
    |
    Published online on: September 2, 2016
       https://doi.org/10.3892/ol.2016.5082
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Abstract

Thymidine phosphorylase (TP) is structurally similar to platelet-derived endothelial cell growth factor, and it activates 5‑fluorouracil (5‑FU) prodrugs and also promotes angiogenesis. In the present study, the possibility of using TP expression as a biomarker for 5‑FU prodrugs, and the significance of TP as an angiogenic factor, were investigated in patients with gynecological tumors. The subjects enrolled in the study were 188 patients with gynecological tumors who provided informed consent and underwent tumor resection at the Department of Obstetrics and Gynecology of Tokai University Hospital between February 2002 and January 2010. Measurement of the enzymatic activity of TP and dihydropyrimidine dehydrogenase (DPD) was performed by enzyme‑linked immunosorbent assay. In addition, immunohistochemistry (IHC) analysis of microvessels by monochrome imaging, western blotting and reverse transcription-polymerase chain reaction were performed. The mean TP activity and the TP/DPD ratio were increased in squamous cell carcinoma of the cervix (306.9 and 2.2 U/mg protein, respectively) and adenosquamous carcinoma (317.6 and 1.4 U/mg protein, respectively) compared with benign tumors and other malignancies, including endometrial (uterine) carcinoma, ovarian serous adenocarcinoma and ovarian mucinous adenocarcinoma. However, these parameters were also elevated in other histological types of cancer such as clear cell adenocarcinoma of the ovary (115.2 and 2.1 U/mg protein, respectively), in which the microvessel area was the largest of all the histological types analyzed. Since high TP expression and a high TP/DPD ratio were identified in other tumors besides cervical cancer, it is possible that patients for whom 5‑FU prodrugs are indicated could be selected appropriately if their TP activity is determined and their TP expression is analyzed by IHC prior to initiation of the treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Shida M, Yasuda M, Fujita M, Miyazawa M, Kajiwara H, Hirasawa T, Ikeda M, Matsui N, Muramatsu T, Mikami M, Mikami M, et al: Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy. Oncol Lett 12: 3215-3223, 2016.
APA
Shida, M., Yasuda, M., Fujita, M., Miyazawa, M., Kajiwara, H., Hirasawa, T. ... Mikami, M. (2016). Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy. Oncology Letters, 12, 3215-3223. https://doi.org/10.3892/ol.2016.5082
MLA
Shida, M., Yasuda, M., Fujita, M., Miyazawa, M., Kajiwara, H., Hirasawa, T., Ikeda, M., Matsui, N., Muramatsu, T., Mikami, M."Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy". Oncology Letters 12.5 (2016): 3215-3223.
Chicago
Shida, M., Yasuda, M., Fujita, M., Miyazawa, M., Kajiwara, H., Hirasawa, T., Ikeda, M., Matsui, N., Muramatsu, T., Mikami, M."Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy". Oncology Letters 12, no. 5 (2016): 3215-3223. https://doi.org/10.3892/ol.2016.5082
Copy and paste a formatted citation
x
Spandidos Publications style
Shida M, Yasuda M, Fujita M, Miyazawa M, Kajiwara H, Hirasawa T, Ikeda M, Matsui N, Muramatsu T, Mikami M, Mikami M, et al: Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy. Oncol Lett 12: 3215-3223, 2016.
APA
Shida, M., Yasuda, M., Fujita, M., Miyazawa, M., Kajiwara, H., Hirasawa, T. ... Mikami, M. (2016). Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy. Oncology Letters, 12, 3215-3223. https://doi.org/10.3892/ol.2016.5082
MLA
Shida, M., Yasuda, M., Fujita, M., Miyazawa, M., Kajiwara, H., Hirasawa, T., Ikeda, M., Matsui, N., Muramatsu, T., Mikami, M."Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy". Oncology Letters 12.5 (2016): 3215-3223.
Chicago
Shida, M., Yasuda, M., Fujita, M., Miyazawa, M., Kajiwara, H., Hirasawa, T., Ikeda, M., Matsui, N., Muramatsu, T., Mikami, M."Possible role of thymidine phosphorylase in gynecological tumors as an individualized treatment strategy". Oncology Letters 12, no. 5 (2016): 3215-3223. https://doi.org/10.3892/ol.2016.5082
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