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Article

Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways

Corrigendum in: /10.3892/ol.2024.14225
  • Authors:
    • Rui Zhang
    • Ming Lu
    • Zhen Zhang
    • Xiliang Tian
    • Shouyu Wang
    • Decheng Lv
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China
  • Pages: 4147-4154
    |
    Published online on: September 14, 2016
       https://doi.org/10.3892/ol.2016.5136
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Abstract

The present study aimed to investigate the reversal effect of resveratrol on the phenomenon of multidrug resistance in U2OS/adriamycin (ADR) cells and to clarify the molecular mechanisms. To examine the cell survival and half‑inhibitory concentration (IC50) of ADR in U2OS and U2OS/ADR cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used. The accumulation of ADR in U2OS and U2OS/ADR cells was investigated by flow cytometry. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were used to detect the expression of multidrug resistance protein 1 (MDR1), P‑glycoprotein (P‑gp), p65 and p38. Compared with U2OS cells, the IC50 value of ADR was significantly increased in U2OS/ADR cells, which exhibited high levels of MDR1/P‑gp. However, resveratrol could drastically reduce the IC50 value of ADR and the expression of MDR1/P‑gp, and increased the accumulation of ADR in U2OS/ADR cells. In addition, the expression levels of p38 (phosphorylated) and p65 (acetylated and total) in U2OS/ADR cells were also significantly suppressed by resveratrol. These results suggested that the nuclear factor (NF)‑κB and p38 mitogen-activated protein kinase (MAPK) signaling pathways are correlated with ADR‑induced drug resistance in U2OS/ADR cells. Furthermore, resveratrol could downregulate the expression of MDR1/P‑gp and reverse the drug resistance phenomenon in U2OS/ADR cells partly at least by suppressing the activation of the NF‑κB and p38 MAPK signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang R, Lu M, Zhang Z, Tian X, Wang S and Lv D: Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways Corrigendum in /10.3892/ol.2024.14225. Oncol Lett 12: 4147-4154, 2016.
APA
Zhang, R., Lu, M., Zhang, Z., Tian, X., Wang, S., & Lv, D. (2016). Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways Corrigendum in /10.3892/ol.2024.14225. Oncology Letters, 12, 4147-4154. https://doi.org/10.3892/ol.2016.5136
MLA
Zhang, R., Lu, M., Zhang, Z., Tian, X., Wang, S., Lv, D."Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways Corrigendum in /10.3892/ol.2024.14225". Oncology Letters 12.5 (2016): 4147-4154.
Chicago
Zhang, R., Lu, M., Zhang, Z., Tian, X., Wang, S., Lv, D."Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways Corrigendum in /10.3892/ol.2024.14225". Oncology Letters 12, no. 5 (2016): 4147-4154. https://doi.org/10.3892/ol.2016.5136
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang R, Lu M, Zhang Z, Tian X, Wang S and Lv D: Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways Corrigendum in /10.3892/ol.2024.14225. Oncol Lett 12: 4147-4154, 2016.
APA
Zhang, R., Lu, M., Zhang, Z., Tian, X., Wang, S., & Lv, D. (2016). Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways Corrigendum in /10.3892/ol.2024.14225. Oncology Letters, 12, 4147-4154. https://doi.org/10.3892/ol.2016.5136
MLA
Zhang, R., Lu, M., Zhang, Z., Tian, X., Wang, S., Lv, D."Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways Corrigendum in /10.3892/ol.2024.14225". Oncology Letters 12.5 (2016): 4147-4154.
Chicago
Zhang, R., Lu, M., Zhang, Z., Tian, X., Wang, S., Lv, D."Resveratrol reverses P-glycoprotein-mediated multidrug resistance of U2OS/ADR cells by suppressing the activation of the NF-κB and p38 MAPK signaling pathways Corrigendum in /10.3892/ol.2024.14225". Oncology Letters 12, no. 5 (2016): 4147-4154. https://doi.org/10.3892/ol.2016.5136
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