Expression and clinicopathological significance of the lncRNA HOXA11-AS in colorectal cancer

Li,Tong; Xu,Chengfei; Cai,Bin; Zhang,Meng; Gao,Feng; Gan,Jialiang

doi:10.3892/ol.2016.5129

Expression and clinicopathological significance of the lncRNA HOXA11-AS in colorectal cancer

Authors:
- Tong Li
- Chengfei Xu
- Bin Cai
- Meng Zhang
- Feng Gao
- Jialiang Gan
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Affiliations: Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
Published online on: September 14, 2016 https://doi.org/10.3892/ol.2016.5129
Pages: 4155-4160

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Abstract

HOXA11 antisense RNA (HOXA11-AS) is a long non‑coding RNA (lncRNA) that is important in determining cancer progression. HOXA11-AS was recently identified as a novel biomarker in lung cancer progression. However, its role in colorectal cancer (CRC) remains poorly understood. The present study aimed to analyze lncRNA HOXA11‑AS expression in CRC and investigate a possible association between HOXA11‑AS and clinicopathological factors. HOXA11‑AS expression was examined by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in 84 CRC tissues and adjacent non‑cancerous tissues, in addition to 3 CRC cell lines and 1 human normal colorectal cell line. The results demonstrated that HOXA11‑AS expression was decreased in the CRC tissues and cell lines compared with that of the controls (P<0.05). Clinicopathological analysis indicated that low HOXA11‑AS expression was significantly correlated with tumor size, advanced tumor‑node‑metastasis stage, lymph node metastasis and carcinoembryonic antigen level of patients with CRC (P<0.05). Furthermore, the areas under the curve (AUC) were 0.613 and 0.628 for HOXA11‑AS, indicating that the lncRNA is able to distinguish CRC tissue from non‑cancerous tissue, and CRC tissue with lymph node metastasis from CRC without lymph node metastasis. Therefore, HOXA11‑AS may function as a potential biomarker and target for novel therapeutic strategies to treat CRC.

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